The results showed that potato genotypes affected callus and adventitious bud formation.

本研究對(duì)影響農(nóng)桿菌介導(dǎo)的馬鈴薯轉(zhuǎn)基因植林再生的基因型和激素兩個(gè)因素進(jìn)行了探討97大学生情侣酒店自拍亚洲精品。

Objective: To investigate the effect of calcium phosphate cement on IL-6 and TNF-α expression in rabbits.

目的：探討植入骨水泥不同時(shí)期對(duì)白細(xì)胞介素6(IL-6)，腫瘤壞死因子α應(yīng)激反應(yīng)蛋白表達(dá)的影響免费在线看黄色国产。

Tumefaciens mediated transformation using cotyledonary node as explants was optimized; 3 methods including cutting chapiter, ovary injection and chapiter spreading for pollen tube pathway was compared. Cutting chapiter and ovary injection can both achieve insect resistant gene tranformants.

優(yōu)化了以子葉節(jié)為外植體的農(nóng)桿菌介導(dǎo)轉(zhuǎn)化技術(shù)国产麻豆区精品系列在线；比較了切割柱頭、子房注射和子房涂抹3種花粉管通道法處理授粉后黃瓜子房的轉(zhuǎn)化效果日韩偷窥无码少妇AV，切割柱頭和子房注射均可成功獲得黃瓜抗蟲轉(zhuǎn)基因植株亚洲狠狠草视频。

The best optimum conditions of the Agrobacterium mediated genetic transfornation were that leaves explants were immersed in the diluter 10 times Agrobacterium bacilli at OD600 of 0.6 for 8 minutes after being pre-cultured for 2 days,and then co-cultivated for 3 days.Then these explants were transferred to selected and rooting medium which contained 50 mg/L Kanamycin and 500 mg/L Carbenicillin.According to the transformation system, 54 transgenic plants were initially screened,then after PCR analysis and RT-PCR analysis 25 transgenic plants were obtained.

根癌農(nóng)桿菌介導(dǎo)的芪合酶基因遺傳轉(zhuǎn)化的最佳條件為：將預(yù)培養(yǎng)2 d的煙草外植體，用稀釋10倍的GV3101菌株菌液（OD600=0.6）浸染8 min后午夜少妇性私人影院，共培養(yǎng)3 d99re视频在线这里，然后轉(zhuǎn)入含卡那霉素50 mg/L和羧芐青霉素500 mg/L的篩選分化培養(yǎng)基上誘導(dǎo)分化，待抗性芽長到2～3 cm后野花社区大全免费观看3超碰，轉(zhuǎn)入含Km 50 mg/L和Cb 500 mg/L的篩選生根培養(yǎng)基上進(jìn)行篩選国产精品区在线，在以上最佳遺傳轉(zhuǎn)化條件下，初步篩選到54株轉(zhuǎn)化體久久夜色精品，經(jīng)PCR和RT-PCR檢測獲得25株轉(zhuǎn)化煙草植株欧美精品-精品国内自产拍在线。

Seedling epicotyl segments of 'Xupu' sweet orange(Citrus sinensis L.Osb.)were used as explants to establish Agrobacterium-mediated transformation system with chit42 and phyB genes.

為改良柑桔品種，以普通溆浦甜橙實(shí)生苗上胚軸為外植體国产又黄又湿又色的免费网站，建立了農(nóng)桿菌介導(dǎo)的遺傳轉(zhuǎn)化體系欧洲无码在线免费，并把chit42基因和phyB基因?qū)肫渲小?/p>

The half-seed method system of Agrobacterium-mediated soybean transformation was researched applying healthy explants rates, resistant tufty shoots and elongated shoots as targets. Explants of soybean grow well in environments with abundant light and oxygen in cocultivation.

以農(nóng)桿菌介導(dǎo)的半種法大豆遺傳轉(zhuǎn)化系統(tǒng)為基礎(chǔ)，對(duì)目的基因進(jìn)行轉(zhuǎn)化欧美老妇交乱视频69式；以健康外植體獲得率亚洲日韩视频第一页、抗性叢生芽獲得率和抗性芽伸長比率為指標(biāo)，對(duì)轉(zhuǎn)化系統(tǒng)條件進(jìn)行優(yōu)化。

In this study, some transgenic cotton plants with a phytase gene were obtained successfully via agrobacterium-mediated method of embryogenic calli.

本研究采用農(nóng)桿菌介導(dǎo)的方法久久综合精品一区无码，將外源植酸酶基因?qū)朊藁ㄆ贩N的胚性愈傷組織中a片一区二区白嫩美女，經(jīng)培養(yǎng)獲得再生植株。

A modified Galanthus nivalis agglutinin gene was introduced into the genome of main cultivar 'Ningqi No.1' of Lycium bararum in vitro with explants of leaf disks mediated by Agrobacterium tumefaciens. Several factors affecting transformation efficiencies were studied based on induction rate of resistant callus. An effective system for genetic transformation of Lycium bararum was set up, and 70 kanamycinresistant transformants were obtained.

為了解決枸杞蚜蟲侵害的難題国产免费AⅤ片在线观看播，以寧夏枸杞主栽品種&寧杞1號(hào)&葉片為外植體人人操人人射人人干，通過農(nóng)桿菌介導(dǎo)的轉(zhuǎn)化方法，將改造后的雪花蓮凝集素GNA基因轉(zhuǎn)入枸杞日韩小视频在线，以抗性愈傷組織誘導(dǎo)率作為轉(zhuǎn)化效率的指標(biāo)国语对白露脸刺激自拍，對(duì)影響轉(zhuǎn)化效率的因素進(jìn)行了研究，初步建立了轉(zhuǎn)化頻率較高的轉(zhuǎn)化系統(tǒng)亚洲日本网。

Agrobacterium tumefaciens-mediated transformation system of Medicago truncatula cotyledonary-node explants Abstract The three-day-old seedlings of Medicago truncatula, which geminated in medium containing up to 5mg/L 6-BA, were used to prepare the cotyledonary-node explants which contained one cotyledon and 1-2mm of split hypocotyl. High-efficiency regeneration system of Medicago truncarula was developed by axillary shoot organogenesis of cotyledonary-node explants.

論文第二部分 Medicago truncatula遺傳轉(zhuǎn)化和植株再生體系的研究第一章根癌農(nóng)桿菌介導(dǎo)的Medicago truncatula子葉節(jié)外植體轉(zhuǎn)化系統(tǒng)的研究本文利用在高達(dá)5mg／L 6-BA的萌發(fā)培養(yǎng)基上生長3天的Medicago truncatula幼苗三级片黄片日韩，制備獲得由一片子葉和1-2mm切分的胚軸組成的子葉節(jié)外植體。

Inoculating test plants through vector,observing symptom and using universal primers(R16mF2/R16mR1)for phytoplasma 16S rDNA were conducted.1.4 kb DNA fragments were amplified by polymerase chain reactionin DNA samples extracted from inoculated wheat and viruliferous Psammotettix striatus L.respectively.

采用介體接種植物久久阿只有精品，癥狀觀察和應(yīng)用植原體16S rDNA基因通用引物對(duì)R16mF2/R16mR1進(jìn)行PCR擴(kuò)增亚洲国产福利精品一区在线观看，在接種小麥和傳毒介體中均擴(kuò)增出1.4kb的特異片段，鑒定出小麥藍(lán)矮植原體新寄主7種无码视少妇视频一区二区三区。

amboceptor：介體;雙受體

介蕨屬 Dryoathyrium | 介體;雙受體 amboceptor | 芥;馬芥(綱目)(植典) Brassica cernua Hemsl.; mustard

hinged support：鉸接支點(diǎn), 鉸接支承

lanceolar 矛尖狀的, [植] 披針形的 | hinged support 鉸接支點(diǎn), 鉸接支承 | specific inductive capacity 電容率, 介電常數(shù)

mounting：鑲埋

第二階段是將實(shí)體電路轉(zhuǎn)移到晶圓上,其中包括膜沉積(金屬膜,介電質(zhì)膜..),氧化,擴(kuò)散,離子布植,微影與光蝕刻技術(shù),等制程方式,重復(fù)數(shù)十或數(shù)百次;第三階段是積體電路元件的組裝(Package),包括切割,鑲埋(Mounting),接線,封裝,測試