MethodsCSF was extracted from patients with GBS and controls, and proteins were precipitated with icecold acetone. Isoelectric focusing electrophoresis was employed as the first dimension and sodium dodecylsulfatepolyacrylamide gel electrophoresis was employed as the second dimension.

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icecold：冰冷

icecloudcrystal冰晶云 | icecold冰冷 | icecolorprocess冰染法