OBJECTIVE: To construct a prokaryotic expression plasmid of the main catalytic domain of dipeptidyl peptidase of murine fibroblast activation protein αand obtain the target fusion protein in order to provide basis for production of antibodies and anti-tumor vaccine targeting FAPα.

目的：構(gòu)建小鼠成纖維細(xì)胞激活蛋白α二肽基肽酶催化核心序列的高效原核表達(dá)載體，進(jìn)行該序列的原核表達(dá)人人艹人人黄色、純化和復(fù)性鲁鲁鲁www，為下一步高效抗體制作和以FAPα為靶標(biāo)的抗腫瘤疫苗研發(fā)提供實(shí)驗基礎(chǔ)。

Vitamin E, or tocopherol, is a lipid-soluble antioxidant and essential for human health. 2-methyl-6-phytyl-1, 4-benzo- quinol methyltransferase, one of the key enzymes in vitamin biosynthetic pathway in plants, converts 2-methyl- 6-phytyl-1,4-benzoquinol to 2,3-dimethyl-6-phytyl-1,4-benzoquinol.

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Objective: To verify whether hot PBS washing after the enzymatic reaction between tyramine and horseradish peroxidase can improve the sensitivity and specificity of catalyzed signal amplification. Methods: Using 7 different types of primary antibodies and 2 tomor microarray system, we carried out EnVision, LSAB, Standard CSA, and modified CSA staining sod compared their sensitivities and specificities. Results: The modified CSA method was the moat sensitive one among the 4 methods.

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objective: to achieve the soluble expression of mn-sod gene in e.coli and assay the enzyme activity of the expressed product. methods: the coding region of superoxide dismutase was amplified using pcr method from the e.coli genome. the pcr product was cloned into puc19-t vector and sequenced.in addition,the cloned coding region of mn-sod was inserted into the expression vector pet-28a to form the recombinant plasmid pet-28a-mn-sod and was then transformed into e.coli bl21 for expression.

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Bilirubinic decomposition product has more powerful antioxidation than vitamin E;dissociative ferrum also plays roles on harmful product such as hydroxy radical and superoxide anion etc;As an important signal molecules,CO play significant roles on neuron information transfer and vascular tone regulation;people pay attention to HO,which is the main enzymatic system producting endogenous CO increasingly.

其中膽綠素的分解產(chǎn)物具有較維生素E更強(qiáng)的抗氧化作用；Fe~(2+)參與催化生成有害的活性氧代謝物麻豆精品无码国产在线，如羥自由基我要看久久精品黄色片，超氧陰離子等；CO作為一種氣體信使分子日韩无码肏逼，在神經(jīng)元信息傳遞和血管緊張度調(diào)節(jié)中起重要作用亚洲一区二区三区激情在线观看，HO作為產(chǎn)生內(nèi)源性CO的主要酶系統(tǒng)日益受到關(guān)注。

Glioma is still one of refractory disease in the neurosurgical field; the development of new primary and adjuvant treatment is vital. Recently, the gene therapy of glioma is developed rapidly and there are many methods about the gene therapy that include: suicide gene therapy, immunologic gene therapy, drug resistangce gene therapy, angiostatin gene therapy and so on. The sucide gene therapy is the most potential approach of antitumer, these nonmammalian genes encode enzyme that convert nontoxic prodrugs into highly toxic metablites. Cells transfected with suicide genes are targeted for specific negative selection, witch can be induced by administrtion of the corresponding produg. Among the enzyme/produg combinations, two of the best characterized system are herpes simplex virus thymidine kinase /ganciclovir and Escherichia coli cytosine deaminase /5-flourocytosine (5-FC). The formor can convert the antiviral nucleoside analogs acyclovir , ganciclovir to their nucleoside monophosphate derivatives, the monophosphate forms are subsequently phosphorylated by endogenus cellular kinases to triphosphates, these molecules are potent inhibitors of DNA synthesis.

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Salicylaldehyde and its derivatives often produce a formation of Schiff base metal chelate. They are special anti-cancer, anti-inflammatory, sterilization matters. At the same time because of therir structure similar to porphyrin ring, they can set of oxygen and simulated biological effect, So many scientist take interest in this field.

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In this study, the variable region genes of three stem McAb 1A〓-D〓, 2F〓-B〓 and 2E〓-E〓 against metal-bound tetrapeptide were joined into a single chain antibody with a linker respectively. And insert 39bp fragment of N-terminal CGRP to C-terminal of heavy chain and constitute V〓linker-V〓-CGRP single chain genes.

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Comparing with chicken egg-white lysozyme which is widely used in clinic, hLYZ has many advantages: it is a natural protein in human body, so it is safe and harmless when used as a drug; its bioactivity to kill bacterium is three times higher than chicken egg-white lysozyme, and its thermal stability is much higher, too; it also has many other specific important functions which are not related with its catalysis, such as anti-virus and anti-tumor and improving immunization.

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The PyNPase could also catalyse the synthesis of 5-fluorouridine from 5-fluorouracil and uridine, and 47% uridine was converted to 5-fluorouridine.

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anticatalyst：抗催化劑

抗刺槐毒素antirobin | 抗催化劑anticatalyst | 抗催化酶anticatalase

hammerhead ribozyme：錘頭狀核酶

抗HPV16 e6/E7的錘頭狀核酶(hammerhead ribozyme)已被證實(shí)可在某種程度上抑制體外培養(yǎng)的腫瘤預(yù)防細(xì)胞抑郁的生長. 另一種發(fā)夾狀核酶(hairpin ribozyme)則被認(rèn)為是極具潛力的抗病毒咨詢制劑. 設(shè)計合成的抗HPV16的發(fā)夾狀核酶在體外試驗中具有很高的催化裂解靶mRNA的效率;

knotting：打結(jié)

DNA的拓?fù)?導(dǎo)構(gòu)體分打結(jié)(knotting)與解結(jié),連環(huán)(catenating)與解連環(huán)、超 螺旋與松弛等幾種類型. 70年代初,Wang首先從細(xì)菌中發(fā)現(xiàn) 并分離出抗撲異構(gòu)酶Ⅰ(TopoⅠ),證實(shí)它能夠催化DNA拓?fù)?異構(gòu)體之間的相互變換,是分子量約為100kD的單般入蛋白.

anticatalase：抗過氧化氫酶, 抗催化酶

anticarious | 抗齲的 | anticatalase | 抗過氧化氫酶, 抗催化酶 | anticatalyst | 抗化劑, 反催化劑

anticatalase：抗催化酶

抗催化劑anticatalyst | 抗催化酶anticatalase | 抗代謝物antimetabolite

terpenoid：萜

植物抗毒素(Phytoalexin)是當(dāng)植物受外界病原菌侵染時合成的具有抗病原菌活性的一類低分子量抗菌化合物,如類黃酮(flavonold)和類萜(terpenoid)等,催化其生化反應(yīng)的酶被稱為防衛(wèi)蛋白.