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The differentiation coefficient is 0.07 between three inbreed families respectively. The highest level of genetic homozygosity is in families Ⅱ at 18, but genetic variation is a little difference between inbreeding pig and mice. However, this study revealed the pattern of genetic variation at microsatellite loci in the 3 inbred families of WZSP with one of the most important findings to be the high level of unexpected heterozygosity at some loci, e.g. Sw874 and Sw936 that could be part of the unique genetics of three families. A hypothesis is that certain functional genes linked with such microsatellite loci may be involved in the maintenance of viability of these inbred families of WZSP remains for further investigation.

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The conversion boundary of self-incompatibility was researched by sowing by stage. Sunshine of day and temperature was studied critically by investigating leafage and researching traits indoors. The results showed that the daily minimum temperature was the principal factor of affinity conversion and the affinity conversion boundary was 16.5~18.1℃.HE97 was primarily regard as self-incompatible line in this research. The method was by inbreeding and sib HE97, Half the silk of HE97 and Zheng58. Inbreeding and cross have been done simultaneously. The extending condition of pollen tube was observed by dying the silk after pollinating 4 hours. The individuals with pollen tube were observed having seeds, while the individuals without pollen tube were observed having few seeds or no seeds. So this study regard HE97 as sporophytic self-incompatibility .The planting ration of female parent HE97 and male parent 87-1 was 4:1.The nature cross-pollination was studied by the theory of endosperm xenity, the study show that the setting percentage of HE97 was 625%, in which the seed in self was 70% and the seed in hybrid was 30%. This was consistent with the study of silk anatomy structure, then come to the same conclusion: compatible pollen germination and fertilization can stimulate the self-incompatible pollen germination and fertilization in the same corn cob.

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Purpuraristatus, the growth, fertility, chromosome configuration, EST isozyme etc. were analyzed in this paper. The results showed that the growth potentiality of (E. sibiricus×E. purpuraristatus) F2 and (E. purpuraristatus×E. sibiricus) F1 were much stronger than their parents, the plant height of the former was 143.2 cm. The whole plant was reseda, the latter was 129.7 cm. The spike nodding of two hybrids were in the middle of their parents, the anther was yellow; the pollen fertility was 0.02%~0.03%, seed set was 0; the average chromosome configuration of former pollen mother cell at PMC M Ⅰ was 6.90Ⅰ+14. 02Ⅱ, the latter was 7.82Ⅰ+13.59 Ⅱ, and lagging chromosome and bridge fragment were observed at meiosis anaphase Ⅰ the EST of the two hybrids F1 and their parents at tillering stage was some certain different in locus, number and intensity.

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We also detected genomic changes which were possibly associated with the reduced fitness in backcross by amplified fragment length polymorphism. The changes mainly involved loss of parental AFLP bands (backcross1, F1 hybrid female×olive flounder male, 3.96%; backcross2, F1 hybrid female×summer flounder male, 6.03%) and gaining of novel bands (backcross1, 5.63%; backcross2, 3.28%).

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In the three systems, system realizes the crosslinking of PVC bases on the peroxide crosslinking mechanism, the tensile strength of crosslinked PVC has been improved somewhat, the IR spectrum of gel demonstrates that there are characteristic absorption peaks of n-alcohol that belongs to ethanol amine and benzenoid group. From the analysis of gel structure , deduces that the mechanism of crosslinking reaction: under the initiations of peroxide and high temperature, PVC yields macromolecules free radicals, at the same time , styrene"s polymerization and grafting onto PVC takes place, at last the system forms crosslinking structure through coupling between radicals; confirms that triethanol amine can absorb HC1 produced by degradation of PVC, so improves the crosslinking efficiency of peroxide, decreases the HCl"s promotion for PVC"s degradation; deduces that triethanol amine can activate the PVC macromolecules and prolong the free radicals" life-time of PVC macromolecules; the effect to the PVC macromolecule makes styrenes graft reaction easy, and thus improves the crosslinking efficiency.

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