Methods we build rat retinal cells and rgcs cultured in virto by high pressure.put 80 mmhg pressure to rgcs in close tightly culture bottle for four hours, then collect rgcs and check the sum of apoptosis cells by flow cytometry.put the pressure 80 mmhg to rgcs in close tightly culture bottle for four hours,then collect rgcs and look for the apoptotic gene by gene chip.

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METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.

在差速貼壁+Thy1.1抗體及補(bǔ)體純化法的基礎(chǔ)上男人天堂网站在线，剪開大鼠顱骨国产偷在线视频网，顯露位于顱腔前方的嗅球，取出2只嗅球白丝美女很黄很黄A片，在顯微鏡下去除嗅球表面的軟腦膜和毛細(xì)血管及外周組織x9x9x9av，保留富含嗅鞘細(xì)胞的嗅神經(jīng)層和嗅球顆粒層，剪成1 mm3小塊分離獲取單細(xì)胞懸浮液在线国产www色，調(diào)整細(xì)胞密度至1×107 L-1caopeng在线视，接種在用poly-l-lysine包被的培養(yǎng)瓶或培養(yǎng)板中，于37 ℃亚洲一区欧美二区、體積分?jǐn)?shù)為5%的CO2培養(yǎng)箱中培養(yǎng)黄色视频在线aaaaaa，第3天用無血清DMEM/F12培養(yǎng)基換液培養(yǎng)。

METHODS: The rib trabeculae were resected and broken, trypsinizated and washed completely by PBS. Bone surface and non-adhesive floating cells in cleaning fluid were observed with inverted microscope. Rib trabeculae was washed by DMEM culture medium once, and cultured in culture bottle. The culture liquid was replaced by new one once a week. The osteoblast was moved from the sclerite a week later. The cells were fused monolayer and could be subcultured 4 to 6 weeks later.

切下人肋骨骨小梁剪碎美女张开腿让男人桶到底免费网站，胰酶消化后以PBS徹底沖洗直至于倒置顯微鏡下觀察骨片表面及清洗液中無粘著及漂浮的細(xì)胞红牛影视AV在线地址，以DMEM完全培養(yǎng)液清洗骨小梁1次，移入培養(yǎng)瓶內(nèi)培養(yǎng)精品国内在线，每周更換培養(yǎng)液1次，1周后可見成骨細(xì)胞自骨片移出，4~6周細(xì)胞融合成單層可作傳代培養(yǎng)精品欧美一区二区在线观看视频性色。

BOTTLE DOOLS：瓶中娃娃

9.絨條娃娃 BRAID DOOLS | 10.瓶中娃娃 BOTTLE DOOLS | 11.民俗娃娃 CULTURE DOOLS

CULTURE DOOLS：民俗娃娃

10.瓶中娃娃 BOTTLE DOOLS | 11.民俗娃娃 CULTURE DOOLS | 12.發(fā)條娃娃 DRIVING DOOLS

rotate tube culture：旋轉(zhuǎn)管培養(yǎng)

玻璃珠培養(yǎng)[系統(tǒng)] glass bead culture system | 旋轉(zhuǎn)管培養(yǎng) rotate tube culture | 滾瓶培養(yǎng) roller bottle culture