Oogonium develops into early oocyte in the ovary, and then the oocyte leaves the ovary for the coelomic fluid in the form of single cell or cell mass followed by the rapid separation of the group of oocytes into individual ones. Oocyte enters into the nephridium after its maturation. The rupture of germinal vesicle marks the oocyte maturation. Oocyte in the coelom does not have follicle membrane and vitelline membrane is formed and developed by the oocyte itself. Smaller oocyte (0μm in diameter) is round, and larger ones (≥60μm in diameter) is ovate. The short and long diameters of a morphologically mature oocyte are about 115—120μm and 140—145μm respectively, and the vitelline membrane is 7—9μm thick.

卵原細(xì)胞在卵巢中發(fā)育至早期卵母細(xì)胞時(shí)期單個(gè)或成團(tuán)脫離卵巢入體腔液中，卵母細(xì)胞團(tuán)細(xì)胞很快分離為單個(gè)細(xì)胞久2无码视频在线观看；卵母細(xì)胞在體腔液中發(fā)育成熟后進(jìn)入腎管；生發(fā)泡破裂是卵母細(xì)胞成熟的標(biāo)志成熟丰满熟妇高潮XXⅩ；體腔中卵母細(xì)胞無(wú)濾泡膜久国产在线，卵黃膜的形成與發(fā)育靠卵母細(xì)胞本身；卵徑小于60μm的卵母細(xì)胞呈圓形国产在线A级在线视频，卵徑大于60μm 的卵母細(xì)胞為卵圓形jiZZjiZZjiZZjiZZ免费看，形態(tài)上成熟的卵母細(xì)胞短徑約115—120μm、長(zhǎng)徑約140—145μm9999视频免费视频、卵黃膜厚7—9μm美女裸体污污18禁免费APP。

The oogenesis of P. esculenta can be divided into the following phases in morphological characteristics of oogonium and oocyte development: the proliferative phase of oogonium (0μm in diameter), the initial growth phase of oocyte (10—20μm in diameter), the grand growth phase-Ⅰof oocyte (20—60μm in diameter), the grand growth phase-II of oocyte (60μm×70μm to 120μm×145μm in size), the mature phase of oocyte, and the declining phase, showing a dynamic changes in oogenesis of P. esculenta .

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Hormone level in maturation medium affected cumulus expansion of COC, but cumulus expansion became less dependent on hormone level with time of culture. Porcine immatured oocytes, granulosa cells from small antral follicles and cumulus cells of immatured oocytes secreted cumulus-expansion enabling factors. Granulosa cells from 3-6mm follicles did not produce CEEF, but there was some CEEF in the follicular fluid of this sized follicles.

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Hormone level in maturation medium affected cumulus expansion of COC,but cumulusexpansion became less dependent on hormone level with time of culture.Porcine immaturedoocytes,granulosa cells from small antral follicles and cumulus cells of immatured oocytessecreted cumulus-expansion enabling factors.Granulosa cells from 3-6mm follicles did notproduce CEEF,but there was some CEEF in the follicular fluid of this sized follicles.Sun xingshen, Animal Histology and Embryology

激素水平對(duì)卵丘擴(kuò)展有影響日日摸日日碰夜夜爽97无码，但隨培養(yǎng)時(shí)間的延長(zhǎng)天天色天天吹天天操天天日天天爽，對(duì)激素的要求有逐步減少的趨勢(shì)；豬卵丘擴(kuò)展不依賴(lài)于卵母細(xì)胞免费看三级网站，卵母細(xì)胞核成熟也與卵丘擴(kuò)展無(wú)關(guān)国产午夜无码福利在线看网站；GV期卵母細(xì)胞、小腔卵泡內(nèi)顆粒細(xì)胞和成熟卵的卵丘細(xì)胞都有較高的分泌CEEF能力免费看黄的视频；3-6mm卵泡的壁顆粒細(xì)胞不能分泌CEEF亚洲人v永久无码天堂网，但3-6mm卵泡的卵泡液中有CEEF。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分為4個(gè)部分免费成年人网址，第一部分對(duì)影響豬卵母細(xì)胞體外成熟和孤雌激活后胚胎分裂的幾個(gè)重要條件進(jìn)行了比較研究午夜黄色精品视频在线播放，確立了一種較好的培養(yǎng)方法：與顆粒細(xì)胞共培養(yǎng)国产尤物电影在线观看网站免费，找到了一種適合豬卵母細(xì)胞體外成熟的培養(yǎng)基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分別為(86.7±3.35)%和(86.3±4.16)%韩国午夜理论片在线观看手机，國(guó)外報(bào)道的最高成熟率為(85.7±4.1)%拍自拍亚洲手机黄色视频在线观看；第二部分對(duì)豬卵母細(xì)胞孤雌激活的化學(xué)方法進(jìn)行了研究，確立了化學(xué)激活豬卵母細(xì)胞的兩種最佳方法：1將成熟的去卵丘顆粒細(xì)胞的MII期卵母細(xì)胞用20μmol/Lionomycin作用30min欧美两性一区，再將卵母細(xì)胞培養(yǎng)于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培養(yǎng)液中日韩欧美国产一，卵裂率和桑囊胚發(fā)育率達(dá)到(76.7±7.6)%和(37.1±6.4)%2將成熟的去卵丘顆粒細(xì)胞的MII期卵母細(xì)胞在200μM/L的Thimerosal中處理20min，再與8mM的DTT共孵育30min黄色视频免费关观看网站，卵裂率和桑/囊胚形成率為(81.0±2.8)%和(39.6±2.7)%在线免费av网址；第三部分對(duì)孤雌激活胚胎的培養(yǎng)條件進(jìn)行了研究，確立了一種最佳的胚胎培養(yǎng)條件：在SOF簡(jiǎn)單培養(yǎng)基中添加顆粒細(xì)胞進(jìn)行前3天的培養(yǎng)国产永久免费BBw，然后轉(zhuǎn)入添加胎牛血清的NCSU—23培養(yǎng)基并和輸卵管上皮細(xì)胞進(jìn)行后期的培養(yǎng)欧美性爱视频一级黄片，其桑椹胚和囊胚的發(fā)育率為(59.5±3.2)%；第四部分研究了IGF-I

Following chemical activation, blastocysts rate of the treated oocytes was similar to untreated oocytes.8 Following fertilization, however, few oocytes inhibited with CHX developed into morulae/blastocysts, due to a high incidence of polyspermy.9 Cortical granule migration occurred during inhibition, but CHX inhibition impaired CG migration, significantly no oocytes inhibited by CHX completed CG migration after maturation.10 CHX inhibition had no effects onα-microtubles and microfilaments of goat oocytes.

抑制24h轉(zhuǎn)為正常培養(yǎng)24h处破女免费观看，不影響卵母細(xì)胞的成熟和孤雌激活能力深夜免费在线观看网站，并且CHX抑制后再成熟的卵母細(xì)胞經(jīng)孤雌激活發(fā)育到囊胚的比例與對(duì)照組卵母細(xì)胞相似。8国产yyzz、體外受精后啪啪一级视频，CHX抑制后再成熟卵母細(xì)胞的多精受精現(xiàn)象顯著增加，發(fā)育到桑椹胚/囊胚的比例顯著低于對(duì)照組特黄a大片免费视频。9日韩欧美人妻综合在线、CHX抑制過(guò)程中皮質(zhì)顆粒仍能發(fā)生遷移，但是CHX抑制會(huì)對(duì)皮質(zhì)顆粒的遷移造成不可恢復(fù)的損傷国产zaixian自拍，使再成熟的卵母細(xì)胞內(nèi)皮質(zhì)顆粒不能完全遷移国产超碰人人做人人爱。10、CHX抑制對(duì)山羊卵母細(xì)胞α-微管和微絲都沒(méi)有影響国产91Av九色，無(wú)論是抑制后處于生發(fā)泡期的卵母細(xì)胞18岁不能看的网址，還是抑制后再成熟的卵母細(xì)胞，微管和微絲的分布都與對(duì)照卵母細(xì)胞相似1024看片无码人妻日韩。

objective we start with clinical research to explore the ability and mechanism of ion conduction with chinese herbs to curing ovulatory obstacle.methods adopting the methods of clinical observation random contrast.select the cases of ovulatory obstacle dividing randomly into two groups:therapeutic group and control group,the patients of therapeutic group using ion conduction with chinese herbs with act'on nourishing blood and promoting blood circulation to dissipate blood stasis when follicle grow up to 1.4cm.we take the decoction of jia jian tao hong si wu tang conducting the unilateral lower abdomen which has the dominant follicle,treating continuously for 4～8 days and observing ovulation.the patients of control group inject hcg 10000unit when follicle grow up to 1.8cm and observing ovulation.appraising two groups ovulated rate and pregnancy rate,and detect fsh国产A片123区、lh、e2后λ式吃奶动态后λ式视频、p at sixteen to eighteen days of menstrual cycle.results ion conduction with chinese herbs can significantly inspires ovulation and its effection is evidently higher than control group and has a higher pregnancy rate,no side effect of ovarian hyperstimulation syndrome.conclusion ion conduction with chinese herbs has significant function on inspiring ovulation.the target effection of treating ovulatory obstacle is exact.this method has a ovulated rate and higher pregnancy rate.

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In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我們?cè)谌〉脙煞N不同品系小鼠的卵丘卵母細(xì)胞復(fù)合體后，先將卵丘卵母細(xì)胞復(fù)合體置于含有多種生長(zhǎng)因子和激素的MEM培養(yǎng)液中培養(yǎng)6小時(shí)98神马午夜，此時(shí)卵母細(xì)胞已進(jìn)入第一次減數(shù)分裂的前中期日韩成人丝袜在线视频，并且在DIC倒置顯微鏡下可以看到濃縮的染色體，用Polscope可以發(fā)現(xiàn)明顯的紡錘體老嫖客精品视频在线播放，借助這種顯微鏡通過(guò)顯微操作將兩種不同品系小鼠來(lái)源的卵母細(xì)胞的MI紡錘體進(jìn)行互換可以免费看黄的网址，經(jīng)過(guò)三次直流電脈沖作用后，分別有91.6％的胞質(zhì)—MI核質(zhì)體對(duì)融合永久黄网播放站色视频免费，經(jīng)過(guò)進(jìn)一步的培養(yǎng)后欧美激情免费性爱，超過(guò)80％的重組卵母細(xì)胞排出第一極體，體外受精后分別有79％和77％的重組卵形成雙原核seqiqi999，受精后的胚胎在KSOM胚胎培養(yǎng)液中體外培養(yǎng)4天后色色色色香蕉，超過(guò)60％的胚胎發(fā)育至囊胚。

The results show that: 1 supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free control, but cumulus expansion is not necessarily related to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2 exposure of pig COCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturation compared to that for 46-48 hours; 3 under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4 the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5 different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.

結(jié)果顯示：(1)在成熟液中添加蛋白質(zhì)可以加強(qiáng)卵丘細(xì)胞的擴(kuò)散午夜AV，但豬卵母細(xì)胞的核成熟與其周?chē)?strong>卵丘細(xì)胞擴(kuò)展沒(méi)有必然的聯(lián)系日韩乱子伦免费视频，卵丘細(xì)胞擴(kuò)散或成放射狀不宜作為豬卵母細(xì)胞核成熟的標(biāo)準(zhǔn)，只有排出第一極體才能作為豬卵母細(xì)胞核成熟的標(biāo)志一级免费ww视频；(2)在豬COCs的46-48小時(shí)成熟培養(yǎng)的后23-24小時(shí)階段去除成熟液中的激素不但可以保證卵母細(xì)胞的核成熟率欧美色污在线视频，而且可加強(qiáng)卵丘細(xì)胞的擴(kuò)散；(3)在現(xiàn)有實(shí)驗(yàn)條件下亚洲色婷婷五月综合在线观看，在mTCM和mNCSU中添加10％pFF與在mNCSU中添加10％FCS相比可獲得較高的豬卵母細(xì)胞核成熟率欧洲日韩一区二区免费视频网站；(4)在不同的成熟液中添加不同的蛋白質(zhì)對(duì)豬卵母細(xì)胞核成熟率的影響效果不一樣欧美精品免费A片在线12页，但對(duì)體外受精后的早期胚胎發(fā)育影響不明顯；(5)成熟液種類(lèi)對(duì)豬卵母細(xì)胞的卵丘細(xì)胞擴(kuò)散和體外受精后的早期胚胎發(fā)育無(wú)顯著影響老年黄色视频免费网站。

The cumulus cells of OCC were cut off and dispersed by 1 mL syringe. The cumulus cells were co-cultured with the immature oocytes retrieved from the COH cycles after they adherent to the bottom of the dish. The immature oocytes were experienced IVM procedures in different culture media. They were divided into 3 groups(the oocytes at germinal vesicle stage from one woman were allotted to the same group randomly). Group 1: basic culture medium+ human follicular fluid; Group 2: solution A+ cumulus cells; Group 3: solution A+ OCC+ follicle stimulating hormone + epidermal growth factor. Then, the maturation rate, fertilization rate and formation rate of available embryo were observed.

在控制性促排卵周期有未成熟卵母細(xì)胞時(shí)4455vs永久亚洲，將同周期成熟卵丘復(fù)合體切出部分卵丘細(xì)胞，用1 mL注射器抽打分散細(xì)胞毛片高清无码在线，貼壁培養(yǎng)国产二级三级精品。113個(gè)治療周期中，298枚生發(fā)泡期卵母細(xì)胞經(jīng)3種不同培養(yǎng)液體外成熟培養(yǎng)(同一病人的生發(fā)泡期卵被隨機(jī)分到某同一組中)：第1組28個(gè)周期中73枚：基礎(chǔ)培養(yǎng)液+卵泡液欧美久草黄色网站三级片；第2組40個(gè)周期中115枚：A液+分散貼壁的卵丘細(xì)胞国产wwwjizz；第3組45個(gè)周期中110枚：A液+分散貼壁的卵丘細(xì)胞+促卵泡生成激素+表皮生長(zhǎng)因子。

blastomere：(卵)裂球

卵裂產(chǎn)生的細(xì)胞稱(chēng)為囊胚細(xì)胞或卵裂球(blastomere). 卵裂球只是數(shù)量增多,每個(gè)卵裂球沒(méi)有生長(zhǎng),卵裂球緊挨在一起,總體沒(méi)有增大. 第一次卵裂和第二次卵裂都是縱向分裂,但兩者的分裂面相互呈直角,把受精卵垂直分裂成4個(gè)體積相同的卵裂球.

chorion：卵殼

使卵有一個(gè)適宜的生長(zhǎng)發(fā)育環(huán)境.產(chǎn)卵于植物組織.三,卵的基本結(jié)構(gòu) 昆蟲(chóng)的卵是一個(gè)大型細(xì)胞.最外面是起保護(hù)作用的卵殼(chorion),卵殼里面 的簿層稱(chēng)卵黃膜(vitelline membrane)圍著原生質(zhì),卵黃及核.豐富的卵黃 充塞在原生質(zhì)網(wǎng)絡(luò)的空隙內(nèi),

egg envelope; e. membrane：卵封;卵外膜

egg cell 卵細(xì)胞 | egg envelope; e. membrane 卵封;卵外膜 | egg jelly 卵膠

egg membrane; e. envelope：卵封;卵外膜

egg mass 卵塊 | egg membrane; e. envelope 卵封;卵外膜 | egg pedicle 卵梗

fraternal twins：異卵雙生

因而,同卵雙生和異卵雙生( fraternal twins)之間各種傾向的不同是由基因上的差別決定的. 斯佩克特指出,這項(xiàng)研究結(jié)果顯示,同卵雙生的性高潮的經(jīng)歷比異卵雙生的經(jīng)歷更加相似. 而同卵雙生與異卵雙生之間的差別主要存在于基因上,

non-identical twins：非同卵雙生 ,非同卵孿生,非同卵孿生兒

non-flowering plant 無(wú)花植物 | non-identical twins 非同卵雙生 ,非同卵孿生,非同卵孿生兒 | non-living thing 非生物

oogonium：卵原細(xì)胞

卵子的發(fā)生過(guò)程包括卵原細(xì)胞(Oogonium)的增殖视频日韩最新无码迅雷下载、卵母細(xì)胞(Oocyte)的生長(zhǎng)和卵母細(xì)胞的成熟三個(gè)階段. 在胚胎期性別分化后,雌性胎兒的原始生殖細(xì)胞便分化為卵原細(xì)胞. 卵原細(xì)胞與其它細(xì)胞一樣含有典型的細(xì)胞成分;

oospore：卵孢子

(1)卵孢子(oospore):卵菌的有性孢子. 是由兩個(gè)異型配子囊--雄器和藏卵器接觸后,雄器的細(xì)胞質(zhì)和細(xì)胞核經(jīng)授精管進(jìn)入藏卵器,與卵球核配,最后受精的卵球發(fā)育成厚壁的日韩男女你懂的在线观看、雙倍體的卵孢子. (2)接合孢子(zygospore):接合菌的有性孢子.

ovulation：排卵;放卵

卵胎生 Ovoviviparity | 排卵,放卵 Ovulation | 卵,小卵 Ovule,Ovum,Egg

vitelline：卵黃(的);蛋黃(色)的;類(lèi)似卵黃的

vitellin 卵黃磷蛋白 | vitelline 卵黃(的);蛋黃(色)的;類(lèi)似卵黃的 | vitellolutein 卵黃黃質(zhì);卵黃黃素