The innovation of the thesis rest with the selectiveness of a special enterprise, China Telecom, and then the article researches the main problems for its construction from an angle of view of the advance and promotion which enterprise culture made to a core competitiveness of the enterprise. Further more, the conclusion gives the key point and principia for a further construction.

本文的創(chuàng)新之處在于選擇了在中國(guó)電信行業(yè)中具有特殊地位的中國(guó)電信作為研究對(duì)象，從企業(yè)文化對(duì)企業(yè)核心競(jìng)爭(zhēng)力的提升和促進(jìn)作用入手自拍偷拍av网址，研究了中國(guó)電信企業(yè)文化建設(shè)中存在的主要問題并給出了企業(yè)文化進(jìn)一步建設(shè)的要點(diǎn)和關(guān)鍵原則。

I would love nothing more than to continue my studies in this field.

Further more，我非常熱愛我的專業(yè)中日韩最新无码视频网站大全，相信在這一領(lǐng)域繼續(xù)學(xué)習(xí)，研究欧美一级做一级A片视频大鸡巴破处，是一件非撑访?8免进？鞓返氖虑?/p>

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

結(jié)果 成功構(gòu)建突變?nèi)薈D59的真核細(xì)胞表達(dá)系統(tǒng)：運(yùn)用陽離子脂質(zhì)體介導(dǎo)法將含有突變?nèi)薈D59的PALTER—MAX重組質(zhì)粒與PCDNA共轉(zhuǎn)染入CHO細(xì)胞：用含有400ug／mlG418的F12培養(yǎng)基培養(yǎng)14天，篩選出穩(wěn)定陽性表達(dá)克隆麻豆国产原创视频在线，RPMI1640培養(yǎng)基擴(kuò)增獲得穩(wěn)定表達(dá)細(xì)胞株变态自拍日韩欧美在线观看网站视频，并用流式細(xì)胞術(shù)進(jìn)一步篩選出高效表達(dá)細(xì)胞株分別命名為PALTER—CD59—CH01、PALTER—CD59—CH02亚洲一级这片，表達(dá)率分別為53.7％日韩性插、54.5％；應(yīng)用免疫組化方法国产一级一级A一级A级、免疫熒光技術(shù)進(jìn)一步鑒定陽性細(xì)胞株怕怕怕18成禁人视频免费；RPMI1640培養(yǎng)基大量擴(kuò)增PALTER—CD59—CH01、PALTER—CD59—CH02細(xì)胞株黄色网址在线免费，裂解細(xì)胞得到CD59蛋白質(zhì)久久精品A片777777；通過SDS—PAGE凝膠電泳技術(shù)、免疫印跡技術(shù)日韩欧清无码激情性视频、固相酶聯(lián)免疫吸附試驗(yàn)驗(yàn)證了這兩中文摘要個(gè)陽性細(xì)胞株CO59蛋白的高效表達(dá)一级特黄特色的免费网站；50mM核糖培養(yǎng)72小時(shí)，獲得突變?nèi)薈D59糖化細(xì)胞株www.jjzz日本视频，BCECF染料釋放試驗(yàn)結(jié)果顯示黄片视频2019，PALTER一CD59一CHOI、pALTER一CD59一CHOZ細(xì)胞較PALTER一CHO細(xì)胞染料釋放率低尤物网站视频免费看，未糖化PALTER一CD59一CHOI人人操人人操操、PALTER一CD59一CHOZ細(xì)胞比較糖化后細(xì)胞染料釋放率低。

add: to say something more; to make a further remark：附加說明

acknowledge: To disclose something that has been concealed. 承認(rèn) | add: to say something more; to make a further remark 附加說明 | admit: To disclose something reluctantly. 承認(rèn)

more important：遞進(jìn)

1免费网站在线观看人成视频、轉(zhuǎn)換:however, nevertheless | 2、遞進(jìn):more over, further (more), even worse, more important | 3久久riav.、因果:for this reason, for the same reason, consequently

v More and More Students Have Mobile Phones：移動(dòng)電話在學(xué)生中的

v Can Computers Replace Paper and Pen?-電腦能代替紙筆嗎? | v More and More Students Have Mobile Phones-移動(dòng)電話在學(xué)生中的 | v Further Study in Foreign Countries-出國(guó)深造