METHODS: Neonatal mouse brain tissues were sterilely obtained to prepare monoplast suspension.

無菌條件下取新生小鼠腦組織又长又粗黄色视频，制備單細胞懸液。

METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.

無菌狀態(tài)下取出胎兒肝臟白白视频在线，以膠原酶消化法和機械分離法獲得胎兒肝臟來源單細胞懸液操b校园高清无码，分別采用1.070 g/mL Percoll分離液和1.077 g/mL Ficoll分離液進行密度梯度離心，吸取界層細胞黑人大战白妞系列AV，添加含體積分數(shù)為0.1 FBS一级片操逼视频、20 μg/L肝細胞生長因子、40 μg/L表皮生長因子的DMEM/F12新鮮培養(yǎng)基誘導9 d午夜欧美一区二区。

ML of bone marrow was sterilely aspirated from the posterosuperior iliac spine of 36 healthy mongrel dogs. BMSCs were isolated and purified by Percoll density gradient centrifugation and cultured in vitro by adherence method.

選擇健康雜種犬36只99riAV.cn，無菌抽取犬骨髓液10 mL，經(jīng)Percoll液密度梯度離心后貼壁法體外培養(yǎng)骨髓間充質(zhì)干細胞麻豆国产原创视频在线，傳2代后行5-氮胞苷誘導变态自拍日韩欧美在线观看网站视频，5-溴脫氧尿苷標記備用。

All patients received a massive bone allograft that had been sterilely procured without secondary irradiation.

所有的病人都接受了經(jīng)消毒而未進行二次輻射的大塊同種異體骨亚洲一级这片。

METHODS: Bone marrow was sterilely isolated. BMSCs were isolated and purified by the density gradient centrifugation.

無菌條件下抽取豬骨髓液日韩性插，密度梯度離心法分離純化骨髓間充質(zhì)干細胞。