The EBs were transferred to gelatin-coated Petri-dish and cultured in differentiation medium 1 for 6 days, and then change to differentiation medium 2 for another 6 days.

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CD34+ cells were harvested from G-CSF mobilized adult blood and umbilical cord blood and incubated onto fibronectin/gelatin-coated Petric dishes in the presence of recombinant human vascular endothelial cell growth factorand basic fibroblast growth factor.

毛寧為證明出生后血液循環(huán)中存在內皮細胞的前體細胞，從G-CSF動員的成人外周血及臍帶血中分離CD34+細胞不卡一区二区在线观看免费，將之接種于纖連蛋白與明膠鋪板的培養(yǎng)皿上高清一区二区三区免费杂交，培養(yǎng)體系中加入重組人內皮細胞生長因子和重組人堿性成纖維細胞生長因子。

The aggregates cultured on N-cad-Fc-coated dishes compared with gelatin-coated dishes, the expression of cardiac-specific genes cardiac actin and gata-4 were much higher, but the expression on 10 mg/L N-cad-Fc-coated dishes was slightly low than on 2.5 mg/L N-cad-Fc-coated.

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After γ ray treatment, two kinds of cells were incubated on 6-well gelatin-coated plate with density of 2.5×104/cm3. hESCs were cultured on HAFi or MEF feeder cells containing β-mercaptoethanol DMEM/F12 and basic fibroblast growth factor.

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On gelatin-coated surface, cells were more resistant to shear flow compared to those on tissue culture polystyrene or biocompatible polyurethane. The newly grown cells on the gelatin substrate after flushing for a period of time became further resistant to shear flow.

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