A independent life individuality is finally formated from singlecell ferti1ized egg throngh egg cleavage97干综合在线、morula stage、blastocoele stage中文字幕一区二区、protogaster stage and orange etc, and concerned in major basal questions of development and gene expression.

單細(xì)胞的受精卵通過卵裂在线精品自拍第一页、桑椹胚、囊胚日韩高清1区2区3区、原腸胚及器官的發(fā)生等久久一纵黄片，最后形成一個(gè)能獨(dú)立生活的個(gè)體，涉及發(fā)育和基因表達(dá)的許多基本問題日韩在线精品色欲视频播放网站免费。

By statistic analysis, there were significant differences with the average cell number of each morulae, early blastula and blastula between in vivo and in vitro, there were no differences with the hatching of blastula between in vivo and in vitro.

經(jīng)統(tǒng)計(jì)分析體內(nèi)桑椹胚A片视频在线一区二区、早期囊胚、囊胚的每一胚胎平均細(xì)胞數(shù)與體外桑椹胚好男人社区影视在线、早期囊胚欧美日韩va视频、囊胚的每一胚胎平均細(xì)胞數(shù)有顯著差異；擴(kuò)張囊胚的體內(nèi)欧美自拍日韩经典，體外每一胚胎平均細(xì)胞數(shù)沒有顯著差異女人天堂天天色。

Results:LIF improved the development rate of early embryo at the concentration of 0.1,1 and 10ng/ml(P.05).The enhanced effects of LIF were significant for morula and blastocyst stage,not for 1～8 cell stage of embryos.Conclusion:LIF may improve the development of early mouse embryos at the appropriate concentration.

結(jié)果：①0.1ng/ml、1ng/ml和10ng/ml濃度的LIF對小鼠早期胚胎的發(fā)育和胚泡的形成有促進(jìn)作用97碰碰碰免费人妻，0.01ng/ml和100ng/ml濃度的LIF對胚胎的發(fā)育無明顯影響超碰夜晚人人爽人人爱；②LIF對小鼠早期胚胎發(fā)育的影響主要發(fā)生于桑椹胚和胚泡期，對1～8細(xì)胞期胚胎無明顯影響手机看片日韩av人人爽人人操。

There are significantly differences on H_2O_2 levels between embryos in vivo and cultured in vitro on the early 2-cell stage and late 2-cell stage, but there are no significantly differences among embryos those on the 4-cell stage, morula and blastula using SPSS statistics software.

我們測量的結(jié)果是體內(nèi)2-細(xì)胞早期十八岁以下免费观看黄色网站、2-細(xì)胞晚期、4-細(xì)胞期影音先锋图片资源站、桑椹胚一级特黄大片久久久久、囊胚胚胎的EB熒光密度分別是52.92，50.77国产三级国产精品专区，48.84乱女伦一级毛片，39.96和30.36；體外2-細(xì)胞早期91涩涩社区、2-細(xì)胞晚期国产精品免费专区、4-細(xì)胞期、桑椹胚亚洲无码黄色视频无码、囊胚胚胎的EB熒光密度分別是74.85国产女人人人操，82.62，82.98尤物在线观看视频，81.28和54.09国产精品永久免费啪啪啪。

Studies on mouse nuclear transfer with compacted morula blastomeres as donors In this study, C57BL/6 mouse morulae blastomeres and Kunming white mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different sucrose concentration on the manipulation time of nuclear transfer, and the in vitro development of reconstructed embryos.

以致密桑椹胚卵裂球?yàn)楣w克隆小鼠的研究該研究以C57BL/6 小鼠致密桑椹胚卵裂球?yàn)楣w日本a在线网址、昆明白小鼠MⅡ期卵母細(xì)胞為受體，研究了不同蔗糖濃度對核移植操作時(shí)間及重構(gòu)胚體外發(fā)育的影響国产精品自拍在线。

Here were expression of TGFαand EGFR mRNA in unmatured oocyte, matured oocyte, embryo and the expression of TGFαand EGFR in matured oocytes were comparatively more abundant than that in unmatured oocytes.

GFαmRNA的量在8細(xì)胞期下降到最低水平成年人的网站，并隨著胚胎基因組的激活胚胎開始轉(zhuǎn)錄TGFα，使TGFαmRNA表達(dá)總量保持在一定水平91在线播放，持續(xù)到桑椹胚期欧美一级A片大全免费观看；EGFR mRNA的量在胚胎發(fā)育到桑椹胚期下降到最低，而囊胚期胚胎中的整體表達(dá)量又上升东北老妓女叫床脏话对白视频。

With the increase of the 1254 dosages, in the further development process of 2-cell embryo, there still occurred the development relayed and degeneration, so that the development rate of morulae and blastocysts decreascd significantly.

在受精卵的進(jìn)一步發(fā)育中亚洲欧美一区二区蜜臀图片，隨著公鼠染毒劑量的增加，發(fā)育到桑椹胚和囊胚的比例顯著降低直接观看黄网站免费裸体赌场网站，發(fā)育延遲胚及發(fā)育中退化變性胚比率顯著升高爱情岛亚洲成年永久网站。

This experiment object was 1-cell embryo of KUNMING mouse , ROS was added in different developmental phases through basal medium with CZB medium, cultured to morulae, early blastula, blastula and the hatching of blastula, observed the change of cell number and cleavage index of embryo.

本試驗(yàn)以昆明小鼠1-細(xì)胞期胚胎為實(shí)驗(yàn)對象，以CZB培養(yǎng)液為基礎(chǔ)液在不同發(fā)育階段添加外源性ROS美女操白浆网站，培養(yǎng)至桑椹胚日韩一级毛一欧美一级毛免费、早期囊胚、囊胚毛片高清视频在线看免费观看、擴(kuò)張囊胚階段爱搞无码在线，分別觀察胚胎的胚細(xì)胞數(shù)、分裂指數(shù)的變化国产网友愉拍精品视频。

In the experiment, the FSH+LH combinations were adopted to superovulate KM mice to ascertain the proper phase to gain morulae and blastocysts, three ways were adopted to split the morulae and blastocysts, demi-embryos were cultured in D-PBS, mTCM199, CZB, mM16 added with FCS or MS of different concentration, and transplantation of the blastocysts derived from demi-embryos was performed.

本試驗(yàn)以昆明白小鼠為實(shí)驗(yàn)動物最大黄色网站一级在线，試采用FSH和LH進(jìn)行超排處理，確定了該處理程序下獲取桑椹胚久久免费大黄片、囊胚的恰當(dāng)時(shí)間一a在线视频插进去了，比較了三種方法對桑椹胚和囊胚分割的效果，采用了添加不同濃度FCS最近中文字幕免费MP4视频、MS的4種培養(yǎng)基對分割胚進(jìn)行體外培養(yǎng)在线看黄不卡免费AV，并進(jìn)行了半胚移植。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分為4個(gè)部分800avcvm，第一部分對影響豬卵母細(xì)胞體外成熟和孤雌激活后胚胎分裂的幾個(gè)重要條件進(jìn)行了比較研究，確立了一種較好的培養(yǎng)方法：與顆粒細(xì)胞共培養(yǎng)免费黄色在线观看网站，找到了一種適合豬卵母細(xì)胞體外成熟的培養(yǎng)基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸国产精品黄色在线观看，成熟率和分裂率分別為(86.7±3.35)%和(86.3±4.16)%，國外報(bào)道的最高成熟率為(85.7±4.1)%人人做人人叼嘿；第二部分對豬卵母細(xì)胞孤雌激活的化學(xué)方法進(jìn)行了研究色综合久久加勒比中文字幕，確立了化學(xué)激活豬卵母細(xì)胞的兩種最佳方法：1將成熟的去卵丘顆粒細(xì)胞的MII期卵母細(xì)胞用20μmol/Lionomycin作用30min，再將卵母細(xì)胞培養(yǎng)于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培養(yǎng)液中国产91在，卵裂率和桑囊胚發(fā)育率達(dá)到(76.7±7.6)%和(37.1±6.4)%2將成熟的去卵丘顆粒細(xì)胞的MII期卵母細(xì)胞在200μM/L的Thimerosal中處理20min国产一级美女黄片A，再與8mM的DTT共孵育30min老妇女性较大毛片视频A片那里有，卵裂率和桑/囊胚形成率為(81.0±2.8)%和(39.6±2.7)%；第三部分對孤雌激活胚胎的培養(yǎng)條件進(jìn)行了研究jizz国产视频，確立了一種最佳的胚胎培養(yǎng)條件：在SOF簡單培養(yǎng)基中添加顆粒細(xì)胞進(jìn)行前3天的培養(yǎng)五月草视频，然后轉(zhuǎn)入添加胎牛血清的NCSU—23培養(yǎng)基并和輸卵管上皮細(xì)胞進(jìn)行后期的培養(yǎng)，其桑椹胚和囊胚的發(fā)育率為(59.5±3.2)%aA电影黄色电影网；第四部分研究了IGF-I

morula：桑椹胚

oss)基因異常必定能達(dá)到桑椹胚(morula)、囊胚(blastula)與著床的階段滋養(yǎng)母細(xì)胞(trophoblast)的增生壓倒了雙層滋養(yǎng)母細(xì)胞更多黏液物質(zhì)(mucoid material)與水腫血管的壁 內(nèi)緣表現(xiàn)出滑膜(synovium)更甚于上皮只見于 CHM 與 PHM ,

morula：[動]桑椹胚

movie theater 電影院 | morula [動]桑椹胚 | QR queen's rook (國際象棋中的)后翼車

morula stage：桑椹胚期

桑椹胚 morula | 桑椹胚期 morula stage | 囊胚形成 blastulation

morulation：桑椹胚形成

morula 桑椹胚 | morulation 桑椹胚形成 | mosaic 嵌合體

morulation：桑葚胚形成 桑椹胚形成

morulardelamination桑椹胚分層 | morulation桑葚胚形成 桑椹胚形成 | moruloid桑椹樣的

morulation：桑葚胚形成

morular 桑椹胚的 | morulation 桑葚胚形成 | morulation 桑椹胚形成

amphimorula：兩極桑椹胚

\\"兩性融合\\",\\"amphimixis\\" | \\"兩極桑椹胚\\",\\"amphimorula\\" | \\"雙經(jīng)綱\\",\\"AMPHINEURA\\"

morular：桑椹胚的

morulamesoblast桑椹胚中胚層 | morular桑椹胚的 | morulardelamination桑椹胚分層

moruloid：桑椹樣的

morulation桑葚胚形成 桑椹胚形成 | moruloid桑椹樣的 | Moruloidea桑椹綱

morulae：(復(fù)數(shù))桑椹胚

morula 桑椹胚 | morulae (復(fù)數(shù))桑椹胚 | mosaic 鑲嵌物;鑲嵌型;花葉的