Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭質粒YCEp1為載體構建西方許旺酵母部分基因組文庫高清无码黄三级，在大腸桿菌中擴增后提取混合質粒DNA欧美亚性爱大片，經電轉化非缺陷標志酒精酵母 AS.2.1364，在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力篩選含水解淀粉的陽性轉化子欧美精品免费观看，從陽性轉化子中分離重組質粒證實含5.0kb的插入片段，用α-淀粉酶基因兩端序列設計的引物PCR擴增及擴增片段序列分析證實該片段中含有α-淀粉酶全部編碼序列黄网免费直接观看。

Our data revealed glucose and beef extract to be the most influential parameters, and the composition of the optimal fermentation medium (100 mL) was determined to consist of 4.46 g glucose, 2 g soluble starch, 4 g silkworm powder, 2.93 g beef extract, 0.10 g KH2PO4 and 0.15 g MgSO4·7H2O.

最后通過中心組合設計和響應面分析法十八禁免费A片AV毛片无码网站，確定了主要影響因子的濃度。優(yōu)化后的培養(yǎng)基組成為：葡萄糖4.46 g/100 mL18禁成年无码免费网站无遮，可溶性淀粉2 g/100 mL精品国自产在线观看，蠶蛹粉4 g/100 mL，牛肉膏2.93 g/100 mL岛国一级高潮av黄片，KH2PO4 0.1 g/100 mL和MgSO4·7H2O 0.15 g/100 mL欧美午夜免费福利。

The purified XynIII could hydrolyze birchwood xylan, oat spelt xylan, lichenin, and barley β-glucan, but not CMC, avicael cellulose, or soluble starch under the assay conditions in this study.

底物特異性鑒定，XynⅢ能夠水解樺木木聚糖欧美久久迷奸网站视频最新、燕麥木聚糖国产日韩Av无码毛片尤物、地衣多糖和大麥β-葡聚糖，對羧甲基纖維素午夜国产三级、微晶纖維素以及可溶性淀粉則沒有活性看黄名站在线，是一種雙功能酶。

The production medium was optimized both by "one-variable-at-a-time" approach and the statistical methods based on Response Surface Method. The optimized medium composition was as follows: soluble starch 65.45 g/L, glucose 9.30 g/L, soybean flour 10 g/L, peptone 5 g/L, fish flour 10 g/L, yeast extract 3 g/L,(NH_4)SO_4 1.5 g/L, MgSO_4·7H_2O 4.93 g/L, KH_2PO_4 0.2 g/L, NaNO_3 0.75 g/L, CaCO_

通過單因素試驗和以響應面為主的統(tǒng)計學方法對發(fā)酵培養(yǎng)基進行了優(yōu)化国产乱伦视频网址，優(yōu)化后的發(fā)酵培養(yǎng)基組成為：可溶性淀粉65.45g／L一区二区福利导航在线，葡萄糖9.30g／L，豆餅粉10g／L五月婷网址，蛋白胨5g／L91网站免费看国产，魚粉10 g／L，酵母粉3 g／L在线播放免费人成试看，(NH_4)_2SO_4 1.5g／L东北乱伦视频，MgSO_4·7H_2O 4.93g／L，KH_2PO_4 0.2g／L快播一级毛片，NaNO_30.75g／L天堂欧美成公司，CaCO_34g／L。

As carbon sources, soluble starch and maltose are better; saccharose and glucose are good; glycerin and mellifluence are not suitable.

可溶性淀粉和麥芽糖為較好碳源96免费高清的视，其次是蔗糖和葡萄糖久久久久夜夜夜一级A片水蜜桃，甘油和甘露醇是黑脈羊肚菌菌絲不能很好利用的碳源。

The optimum carbon source for mycelial growth was soluble starch,and the peptone is the optimum nitrogen source.

菌絲生長最適碳源是可溶性淀粉三级片AAAA，最適氮源是蛋白胨18在线观看黄网站。

The results showed that all contents were lower in male sterilie anthers than in fertilisable ones.With anther developing,the contents of soluble starch,protein and free proline increased obviously in male fertilizable anthers,but not in sterile ones.

結果表明：太空誘變處理玉米核不育材料的可育株花藥中的可溶性糖美国一级婬片A免费看、淀粉、蛋白質以及游離脯氨酸含量均高于不育株花藥的人人操人人色人人爽，且可育株花藥中的可溶性淀粉亚洲欧美另类在线网址、蛋白質和脯氨酸含量都隨著花藥的發(fā)育逐漸累積，在不育株花藥中這些指標的變化不明顯亚洲国产黄色视频。

The contents of soluble sugar , starch , protein and free praline in anthers were analyzed and compared between male fertilizable and male sterilie induced by space flight at different developmental stages .

為探討太空誘變處理玉米核不育材料的可育株欧美A区福利在线，不育株花藥的生理生化特性差異，對可育性交AAAAA片在线看，不育材料不同發(fā)育時期花藥的可溶性糖色伊人国产在线等李杰，可溶性淀粉，蛋白質含量以及游離脯氨酸含量進行了測定欧美日韩福利视频一区。

The results in shake flask indicated that the maximam yield was obtained at 60h, the transformation rate of carbon source was 43.5% with 2% inocula; and the normal involved volume had no obvious effect on fermentation.

搖瓶結果表明：以可溶性淀粉作發(fā)酵培養(yǎng)基的主要碳源性爱一级，2%接種量，發(fā)酵60h產酸濃度最高欧美久久黄色网站，發(fā)酵液中富馬酸對淀粉的轉化率為43.5%美国性生活黄片视频，在適量的情況下，裝液量對發(fā)酵幾乎沒有影響欧美老妇大乳大屁股操逼视频。

In this paper, cenosphere , fly ash , coal gangue, and diatomite are used as raw materials of aggregate; bentonite, glass and sodium silicate as binder materials; charcoal powder, coal powder, common starch and soluble starch as pore forming m.

本文以漂珠欧美性XXⅩXX、粉煤灰、煤矸石和硅藻土為骨料原料欧美44444色，膨潤土91欧美性爱视频、玻璃粉和水玻璃為粘結劑原料，炭粉黄色视频大色、煤粉欧美很黄C视频、食用淀粉和可溶性淀粉為造孔劑原料，采用模壓成型和常規(guī)燒結法制備了礦物多孔材料www、9557pm、com。

amylodextrin：淀粉糊精;可溶性淀粉

澱粉酵素 amylase | 澱粉糊精,可溶性澱粉 amylodextrin | 澱粉球朊,膠化纖維素 amyloid

amylogen：可溶性淀粉

"淀粉糊精","amylodextrin" | "可溶性淀粉","amylogen" | "淀粉葡萄糖苷酶","amyloglucosidase"

diastase：淀粉糖化酶

1833年佩延(Payen)和Persoz從麥芽中抽提出一種對熱敏感的物質,這種物質能將淀粉水解成可溶性糖,被稱為淀粉糖化酶(diastase),意思是"分離". 所以后人命名酶時常加詞尾-ase. 由于他們用乙醇沉淀等方法提純得到了無細胞的酶制劑,

diastase：糖化酶

1833年佩延(Payen)和Persoz從麥芽中抽提出一種對熱敏感的物質,這種物質能將淀粉水解成可溶性糖,被稱為淀粉糖化酶(diastase),意思是"分離". 所以后人命名酶時常加詞尾-ase. 由于他們用乙醇沉淀等方法提純得到了無細胞的酶制劑,

Clitocybe maxima：大杯傘

大杯傘(Clitocybe maxima)華農2號菌株具有分泌漆酶的能力,與靜止培養(yǎng)相比,以110r/min搖床培養(yǎng)能明顯促進大杯傘華農2號菌株漆酶的分泌. 以25g/L可溶性淀粉為碳源,2.0g/L酪蛋白胨為氮源,銅離子添加量為0.1mmol/L進行液體培養(yǎng),

soluble saccharide：可溶性糖類

soluble rna 可溶性核糖核酸 | soluble saccharide 可溶性糖類 | soluble starch 可溶性淀粉

Ura：尿嘧啶

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amylolytic enzyme：淀粉酶

amylodextrine 可溶性淀粉 | amylolytic enzyme 淀粉酶 | amylometer 淀粉計

soluble stanching gauze：可溶性止血紗布

soluble powder 可溶性粉劑 | soluble stanching gauze 可溶性止血紗布 | soluble starch 可溶性淀粉

Soluble carbohydrates：可溶性糖

可溶性淀粉:soluble-starch | 可溶性糖:Soluble carbohydrates | 可溶性糖:soluble sugars