Objective To investigate the effect of different splitting solutions and decompaction treatment on the bisection of mouse embryos at morulae and blastocysts stage and their subsequent transfer after cultured.

目的 研究不同分割液和分割前胚胎去致密化與否對(duì)昆明白系小鼠的桑椹胚和囊胚的徒手分割以及分割后胚胎移植的影響欧美乱大交免费。

By statistic analysis, there were significant differences with the average cell number of each morulae, early blastula and blastula between in vivo and in vitro, there were no differences with the hatching of blastula between in vivo and in vitro.

經(jīng)統(tǒng)計(jì)分析體內(nèi)桑椹胚h网站在线看免费、早期囊胚美女扒开腿让男人桶爽动漫、囊胚的每一胚胎平均細(xì)胞數(shù)與體外桑椹胚国产高清老太婆一级AA片、早期囊胚、囊胚的每一胚胎平均細(xì)胞數(shù)有顯著差異免费看黄网站在线观看免费最新；擴(kuò)張囊胚的體內(nèi)天天透天天狠狠狠的，體外每一胚胎平均細(xì)胞數(shù)沒有顯著差異。

Studies on mouse nuclear transfer with compacted morula blastomeres as donors In this study, C57BL/6 mouse morulae blastomeres and Kunming white mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different sucrose concentration on the manipulation time of nuclear transfer, and the in vitro development of reconstructed embryos.

以致密桑椹胚卵裂球?yàn)楣w克隆小鼠的研究該研究以C57BL/6 小鼠致密桑椹胚卵裂球?yàn)楣w在线观看美女一区、昆明白小鼠MⅡ期卵母細(xì)胞為受體亚州一级特黄片免费视频在线，研究了不同蔗糖濃度對(duì)核移植操作時(shí)間及重構(gòu)胚體外發(fā)育的影響。

Addition of 3μg/ml CB to CM containing 0.625μg/ml CHX increased the proportion of oocytes with two pronuclei (38. 9% vs 15. 1%, P＜0.05) and developed into morulae/blastocysts (40.7% vs 26. 8%, P＜0.05), but did not affect the percentage of oocytes cleaved and total pronucleus rate.

如果在含有0.625μg/ml CHX中添加3μg/ml CB国产公开免费视频，可明顯提高雙原核的形成率（38.9％vs 15.1％一特级黄片视频，P＜0.05）和桑椹胚/囊胚發(fā)育率（40.7％vs 26.8％，P＜0.05）免费性爱黄色视频，但對(duì)其激活分裂率和總原核形成率無(wú)明顯影響（P＞0.05）免费观看黄网站国产。

The best survuval rate of each stage thawed-embryos existed in groups of Zygote EDT3O-two-step method with I mm equilibration(66%), of Morulae EDT3O-two-step method with I mm equilibration(94%) and EDT4O-two--step method with 0.5 mm equilibration(94%), of Blastocyte EDT3O-two-step method with I mm equilibration(91%) and EDT4O-one-step method with I mm equilibration(91%).

總之，我們的研究表明操x网站，DET玻璃液冷凍方法是簡(jiǎn)單强伦姧人妻一区二区、經(jīng)濟(jì)而又高效的，冷凍小鼠各階段胚胎都能獲得較高的存活率黄片在线免费看A片，其中受精卵的EDT30二步法1分鐘平衡組(66％)免费无码高精品视频色欲、桑椹胚的EDT30二步法1分鐘平衡組(94％)和EDT40二步法0.5分鐘平衡組(94％)、囊胚的EDT30二步法1分鐘平衡組(91％)和EDT40一步法1分鐘平衡組(91％)冷凍效果最好日本一区二区在线播放。

morulae：(復(fù)數(shù))桑椹胚

morula 桑椹胚 | morulae (復(fù)數(shù))桑椹胚 | mosaic 鑲嵌物;鑲嵌型;花葉的