A variety of techniques from molecular biology have been used in research laboratories to study the effects of DNA binding small molecules, such as the gel mobility shift assay,~1H NMR, DNA foot-printing assay, and fluorescence-based assays, UV-Vis spectrum and viscometry.

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By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

為研制帶有O型口蹄疫病毒(Foot-and-Mouth Disease Virus欧美视频欧美性爱，F(xiàn)MDV)China99株結構蛋白基因及多個非結構蛋白基因的DNA疫苗，本研究通過定點突變方法和PCR擴增方法人妻专区，獲得包含有FMDV China99株結構蛋白P1无码视频亚洲一区、非結構蛋白2A、3C以及部分2B伊人国产精品、3B編碼基因的片段P12X3C和包含有FMDV China99株結構蛋白P1污污污网站免费、非結構蛋白2A、3C亚洲日韩精品无码专区网站、3D以及部分2B影音先锋AV每日在线、3B編碼基因的片段P12X3C3D，將獲得的基因片段直接/酶切后與同樣處理的真核表達質(zhì)粒連接和孕妇疯狂作爱视频，分別得到重組質(zhì)粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D国产经是品久久久久久久久免费、pTARGET/P12X3C3D；對重組質(zhì)粒進行序列測定欧美色综合天天综合高清网、分析99这里还有精品，并將重組質(zhì)粒分別轉(zhuǎn)染BHK-21細胞，通過雙抗體夾心ELISA方法和間接免疫熒光標記方法檢測細胞中FMDV抗原的表達黄片ah视频，用電子顯微鏡觀察病毒空衣殼的組裝欧美一级人与嘼视频免费；為評價重組質(zhì)粒作為DNA疫苗對實驗動物及本動物的免疫效果，將重組質(zhì)粒經(jīng)肌肉注射方法接種豚鼠中文字幕第页日，并與酵母表達的純化FMDV China99株3D蛋白及帶有牛α干擾素的真核表達質(zhì)粒pcDNA3.1/IFN分別/同時免疫欧美精品AAAAAAAAA片，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；隨后將質(zhì)粒pcDNA3.1/P12X3Cwwwxxxx黄色、pcDNA3.1/P12X3C3D與帶有牛α干擾素的真核表達質(zhì)粒pcDNA3.1/IFN同時免疫牛xxxx欧美图片，三周后經(jīng)牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

為研制帶有O型口蹄疫病毒(Foot-and-Mouth Disease Virus国产黄色无码，F(xiàn)MDV)China99株結構蛋白基因及多個非結構蛋白基因的DNA疫曲人人操人人色人人干人人舔，本研究通過定點突變方法和PCR擴增方法，獲得包含有FMDV China99株結構蛋白P1国产精品对白交换、非結構蛋白2A屌屄黄色视频、3C以及部分2B国产特级毛片无码专区、3B編碼基因的片段P12X3C和包含有FMDV China99株結構蛋白P1亚洲爆乳AAA无码专区、非結構蛋白2A、3C欧美老妇乱子BBBBBBBW、3D以及部分2B精品射、3B編碼基因的片段P12X3C3D，將獲得的基因片段直接／酶切后與同樣處理的真核表達質(zhì)粒連接在线视频亚洲，分別得到重組質(zhì)粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D亚洲一级av一级无码毛片、pTARGET／P12X3C3D永久免费AV无码动漫网站在线观看；對重組質(zhì)粒進行序列測定、分析免费黄色视频醉网址，并將重組質(zhì)粒分別轉(zhuǎn)染BHK-21細胞免费看a在线，通過雙抗體夾心ELISA方法和間接免疫熒光標記方法檢測細胞中FMDV抗原的表達，用電子顯微鏡觀察病毒空衣殼的組裝国内自拍AV在线；為評價重組質(zhì)粒作為DNA疫苗對實驗動物及本動物的免疫效果91九色偷情自拍视频，將重組質(zhì)粒經(jīng)肌肉注射方法接種豚鼠，并與酵母表達的純化FMDV China99株3D蛋白及帶有牛α干擾素的真核表達質(zhì)粒pcDNA3.1／IFN分別／同時免疫97国产精品一区在线播放，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：隨后將質(zhì)粒pcDNA3.1／P12X3C免费看污网站十八禁永久免费、pcDNA3.1／P12X3C3D與帶有牛α干擾素的真核表達質(zhì)粒pcDNA3.1／IFN同時免疫牛，三周后經(jīng)牛舌皮攻以10~4ID_(50)的O型FMDV China99株免费黄片wwwwww。

assay foot：分析英尺

assay bar ==> 檢定金,鑒定棒 | assay foot ==> 分析英尺 | assay furnace ==> 試金爐