Reerse transcription was performed with 3 g of total RNA, random hexamer (Amersham Bioscience, Buckinghamshire, United Kingdom), and Superscript II reerse transcriptase at conditions recommended by the manufacture, and then incubated with 1l of RNase at 37oC for 20 minutes.

再根據(jù)生產(chǎn)說明介紹的條件，從中選取3克RNA人人开人人操，隨機六聚物（英國白金漢郡Amersham生物科學(xué)公司生產(chǎn)的）以及逆轉(zhuǎn)錄標記II進行逆轉(zhuǎn)錄国产情侣作爱视频免费观看，用1LRNA酶(Initrogen公司生產(chǎn))在37度的環(huán)境中孵化20分鐘。

About 250 such six oligomers combine to form the virus capsid.

大約250個這樣的六聚物結(jié)合在一起偷偷自拍最新地址，形成了病毒衣殼国产口爆视频。

The effects of different factors on the laser-induced graft polymerization on the PET surface were analyzed, and the mechanisms were discussed. The factors were: 1 Monomers with smaller molecules resulted in better modification than polymeric monomers. Acrylamide monomer was chosen as hydrophilic modifier finally; 2 Higher concentration of the monomer solution favored the graft reaction; 3 With the increasing laser fluence, the surface hydrophilicity of PET films increased at first, then decreased. More laser pulses led to a more hydrophilic surface; 4 Benzophenone as a photosensitizer hindered the graft polymerization; 5 The homopolymer retardant of ammonium iron sulfate hexahydrate in a proper concentration retarded the formation of homopolymers and promotes the graft copolymerization; 6 The solvent of monomer solution also affected the graft reaction. Different solvents functioned in different ways. Ethanol can promote the graft reaction because of the activity of hydrogen in its structure; 7 pH value of the monomer solution affects the polymerization rate, and a lower pH value was favorable to the graft reaction.

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A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本實驗設(shè)計一對兩側(cè)含編碼疏水性氨基酸密碼子的引物，經(jīng)過擴增前導(dǎo)序列10~34aa基因序列91年清纯女神露脸口爆，并重新克隆入質(zhì)粒pRSET-A構(gòu)建串聯(lián)二聚體后最新欧美爆乳日韩精品视频，再利用質(zhì)粒pRSET-A的BamH I / Bgl II同尾酶克隆位點，經(jīng)一系列簡單的酶切和連接日韩黄色免费电影网站，快速構(gòu)建這一前導(dǎo)肽中不含組氨酸標簽序列的串聯(lián)多聚體基因污色视频在线观看，并成功表達其六聚體重組蛋白。

A new functional electroluminescent copolymer is reported in which chromophores and hexa segments alternate along the polymer backbone.

采用Wittig反應(yīng)合成了一種分子主鏈由烷氧基取代的1中文字幕无码专区，4二苯乙烯基苯聚對亞苯基亞乙烯基(PPV的三個單元的齊聚物與柔性隔離鏈段五縮六乙二醇組成的交替型藍綠色發(fā)光共聚物一本在线香蕉高清在线。

A novel method combining a conventional free-radical solution polymerization with a chemical saponification was used to synthesize a, cu-dicarboxyl terminated oligo s with molecular weight below 1500. Here, cn-carboxyl terminated oligo s were firstly synthesized by the free-radical polymerization in THF, where 4,4'-azobis(4-cyanovaleric acid) was used as initiator, thioglycolic acid as chain transfer agent, then CTBMA were transformed into di-CTBMA via saponifying CTBMA in dioxane/H_2O/KOH solution.

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Through observation, research team also found that six CA protein oligomers in the similar amino acid molecules of nitrogen and carbon the end of the connection terminal, which is to understand the stability of HIV virus capsid is of great significance, will help scientists find the interference of such connections method.

通過觀察，研究小組還發(fā)現(xiàn)了六聚物中相近CA蛋白分子的氨基酸氮末端與碳末端的連接方式日韩一级特黄毛片在线看，這對了解HIV病毒衣殼的穩(wěn)定性具有重要意義亚洲av一级二级三级免费观看，有助于科學(xué)家找到干涉這種連接的方法。

The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

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In fact, it consists of four hexamers, each with six monomers.

實際上国产每日，它就是包含了四個六聚物，每個聚合物包含六個單體国产极品一线天视频在线播放。

Scripps Institute of the United States and his馬克耶格爾Professor University of Virginia, the University of Utah colleagues used X-ray crystal photography, the first detailed description of the composition of the HIV virus capsid protein precursors of the hexagonal molecular structure of high-definition, they said hexagonal protein precursors such as CA VI protein oligomers.

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hexamer：六聚物

Hexalithus 六骸面 | hexamer 六聚物 | hexamethyldisilazane 六甲基二硅氮烷

ionomer：離子鍵聚合物

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