The optimum initial substrate concentration for cultivation of inoculum was 8%.The optimum conditions for batch fermentation were 0.04vvm of air flow,5.8 of pH value.The fed-batch fermentation showed that the productivity of Klebsiella pneumoniae CICC 10011 was better than K.pneumoniae CGMCC 1206.The maximum concentration of 2,3-butanediol and acetoin was 103.38 g/L (90.98 g/L of 2,3-butanediol),and the yield reached 82.2%of its theoretical value.The fermentation conditions for K.pneumoniae.

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Animal studies included in the attached Material Safety Data Sheet showed AFT to be nonirritating to either abraded or intact skin and dust inhalation studies with mice failed to produce any fatalities at the maximum achievable concentration of 150 mg/m3 about 20 ppm in air.

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According to the energy conservation theory, BOM and CSIM4 were coupled. The BOM has no treatment on transmission solar radiation, which is of great importance when the model is adapted to Arctic Ocean. So the treatment was introduced to BOM. Through numerical test on different lead albedos, it was found that sea ice thickness is not so sensitive to lead albedo, which may be contribute to the lead occupies little ratio within multiyear sea ice pack. The reason of summer over-melt of arctic sea ice is the NCEP reanalysis downward solar radiation being larger than its reality. Then the arctic sea ice climate variability was simulated. Results showed that: simulated ice thickness change is in accord with the submarine investigated mean sea-ice draft changes. Simulated annually maximum ice thickness along the Eurasian continental oceans are closely related to the observed ones. The long-term mean simulated ice motion has the same features of the SSM/I derived ice motion. Sea ice extents in differential sub-regions have same trends comparing to the satellite passive-microwave data derived ones. Simulated ice concentration is closely related to the observed in the Arctic sub-regions. Sea ice flux through the Fram Strait involves ice concentration, motion and thickness. It is a composite criterion for sea ice model evaluation. The simulated ice area and volume export through the strait accord with the satellite derived or statistically reconstructed ones.(5) The simulated ice thickness climate variability and mean sea surface current of the coupled model were analyzed, results showed: the total ice volume in the Arctic Ocean has a significant decreasing trend. The volume variability is of a 10-year timescale oscillation, with two major periods of 12-13a and 18-20a. Mean ice thickness in the arctic sub-seas has different tendencies. It has an increasing trend in the Barents-Kara Sea and Baffin Bay-Labrador Sea, and decreasing in the others. The characteristic time scale of 7-10a wherein the river discharges leads the Fram Strait ice volume export is about the period that river water takes to be conveyed across the Arctic Ocean.(6) Using the simulated ice distribution in the Arctic Ocean and China precipitation, air temperature and SST in tropical key regions, the climate teleconnection were studied. Result showed: When the mean sea ice thickness is large in the central Arctic Ocean and Chukchi-Beaufort Sea , and small in the Barents-Kara Sea and Baffin Bay-Labrador Sea , the precipitation in South China, Tibetan Plateau, and the north part of Northeastern China are always smaller than normal, and v. v. When the mean ice thickness is small in CA, BC, East Siberian Sea and Greenland-Iceland-Norwegian Sea , and large in BL, The air temperature in north-eastern China, the southern of Tibetan Plateau, and Hainan Island, are always lower than normal, and v. v. In addition, when the sea ice is thick in BC and BL, the SST is larger in the middle and eastern Pacific Ocean, and is smaller in the tropical Southeastern Indian Ocean.

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