In the absence of appropriate costimulation, partial activation of T cells can be lead to a state of long-lasting antigen-specific unresponsiveness or anergy.

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The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

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Results: The proliferation of Kasumi-l cell was obviously inhibited by VPA on dose- and time-dependent manner. The IC50 was 2.33 mmol/L treated with VPA for 72 h. Incubation with VPA, the cell ratio at G0/C1 phase increased. VPA induced myeloid cell differentiation antigen CD11b to increase with a time-and dose-dependent way and caused apoptosis. Wright's staining and light microscope were used to show the morphology of partial differentiation and obvious apoptosis with 3 mmol/L VPA for 72 hours. The typical DNA ladder of apoptosis characteristic was observed with DNA gel electrophoresis.

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