In order to ensure sensitivity and precision of transducer of spring beam,according to "stress concentration" design philosophy,based on reasonable choice of transducer,its strain and strength are calculated using FEM,the paster place of spring beam should be ascertained in reason,and the transducer's maximum strain orientation and measuring range are determined theoretically.

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According to the structure characteristics of L-B composite brake beam, the finite element model of L-B brake beam based on contact is established, and the stress distribution of the brake beam in different conditions calculated is using I-DEAS software. Calculated results show that the stress concentration mainly generates in the root of brake header slider, which fit with the faults in actual operation, and provide reliable basis to improve design and optimization.

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Under the excitation of 488 nm light beam of argon ion laser source, single suprahelical species could be observed with the aid of a common microscope due to the strong scattered light emitted by the suprahelical species. By capturing the RLS images of the single suprahelical species with a cooled charge coupled device camera, and analyzing the RLS data, herein an RLS imaging method of DNA was proposed based on the linear relationship between the counts of suprahelical species in the detection focus plane and the concentration of DNA in nanograms.

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