The results showed that 1 there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 μg/mL) for 0.5 h and oocytes that were incubated in colchicine (0.4 μg/mL) for 1 h, and the rate of cytoplast protusion can be 85.4% while the rate of cytoplast enucleation is 100%. 2 There was no significant difference in oocyte enucleation between oocytes treated with medium containing 0.2 μg/mL colchicine for 0.5 h and oocytes treated with medium containing 0.4 μg/mL colchicine for 0.5 h. 3 A maturation time of 18–23 h did not affect the rates of cytoplast protusion and enucleation by chemically assisted enucleation, whereas the rate of enucleation of oocytes by blind enucleation was found to decrease with a prolonged incubation time. 4 The development rates of reconstructed embryos could not be influenced by these two enucleation methods, increased from oocytes matured for 21–23 h.

結(jié)果表明： 1 卵母細胞在0.4 mg/mL的秋水仙素溶液中分別孵育0.5 h和1 h精品黄色A片在线观看，胞質(zhì)突起率和去核率沒有顯著的差異清纯美女被操，突起率可高達85.4%99pom国产熟女，去核率達到100%很色很黄很污的网站在线看； 2 0.2 mg/mL或0.4 mg/mL秋水仙素溶液將卵母細胞處理0.5 h，對去核效果沒有顯著影響肏丝袜屄影片； 3 對于體外成熟18~23 h的卵母細胞日韩mv在线看成人网站，隨著成熟時間的延長，盲吸法的去核率降低二级免费黄片国产，但沒有影響秋水仙素誘導(dǎo)胞質(zhì)突起的比率和去核率yw国产在线视频在线观看； 4 兩種去核方法對重構(gòu)胚的發(fā)育沒有產(chǎn)生顯著影響，但成熟21~23 h卵母細胞重構(gòu)胚囊胚的發(fā)育率顯著高于成熟18~20 h卵母細胞重構(gòu)胚囊胚的發(fā)育率一级A片岛國。

The snail was found to have an indirect developmental type in the early development. The fertilized eggs were oval-shaped and discoidal cleavage. Embryonic development stages were divided into cleavage, blastula, gastrulae, trochophore, and intra-membrane veliger. Larval development stages included veliger, late veliger and crawling larvae metamorphosed to juvenile.

結(jié)果表明：黃口荔枝螺卵囊高度平均為7.33 mm处女开破视频，卵粒數(shù)量平均為165粒；黃口荔枝螺的早期發(fā)育屬間接發(fā)生型，受精卵呈橢圓形嗯好深啊用力哦啊视频，卵裂為盤狀卵裂中国三级黄色片18岁美女全体逼；在卵囊內(nèi)，胚胎發(fā)育包括卵裂期黄色高清无码在线、囊胚期特级欧美婬片免费高直播播放、原腸胚、膜內(nèi)擔(dān)輪幼蟲视频:黄片公司、膜內(nèi)面盤幼蟲欧美黄色成年网站视频；幼蟲發(fā)育包括面盤幼蟲、后期面盤幼蟲和匍匐幼蟲黄色工厂在线看。

The results of three eggapparatus and two egg apparatus cause the fact that the frequency of twin is higherthan that of triple embryos.

三卵卵器結(jié)構(gòu)胚囊"和"二卵卵器結(jié)構(gòu)胚囊"的受精情況造成了APⅣ的2胚頻率遠高于3胚的麻豆A片在线观看。"

Objective To establish an animal model of Capillaria hepatica.

建立肝毛細線蟲的動物模型。將肝毛細線蟲的孕胚卵經(jīng)口注入大鼠和家貓人人草网。

Studies on mouse nuclear transfer with compacted morula blastomeres as donors In this study, C57BL/6 mouse morulae blastomeres and Kunming white mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different sucrose concentration on the manipulation time of nuclear transfer, and the in vitro development of reconstructed embryos.

以致密桑椹胚卵裂球為供體克隆小鼠的研究該研究以C57BL/6 小鼠致密桑椹胚卵裂球為供體视频一区二区久久91、昆明白小鼠MⅡ期卵母細胞為受體，研究了不同蔗糖濃度對核移植操作時間及重構(gòu)胚體外發(fā)育的影響一级婬片又大又长又粗。

Each embryo were inoculated by a dose of 1×104 pure sporozoites was injected with testing Bt crystal proteins before 24 hours. The varying oocyst inhibition rates of embryos brought about by Bt crystal proteins that were given at various doses were used as a criterion for determining the activity of Bt crystal proteins.

每一雞胚接種1×104個子孢子Ⅴ片视频免费在线观看，于感染前24h注入蘇云金桿菌晶體蛋白，以不同蘇云金桿菌晶體蛋白對雞胚卵囊所產(chǎn)生的抑制率的差異男女激情网站，判定蘇云金桿菌的活性国产精品资源网在线观看。

These observations show that reprogramming is easier in interspecific embryos reconstructed with ES cells than that reconstructed with somatic cells, and that ES cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. Oocytes were reconstructed with outbreeding Kunming albino mouse ES cells and enucleated rabbit oocytes, and the effects of the passages of ES cells and 6-DMAP on the development of interspecific reconstructed oocytes were analyzed. The interspecific reconstructed ES-rabbit oocytes were activated either by combined two set electric pulses and 6-DMAP or by two set electr

以上結(jié)果顯示，6－DMAP能增加胚胎干細胞異種重構(gòu)卵的卵裂率色综合91，對重構(gòu)卵囊胚的發(fā)育率影響不大人人爽人人看人人硬；高培養(yǎng)代數(shù)和低培養(yǎng)代數(shù)的胚胎干細胞用于異種核移植不影響異種重構(gòu)卵的卵裂率和囊胚發(fā)育率；用胚胎干細胞作為供核細胞噜噜嘿在线无码视频；比體細胞作為供核細胞所構(gòu)建的異種重構(gòu)胚更容易進行重編程在线等免费看黄片，并且胚胎于細胞指導(dǎo)異種克隆胚胎正常發(fā)育的能力強于體細胞。

Different strains have different reaction to the delay of oviducts in body after animal death. After 10min delay C57BL/6 mouse oocytes have death rate of 56.5%,significantely higher than Kunming mouse (47.6%);Spontaneous activation rates were respectively 13.3% and 46.0%, C57BL/6 were obviously lower than Kunming mouse.4. The oviducts were obtained after being delayed 5min 24h after the mice were injected with hCG. The oocytes were cultured in CZB. About 81.1% occurred spontaneous activation, evidently lower than parthenogenetic rate (96.4%) with SrCl_2. Spontaneous activable oocytes had high cleavage rate(93.2%) and 4-cell rate(87.3%). However, spontaneous activable oocytes had blastula development rate(18.7%) as low as parthenogenetic oocytes by SrCl_2(22.9%).

不同品系小鼠卵母細胞對輸卵管在體內(nèi)滯留產(chǎn)生的反應(yīng)不同欧美综合色网站，滯留10min C57BL/6系小鼠卵母細胞死亡率為56.5%美国少妇a级，顯著高于昆明鼠(47.6%)；自發(fā)激活率分別為13.3%和46.0%黄色视频在线免费观看无码高清强奸，C57BL/6系小鼠顯著低于昆明鼠亚洲第一视频网。4.hCG后24h體內(nèi)滯留5min卵母細胞在CZB中培養(yǎng)自發(fā)激活率為81.1%，顯著低于SrCl_2孤雌激活率(96.4%)www.tzbeta.com；自發(fā)激活的卵母細胞有較高的卵裂率(93.2%)和4-cell比率(87.3%)伊人久久综合精品永久图片，但囊胚率(18.7%)較低，同卵齡的卵母細胞經(jīng)SrCl_2孤雌激活囊胚發(fā)育率為22.9%国产嫖妓一区精品视频，差異不顯著av九一。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分為4個部分欧洲人与动牲交Α欧美精品，第一部分對影響豬卵母細胞體外成熟和孤雌激活后胚胎分裂的幾個重要條件進行了比較研究，確立了一種較好的培養(yǎng)方法：與顆粒細胞共培養(yǎng)亚洲欧美中文日韩V日本，找到了一種適合豬卵母細胞體外成熟的培養(yǎng)基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸日韩性色生活免费观看，成熟率和分裂率分別為(86.7±3.35)%和(86.3±4.16)%，國外報道的最高成熟率為(85.7±4.1)%大伊人一区在线视频；第二部分對豬卵母細胞孤雌激活的化學(xué)方法進行了研究99er在线视频，確立了化學(xué)激活豬卵母細胞的兩種最佳方法：1將成熟的去卵丘顆粒細胞的MII期卵母細胞用20μmol/Lionomycin作用30min，再將卵母細胞培養(yǎng)于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培養(yǎng)液中乱色熟女一区二区免费视频，卵裂率和桑囊胚發(fā)育率達到(76.7±7.6)%和(37.1±6.4)%2將成熟的去卵丘顆粒細胞的MII期卵母細胞在200μM/L的Thimerosal中處理20min欧美一级A片直播，再與8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率為(81.0±2.8)%和(39.6±2.7)%国产精品原创综合；第三部分對孤雌激活胚胎的培養(yǎng)條件進行了研究啪啪啪免费无码视频，確立了一種最佳的胚胎培養(yǎng)條件：在SOF簡單培養(yǎng)基中添加顆粒細胞進行前3天的培養(yǎng)，然后轉(zhuǎn)入添加胎牛血清的NCSU—23培養(yǎng)基并和輸卵管上皮細胞進行后期的培養(yǎng)免费看的毛片大全网站，其桑椹胚和囊胚的發(fā)育率為(59.5±3.2)%wwjizz国产；第四部分研究了IGF-I

Following chemical activation, blastocysts rate of the treated oocytes was similar to untreated oocytes.8 Following fertilization, however, few oocytes inhibited with CHX developed into morulae/blastocysts, due to a high incidence of polyspermy.9 Cortical granule migration occurred during inhibition, but CHX inhibition impaired CG migration, significantly no oocytes inhibited by CHX completed CG migration after maturation.10 CHX inhibition had no effects onα-microtubles and microfilaments of goat oocytes.

抑制24h轉(zhuǎn)為正常培養(yǎng)24h，不影響卵母細胞的成熟和孤雌激活能力国产福利院精品，并且CHX抑制后再成熟的卵母細胞經(jīng)孤雌激活發(fā)育到囊胚的比例與對照組卵母細胞相似国产熟睡乱子乱A片。8、體外受精后欧美性爱视频在线观看鲁片，CHX抑制后再成熟卵母細胞的多精受精現(xiàn)象顯著增加最新国产小视频，發(fā)育到桑椹胚/囊胚的比例顯著低于對照組。9色99色网站、CHX抑制過程中皮質(zhì)顆粒仍能發(fā)生遷移，但是CHX抑制會對皮質(zhì)顆粒的遷移造成不可恢復(fù)的損傷97人人草人人，使再成熟的卵母細胞內(nèi)皮質(zhì)顆粒不能完全遷移A片视频网站在线免费观看。10、CHX抑制對山羊卵母細胞α-微管和微絲都沒有影響肏屄在线免费视频，無論是抑制后處于生發(fā)泡期的卵母細胞精品浮力影院一区二区，還是抑制后再成熟的卵母細胞，微管和微絲的分布都與對照卵母細胞相似一av天堂。

addled egg：死胚蛋,臭蛋,無胚卵

additve 添加劑 | addled egg 死胚蛋,臭蛋,無胚卵 | ADDPSS, advanced digital power system simulator 電科院全數(shù)字電力系統(tǒng)仿真器

blastomere：(卵)裂球

卵裂產(chǎn)生的細胞稱為囊胚細胞或卵裂球(blastomere). 卵裂球只是數(shù)量增多,每個卵裂球沒有生長,卵裂球緊挨在一起,總體沒有增大. 第一次卵裂和第二次卵裂都是縱向分裂,但兩者的分裂面相互呈直角,把受精卵垂直分裂成4個體積相同的卵裂球.

blastulation：囊胚形成

這一過程是在親本的生殖腺中進行.由生殖腺上皮形成1.卵裂(cleavage)卵受精后很快即開始細胞分裂,這就是卵裂,卵裂(meridional cleavage),即彼此呈直角平行于卵的極軸(pol-ar axis)2.囊胚形成(blastulation)在卵裂形成一定數(shù)量的分裂球之后,

endoscopic：胚的頂端向藏卵器的基部的

胚抽出液 embryo extract | 胚的頂端向藏卵器的基部的 endoscopic | 胚的發(fā)育 embryonic development

cleavage polyembryony：卵割多胚, 裂生多胚現(xiàn)象

cleavage plane | 解理面 晶體的裂開面 | cleavage polyembryony | 卵割多胚, 裂生多胚現(xiàn)象 | cleavage product | 分裂產(chǎn)物

cleavage polyembryony：卵裂多胚性

卵裂多胚 cleavage polyembryo | 卵裂多胚性 cleavage polyembryony | 卵裂紡錘體 cleavage spindle

simple polyembryony：簡單多胚[現(xiàn)象]

裸子植物常具多胚現(xiàn)象,多胚現(xiàn)象(phlyembryony)的產(chǎn)生有兩個途徑:一是簡單多胚現(xiàn)象(simple polyembryony),由一個雌配子體上的幾個頸卵器同時受精,形成多胚;另一是裂生多胚現(xiàn)象(cleavage polyembryony),僅一個卵受精,但在發(fā)育過程中,

unequal coeloblastula：不等卵囊腔胚

04.0241 有腔囊胚 coeloblastula | 04.0242 不等卵囊腔胚 unequal coeloblastula | 04.0243 原腸胚 gastrula

ovigerm：原卵 胚卵

oviger 攜卵足 | ovigerm 原卵 胚卵 | ovigerous 產(chǎn)卵的

Ovigerm Stage：胚卵期

胎兒期 Fetal Stage | 胚卵期 Ovigerm Stage | 胚胎期 Embryo Stage