One of two sperm get into an archegonium and fused with egg. The nucleus of fertilized egg began to divide, and the walls were formed after the third division. The eggs without fertilization divided two times and then disappeared.

雄配子體產(chǎn)生的兩個(gè)精子中的一個(gè)進(jìn)入頸卵器與卵細(xì)胞結(jié)合形成受精卵怡红院宜春院十次综合啦首页，受精卵核到第3次分裂形成細(xì)胞壁，沒有受精的卵細(xì)胞還要進(jìn)行兩次有絲分裂后才消失亚洲中日韩欧美精品一区二区。

And the research materials were often meiotic pachytene chromosomes of sex gland and mitotic prometaphase of early embryos and diapause eggs\' somatic cells.

早期黄色网站视频网址，家蠶染色體識(shí)別和基因定位研究主要利用性腺減數(shù)分裂粗線期的染色體、早期胚胎和滯育卵有絲分裂早中期的染色體作為材料中国一级A片XXXX，按照相對(duì)長(zhǎng)度和部分限性易位系統(tǒng)或缺失等來進(jìn)行核型分析日韩人人操人人摸。

It takes about 0.5 h for a pollen tube from germination to arrive at the micropyle. About 0.5 h after pollination, the pollen tube releases two sperms into one synergid. At 0.5-2.5 h after pollination, the egg cell fuses with one sperm to form a zygote. About 10.0 h after pollination, the division of the zygote begins. The duration of the zygote lasts about 2.5-10.0 h. However, one sperm nucleus fuses with two polar nuclei at 1.0-3.0 h after pollination, and 5.0 h after pollination the primary endosperm nucleus begins to divide.

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The cleavation rates and blastocyst development rates of cloned embryos were used to assess the efficiency of different operational procedure. Finally, the best combination of operational procedure, that the spindle-viewer system was used for oocytes enucleating, and donor cell was electrofused into ooplasm by electrical pulse (1.9 kV/cm, 10 ms, two) to reconstruct bovine cloned embryos.

以核移植胚胎的卵裂率女被男啪到爽的视频网站、囊胚發(fā)育率作為檢測(cè)指標(biāo)，對(duì)不同的方法所獲得的克隆胚胎的卵分裂率與囊胚發(fā)育率進(jìn)行比較五月婷婷精品视频6，最后篩選獲得1個(gè)優(yōu)化的牛體細(xì)胞核移植操作程序国产污片免费，即采用Spindle view系統(tǒng)對(duì)牛卵母細(xì)胞進(jìn)行去核操作，將供核體細(xì)胞注射到卵周隙一级黄色视频大片，然后通過電融合法將供體核引入去核卵細(xì)胞質(zhì)(電融合參數(shù)為1.9 kV/cm强插在线观看，脈沖時(shí)程10 ms，方波2次間隔2 s)女的被男的c的免费视频。

In conclusion, these results suggested that (1) the ageing-associated decline in fertility of female KM mouse was due to a decrease in both quantity and quality of oocytes;(2) the ooplasm from young mice did not correct the meiotic errors of aged mice, and the ooplasm from aged mice did not induce abnormal segregation of meiotic chromosome of young mice, indicating that meiotic anomalies found in the oocytes of aged mice might be related to nucleus or chromosomes and relevant factors;(3) a critical nucleocytoplasmic ratio was essential for normal maturation and segregation of meiotic chromosomes of oocytes and development to 2-cell embryos.

本研究結(jié)果表明：（1）昆明白小鼠與衰老相關(guān)的生育力下降是其卵母細(xì)胞數(shù)量減少和質(zhì)量下降的綜合結(jié)果搞那个黄色片来看一下；（2）年輕小鼠卵母細(xì)胞細(xì)胞質(zhì)不能糾正老齡小鼠GV的減數(shù)分裂錯(cuò)誤，老齡小鼠卵母細(xì)胞細(xì)胞質(zhì)也不誘導(dǎo)年輕小鼠的GV發(fā)生減數(shù)分裂錯(cuò)誤国内精品三级三级精品二区，老齡小鼠卵母細(xì)胞減數(shù)分裂異常很可能與細(xì)胞核或染色體及其相關(guān)因素有關(guān)高清无码DVD；（3）關(guān)鍵的核質(zhì)比對(duì)卵母細(xì)胞正常的成熟欧美性爱日韩、減數(shù)分裂染色體分離及2-細(xì)胞期胚的發(fā)育是絕對(duì)必需的最爽高潮午夜视频。

The results showed that the rates of mature oocyte were 75.2％, 73.1％, 69.8％, 63.5％and oocyte at telophase of MI were 16.3％, 15.9％, 16.9％, 27.0％, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P＜0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P＜0.05); Significant higher rates (66.6％, 66.6％, 73.6％) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3％)(P＜0.05). The rates of oocyte with cortical granules in cortex were 58.8％, 33.9％, 54.7％and 47.9％respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P＜0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.

三、EGF和TGFα對(duì)卵母細(xì)胞成熟的影響未成熟卵母細(xì)胞分別在TCM199基礎(chǔ)培養(yǎng)液+血清99久久精品国产免费男女、TCM199基礎(chǔ)培養(yǎng)液+BSA+EGF欧美老人牲交免费一级A片、TCM199基礎(chǔ)培養(yǎng)液+BSA+TGFα和TCM199基礎(chǔ)培養(yǎng)液+BSA四組成熟培養(yǎng)系統(tǒng)中成熟培養(yǎng)，22小時(shí)后上述各組卵母細(xì)胞的核成熟率分別為75.2％欧美黄色午夜、73.1％欧美三級片AAAA、69.8％、63.5％日日夜人人操，處于第一次減數(shù)分裂末期的比率分別為16.3％东北粗口国产床、15.9％、16.9％工口视频亚洲、27.0％亚洲一级Av无码毛片无卡，在添加血清、EGF在线播放欧美特级黄色视频、TGFα各組中核成熟率顯著高于其它各組(P＜0.05)www.huicheng99.com，處于末期的比例明顯低于其它各組(P＜0.05)：添加血清、EGF一区一本久久、TGFα各組在a-Tubulin蛋白的分布與表達(dá)上(66.6％天天插天天狠天天透伊人、66.6％、73.6％)明顯高于BSA組的正常率43.3％(P＜0.05)唯美囗交视频系列全集；各組CG發(fā)生遷移較好的卵母細(xì)胞的比率分別為58.8％AV无码永久免费网站、33.9％、54.7％丝瓜视频成人无限看免费下载、47.9％国内主播直播在线观看，血清組和添加EGF組CG遷移至皮質(zhì)區(qū)的比例明顯高于僅添加BSA處理組(P＜0.05)自拍高潮日韩在线视频。

Most of the a-sexual embryo sacs also parthenogenetically developed into asexual seeds,which is the same as that of their maternal parent.

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The fertilization was processed by sperm in mature male gametophyte combining with egg cell on the top of archegonia on June 15 or so.

摘要樟子松發(fā)育成熟的雄配子體中的精子6月15日左右在頸卵器中上部與卵細(xì)胞結(jié)合幸爱五月天，進(jìn)行受精作用，其后自偷自拍亚洲一区二区，受精卵進(jìn)行游離核分裂欧美性爱亚洲色图，形成8個(gè)子核時(shí)，開始形成細(xì)胞壁97国产乱伦无码。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我們?cè)谌〉脙煞N不同品系小鼠的卵丘卵母細(xì)胞復(fù)合體后www.zipai，先將卵丘卵母細(xì)胞復(fù)合體置于含有多種生長(zhǎng)因子和激素的MEM培養(yǎng)液中培養(yǎng)6小時(shí)，此時(shí)卵母細(xì)胞已進(jìn)入第一次減數(shù)分裂的前中期最近中文字幕免费大全下载，并且在DIC倒置顯微鏡下可以看到濃縮的染色體无码3p，用Polscope可以發(fā)現(xiàn)明顯的紡錘體，借助這種顯微鏡通過顯微操作將兩種不同品系小鼠來源的卵母細(xì)胞的MI紡錘體進(jìn)行互換91免费视频网，經(jīng)過三次直流電脈沖作用后亚洲色黄hAV在线，分別有91.6％的胞質(zhì)—MI核質(zhì)體對(duì)融合，經(jīng)過進(jìn)一步的培養(yǎng)后老太婆黄色片，超過80％的重組卵母細(xì)胞排出第一極體日本中文字幕系列网址，體外受精后分別有79％和77％的重組卵形成雙原核，受精后的胚胎在KSOM胚胎培養(yǎng)液中體外培養(yǎng)4天后一级黄色网站片AV，超過60％的胚胎發(fā)育至囊胚国产激情A∨在线视频播放。

Most sperm nuclei eventually developed into the male pronuclei, just like the normal sperms, except sperm nuclei in a few zygotes kept dense throughout the fertilization process. Dense chromosome body was seen at the nuclear area during the prophase of first mitosis, and in the middle of spindle at metaphase of first mitosis.

精子入卵后形成精核，大多能夠逐漸解凝乱伦视频综合网、液化并膨大色狠狠影视一区二区三区，最終形成形態(tài)正常的雄性原核，但在第一次卵裂早期退化成濃縮的染色質(zhì)小體欧美一级婬片人妻大片，并在胞質(zhì)分裂時(shí)隨機(jī)地分配到其中一個(gè)子細(xì)胞里的分裂溝附近国产福利在线影院。

blastoderm：胚盤

現(xiàn)在來看果蠅(Drosophila)的例子:在果蠅的早期胚胎發(fā)育中,最初一些有絲分裂并 不伴有細(xì)胞分割.經(jīng)過幾次核分裂后,細(xì)胞核移到胚的表面,形成胚盤(blastoderm).其 中少數(shù)幾個(gè)核位于卵后端的極細(xì)胞質(zhì)(pole plasm)中,在這些核的周圍立即形成細(xì)胞膜,

oogonium：藏卵器

進(jìn)行有性生殖時(shí),部分菌絲細(xì)胞分化為雄器(antheridium)和藏卵器(oogonium)兩種配子囊,二倍體細(xì)胞核在發(fā)育的雄器和藏卵器中發(fā)生減數(shù)分裂,產(chǎn)生單倍體細(xì)胞核;雄器和藏卵器先是以配子囊接觸交配的方式進(jìn)行質(zhì)配,兩個(gè)單倍體細(xì)胞核在藏卵器中進(jìn)行核配,

Oogonium,Ovogonium：原卵細(xì)胞

卵子發(fā)生 Oogenesis | 原卵細(xì)胞 Oogonium,Ovogonium | 卵核分裂 Ookinesis

reduction division：減數(shù)分裂

減數(shù)分裂(Reduction division) Van Beneden(1883)發(fā)現(xiàn)當(dāng)蛔蟲的卵在受精時(shí)雄蟲精子的兩個(gè)染色體和卵子細(xì)胞 核的兩個(gè)染色體聯(lián)合,形成具有四個(gè)染色體的合子的新細(xì)胞核.

meroblastic egg：不全裂卵;偏裂卵

局部分裂的;偏裂卵 meroblastic | 不全裂卵;偏裂卵 meroblastic egg | 剩余精核 merocyte

ookinesis：卵核分裂

卵原細(xì)胞 oogonium | 卵核分裂 ookinesis | 透螟 oolemma

ookinete：合子運(yùn)動(dòng)期;動(dòng)合子

卵核分裂 ookinesis | 合子運(yùn)動(dòng)期;動(dòng)合子 ookinete | 卵黃膜;明帶 oolemma

ookinete：合子運(yùn)動(dòng)期

卵核分裂 Ookinesis | 合子運(yùn)動(dòng)期 Ookinete | 卵黃膜,昆蟲 Oolemma

oolemma：透螟

卵核分裂 ookinesis | 透螟 oolemma | 卵巢切除術(shù) oophorectomy

segmentation sphere：卵割球

卵割核 segmentation nucleus | 卵割球 segmentation sphere | 卵割;分裂;環(huán)節(jié)形成 segmentation; cleavage