Olive oil, tributyrin and p-nitrophenyl palmitate were used as substrates to measure lipase activity.

分別以橄欖油91AV免费视频、三丁酸甘油酯和對(duì)硝基苯酚棕櫚酸酯為底物檢測(cè)展示的脂肪酶酶活精品视频9999。

Eight lipase-producing bacterium strains were screened from 40 cold-adapted strains, these strains isolated from the mud of Parece Vela sea basin in the Pacific Ocean of 5010m deep, of which two similar strains have the highest lipolytic activity and are positive to olive oil.

通過(guò)三丁酸甘油酯平板法從太平洋帕里西維拉海盆5010m的底泥中共篩出八株可產(chǎn)脂肪酶的菌株情欲网址，其中的兩株生理生化特征極其相近的菌脂肪酶活性最高伊人久久综合精品永久图片，并且對(duì)橄欖油平板產(chǎn)生熒光久久99精品国产免费。

Objective To investigate the differentiation of NB4 and MR2 cells induced by tributyrin in combination with different doses of all-trans retinoic acid and to see if TB can induce apoptosis of NB4 and MR2 cells.

目的 研究三丁酸甘油酯與全反式維甲酸不同濃度組合對(duì)NB4及MR2細(xì)胞的誘導(dǎo)分化作用，并觀(guān)察TB對(duì)NB4av九一、MR2細(xì)胞是否具有促凋亡作用欧洲人与动牲交Α欧美精品。

The most common methods used for screening of lipase-producing strains are observation of transparent circle on tributyrin agar plate and color-changing circle on olive oil agar plate.

在脂肪酶產(chǎn)生菌的篩選中，最常用的方法是三丁酸甘油酯瓊脂平板透明圈法和橄欖油瓊脂平板變色圈法亚洲欧美中文日韩V日本。

However, the recombinant yeast could not grow on the traditional tributyrin plate because this kind of medium is lack of essential nutrients for yeast growth.

而傳統(tǒng)的三丁酸甘油酯平板缺乏有效營(yíng)養(yǎng)成分日韩性色生活免费观看，重組酵母無(wú)法生長(zhǎng)；橄欖油平板靈敏度較低大伊人一区在线视频，低脂肪酶活力的重組酵母不能產(chǎn)生變色圈日本人人操人人摸在线，這給產(chǎn)脂肪酶基因工程菌的鑒定帶來(lái)了困難。

We use plate to check the lipase activity ,the lipase activity is very low, Compared to the wild type , the mutant could not form the clear halo on the olive oil plate but tributyrin plate, it showed that the 227 th Serine was essential for the lipase activity.

與野生型相比女秘书的黄色大片，突變體脂肪酶的酶活很低观看A片的网址，不能在橄欖油平板上形成透明圈，但可以在以三丁酸甘油酯為底物的平板上形成透明圈摸摸日日，表明第227位絲氨酸可能對(duì)脂肪酶酶酶活具有重要影響无码毛片视频一区二区无码。

To elucidate the possible mechanism of differentiation and /or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor, the level of acetylated histone H3 was detected by Western blot, p21~ WAF1expression was detected by semi-quantitative RT-PCR.

為了探討組蛋白去乙酰化酶抑制劑三丁酸甘油酯(tributyrin2021国产精品免费观看，TB)誘導(dǎo)NB4激情精品一区二区在线观看、K562白血病細(xì)胞分化和凋亡的作用機(jī)制欧美一级A片黑人又粗又大，利用Westernblot方法及逆轉(zhuǎn)錄聚合酶鏈反應(yīng)檢測(cè)TB作用前后NB4、K562細(xì)胞組蛋白H3乙跫忧诒仁悠担化水平以及p21WAF1表達(dá)量的改變欧美另类XXXXHD高清。

To elucidate the possible mechanism of differentiation and/or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor , the level of acetylated histone H3 was detected by Western blot, p21(superscript WAF1) expression was detected by semi-quantitative RT-PCR.

為了探討組蛋白去乙酰化酶抑制劑三丁酸甘油酯(tributyrin最新国产小视频， TB)誘導(dǎo)NB4精品99r、K562白血病細(xì)胞分化和凋亡的作用機(jī)制，利用Western blot方法及逆轉(zhuǎn)錄聚合酶鏈反應(yīng)檢測(cè)TB作用前后NB4a∨在线视频、K562細(xì)胞組蛋白H3乙?7人人草人人；揭约皃21(上標(biāo) WAF1)表達(dá)量的改變。

The activity of lipasc from S78- pVT102U/a -lip was up to 20U/ml after 72h fermentation; The product of S78-pVT102U/a-L-lip07 was little, only formed the halo on the tributyrin plate.

其中日韩精品导航大全视频在线播放免费，重組酵母S78-pVT102U／α-lip搖瓶發(fā)酵72h肏屄在线免费视频，酶活可達(dá)20U／ml；S78-pVT102U／α-L-lip07表達(dá)的脂肪酶很少嗯一毛片黄片操逼片，表達(dá)產(chǎn)物僅在三丁酸甘油酯平板上形成透明圈一av天堂。

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

該菌株經(jīng)IPTG誘導(dǎo)可表達(dá)分子量約80 kDa的融合蛋白，SDS-PAGE分析表明融合蛋白位于菌體超聲裂解后的上清中81色色色，用蛋白純化試劑盒HisTrap~ HP純化后得到單一的目的蛋白色色很污的视频网站，約80 kDa，且純化的融合蛋白具有脂酶活性久久免费美女黄片视频，可以分解三丁酸甘油酯www.一级毛片。

butyrin：甘油三丁酸酯;三丁醯甘油

"酪酸;丁酸","butyric acid; butanoic acid" | "甘油三丁酸酯;三丁醯甘油","butyrin" | "丁胺酸","butyrine"

butyrin：三丁酸甘油酯;乳脂甘油酯

butyricacidfermentation丁酸發(fā)酵 | butyricin丁酸梭菌素 | butyrin三丁酸甘油酯;乳脂甘油酯

tributyrin, glyceryl tributyrate, butyrin：甘油三丁酸酯,三丁酰甘油

"擬榖盜屬","Tribolium" | "甘油三丁酸酯,三丁醯甘油","tributyrin, glyceryl tributyrate, butyrin" | "磷酸[三]鈣","tricalcium phosphate, tribasic calcium phosphate"

butyrin,tributyrin,glyceryl tributyrate：甘油三丁酸酯,三丁酰甘油

西酸酸敗 butyric rancidity | 甘油三丁酸酯,三丁酰甘油 butyrin,tributyrin,glyceryl tributyrate | 丁胺酸 butyrine

butyrinase：丁三酸甘油酯酶 脂羥化酶

butyrin 酪脂 | butyrinase 丁三酸甘油酯酶 脂羥化酶 | butyroidtumor 黃油樣腫脹

butyrinase：丁三酸甘油酯酶

butyric | 奶油的, 由奶油中提取的, 酪酸的 | butyrinase | 丁三酸甘油酯酶 | butyrobetaine | 丁內(nèi)銨鹽 三甲氨基丁內(nèi)鹽

trimyristin; Myristin：三肉豆蔻酸甘油酯

氫氧化三甲基乙烯基銨;神經(jīng)堿 trimethylvinylammonium hydroxide; neurine | 三肉豆蔻酸甘油酯 trimyristin; Myristin | 正三丁硼[烷] tri-n-butylborine

Tributyrin：三丁酸甘油酯

經(jīng)由十二種油的篩選,發(fā)現(xiàn)三丁酸甘油酯(tributyrin)以及己酸乙酯(ethyl caporate)對(duì)於紫杉醇以及喜樹(shù)堿的溶解度最高. 由於希望系統(tǒng)能形成在盤(pán)尼西林(penicillin)發(fā)現(xiàn)以及應(yīng)用之后,相較於其他所有種癌癥協(xié)會(huì)(NCI)針對(duì)許多植物進(jìn)行藥物的篩選,

Tributyrin：三丁精 三丁酸甘油酯

silicide resistor 硅化物電阻器 | tributyrin 三丁精 三丁酸甘油酯 | summation of contraction 收縮疊加[總和]

Tributyrin：甘油三丁酸酯

三丁胺 tributylamine | 甘油三丁酸酯 tributyrin | 甘油三癸酸酯 tricaprin