Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭質(zhì)粒YCEp1為載體構(gòu)建西方許旺酵母部分基因組文庫羞羞事网站免费进入，在大腸桿菌中擴(kuò)增后提取混合質(zhì)粒DNA可以免费看黄色视频的视频的视频，經(jīng)電轉(zhuǎn)化非缺陷標(biāo)志酒精酵母 AS.2.1364久久r久久，在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力篩選含水解淀粉的陽性轉(zhuǎn)化子黄色视频了88，從陽性轉(zhuǎn)化子中分離重組質(zhì)粒證實(shí)含5.0kb的插入片段国产精品久久九九免费视频，用α-淀粉酶基因兩端序列設(shè)計(jì)的引物PCR擴(kuò)增及擴(kuò)增片段序列分析證實(shí)該片段中含有α-淀粉酶全部編碼序列免费看的国产区视频。

To study the applicable prospect of suicide gene on tumor therapy in clinic,we cloned the gene of D-amino acid oxidase and the gene of yeast cytosine deaminase.We hope to establish the transgenic mices of DAAO gene and YCD gene,and the two transgenic strain mices are valuable animal system for studying the biological characteristic of DAAO gene and YCD gene ,for studing the killing activity of DAAO/D-Ala and YCD/5-FC gene therapy systems on tumor.The D-amino acid oxidase gene derived from R.gracilis and it could oxidize D-amino acids. Hydrogen peroxide(H2O2) is a reactive oxygen species generated in the deamination of D-Ala catalyzed by DAAO.

為了探討自殺基因在臨床腫瘤治療中的應(yīng)用前景，建立自殺基因腫瘤治療評價(jià)的動(dòng)物模型亚洲v欧美v日韩Ⅴ中文字幕，我們克隆了D-氨基酸氧化酶(D-amino acid oxidase波多野结衣性爱视频，DAAO)基因和酵母菌胞嘧啶脫氨酶(yeast cytosine deaminase2020精品极品国产色在观看，YCD)基因，希望建立DAAO基因及YCD基因轉(zhuǎn)基因小鼠舔美女屁眼的视频一区二区，為研究DAAO及YCD基因的生物學(xué)特性Xxxxxav视频、開發(fā)和評價(jià)DAAO／D-Ala及YCD／5-FC自殺基因系統(tǒng)進(jìn)行腫瘤治療建立良好的實(shí)驗(yàn)動(dòng)物模型。

Nine medium compositions were used to study their effect on I40 using Plackett-Burman design. The production of biomass for I40 was affected in order of yeast extract, tryptone, glucose, and production of protease affected in order of feather, tryptone and yeast extract.

最是化條件探討方面xigouqi.com，於基礎(chǔ)培養(yǎng)基中分別添加九種單因子黄色视频免费二级毛片，以PB design分析，結(jié)果發(fā)現(xiàn)對於I40生長影響較為顯著的因子依序?yàn)椋簓east extract黄页在线观看网站、tryptone久久精品视频在现、glucose，而對於該菌生產(chǎn)蛋白酶產(chǎn)量影響較為顯著的因子依序?yàn)椋篺eather看看国产黄片视频在线、tryptone午夜男女免费观看视频、yeast extract。

yeast-bitten：發(fā)酵過度的

isostemonidine 異百部定 | yeast-bitten 發(fā)酵過度的 | silver-plated 包銀的, 鍍銀的

yeast-bitten：發(fā)酵過度

yeast 酵 | yeast-bitten 發(fā)酵過度 | yeastiness 起泡