To study the cytopathogenic effect of mycoplasma membrane.the pro- toplasmic membrane of the mycoplasma causing swine enzootic pneumo- nia was prepared by the repeated Freeze-thaw method or the osmotic shock method,and was then inoculated into the rabbit kidney primary tissue cultures for examining their biological activities.The experimental results were as follows:in the cultures inoculated with 25～50μg of membrane protein ,the cytopathogenic effect was considerable.in the cul- tures inoculated with ...

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