However, commercially available CPB purified from porcine pancreas was very expensive, and was not totally free of other proteases, such as trypsin and chymotrypsin.

到目前為止，商業(yè)途徑獲得的羧肽酶B 主要從豬胰臟中提取欧美性多多，使得產(chǎn)品價(jià)格昂貴扒开双腿疯狂进出爽爽爽网站，同時(shí)也不可避免地混雜有其它蛋白酶，如胰蛋白酶性xxxx淫乱，胰凝乳蛋白酶人人操人人湿人人干。

In the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic S helix of AOX. were synthesized by solid-phase method. The polypeptide was coupled to a -chymotrypsinogen, and the antibodies were obtained through immunizing rabbit by injecting this complex.

第一部分，我們按照交替氧化酶(Alternative Oxidase日韩色情精品一区-AV，AOX)位于線粒體內(nèi)膜外側(cè)親水區(qū)S螺旋的氨基酸序列欧美一级在线观看欧美大片，用固相法合成由12個(gè)氨基酸殘基組成的多肽，將此多肽與α-牛胰凝乳蛋白酶原A相連一级A片无码性区，用作半抗原1024在线看片你懂的，免疫家兔制備人工合成12肽的抗體。

The results demonstrated that the measured molecular weight of phycocyanin was 37.715Kd, and consisted of two peptides which were similar with their molecular weight and amino acid sequences.4 The comformation of phycocyaninPhycocyanin high structure was investigated, as the result of circular dichroism spectrum and Fourier transformation infared spectrum shown, the secondary structure of NSPC was mainlyα-helix structure and the content ofβ-shee or coil was very small.

根據(jù)氨基酸自動(dòng)分析儀測(cè)定的氨基酸組成和精確分子質(zhì)量計(jì)算出NSPC分子總共有331個(gè)氨基酸殘基免费观看视频精品免费观看，分別是由40個(gè)Asp亚洲一区二区三区a片、22個(gè)Thr、18個(gè)Ser中文字幕人妻少妇aⅴ一区二区、22個(gè)Glu一区二区亚洲黄色网站、1個(gè)Gly欧美精品AAAAAAA、31個(gè)Ala、27個(gè)Cys中国农村第一黄片视频、17個(gè)Val久久精品中文字幕乱码…、13個(gè)Ile、22個(gè)Leu热久久中文字幕少妇、5個(gè)Tyr免费一级毛片视频,、7個(gè)Phe、7個(gè)Lys亚洲色淫淫网、24個(gè)His国产永久视频、51個(gè)Arg、14個(gè)Pro国产精品久久久狼友、1個(gè)Met和9個(gè)Trp組成欧美一级夜夜爽老妇。胰蛋白水解酶分別酶解NSPC-1和NSPC-2后，分別采用MALDI-TOF-MS分析男人天堂网址在线，其肽指紋譜圖顯示胰蛋白酶可將NSPC-1和NSPC-2水解成大小不等的多條肽段手机看片日韩精品视频在线，質(zhì)量數(shù)在800以上的NSPC-2有11段，即水解的肽質(zhì)量分別為899.54黄色A片大全、1454.77黄片操美女、1544.9、1683.87性插进去啪啪视频呢、1803.93国产在线精品国自产拍影院同、1811.91、1987.92欧美性爱与欧美性爱、2003.90www.c黄、2536.26、2915.42和3492.72欧美老妇乱惀。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各種純化方法激情你懂的，將離子交換層析、親和層析和疏水層析三種分離純化法相結(jié)合国际一级黄色片，建立了激肽釋放酶呦福利导航、胰蛋白酶和糜蛋白酶三酶的聯(lián)產(chǎn)工藝：胰酶用pH4.5醋酸緩沖溶液提取后，粗提液直接上DEAE-瓊脂糖快膠柱吸附平衡緩沖液：0.01mol／LNaoAc-HoAc緩沖液(pH4.5青春草在线视频一区二区三区，洗脫緩沖液：0.01mol／LNaoAc-HoAc緩沖液(pH4.5)含0.2～0.35mol／LNaCl分兩步洗脫真人一级黄色视频，收集激肽釋放酶的洗脫峰；DEAE-瓊脂糖快膠的未吸附液上親和層析柱分批吸附平衡緩沖液：0.01mol／LTris-HCl緩沖液(pH7.5亚洲是图综合，洗脫液：0.5mol／LNaCl溶液欧美一级黄色视频A片，一次洗脫，收集胰蛋白酶洗脫峰；最后欧美浓毛老太乱人伦视频在线，親和層析未吸附液用30％～80％硫酸銨分級(jí)鹽析處理A片在线观看网站免费视频，沉淀溶解后用上苯基—瓊脂糖快膠吸附平衡緩沖液：0.01mol／LNaAc-HAc緩沖液(pH4.5含1mol／L(NH_4)_2SO_4，洗脫緩沖液：0.01mol／LNaAc-HAc緩沖液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4中文字幕黄色，分兩步洗脫国产女优视频，收集糜蛋白酶的洗脫峰。

PACAP is a pleiotropic neuropeptide that belongs to the secretin/glucagon/vasoactive intestinal peptide family.

垂體腺苷酸環(huán)化酶激活肽是多效性的神經(jīng)肽国产AV色8X，屬于腸促胰液素/胰高血糖素/腸血管活性肽家族亚洲综合AⅤ一区二区三区导航。

We make chloroacetylaion to N-terminal of Asp, and then convert this compound to GD dipeptide by aminolysis. Followed the lipase -catalyzed peptide synthesis of RGD in aqueous water-miscible organic cosolvent is studied. We set up a new efficient reaction method to synthesize RGD tripeptide. The problem that peptide containing polar amino acid can not be synthesized readily in organic solvent was solved. We offer a new experimental proof for PPL-catalyzed peptide synthesis and make a more general analysis for the effect of some factors in theory.

采用氯乙酰基保護(hù)天冬氨酸国产狼友精品免费视频一二三四区，然后進(jìn)行氨解生成GD二肽女生啪啪视频，利用豬胰脂肪酶催化合成RGD三肽，著重解決了親水氨基酸/肽片段底物的溶解性與合成產(chǎn)率低的問題欧美一级久久，建立了一種新的有效合成RGD三肽的反應(yīng)模型99久久99国产免热在播，并探索解決了極性較強(qiáng)的氨基酸的酶促肽合成的問題欧美日韩国产在线mv，對(duì)脂肪酶催化合成肽的影響因素從理論上作了較全面的分析www.taohongbei.com，為肽片段的縮合反應(yīng)提供了一種新思路。

Methods : The levels of fasting blood glucose and fast plasma insulin, the activities of superoxide dismutase and glutathione peroxidase and level of lipid peroxidate were measured in the pancreas, heart and liver of normal and streptozotocin-induced diabetes-prone mice exposed to multiple stressors for 6 weeks. Results : The FBG level in STZ mice was elevated by stress, while the FINS level was not increased.

用鏈脲佐菌素復(fù)制糖尿病傾向小鼠模型亚洲二三区，予以6周慢性多相性應(yīng)激一级A片一级毛片一级人6，檢測(cè)其空腹血糖、空腹胰島素水平和胰谁有黄色网站久久久、心日韩主播欧美精品视频、肝組織超氧化物歧化酶、谷肽甘肽過氧化物酶活性及脂質(zhì)過氧化物含量国产小视频网址。

Full enzymatic synthesis of N-protected Leu-enkephalin was successfully achieved via 3+2synthetic route by α-chymotrypsin and thermolysin, and the Z-protected Leu-enkephalin phenylhydrazine was obtained in tert-amyl alcohol by thermolysin for the first time.

以3+2的合成路線使用α-胰凝乳蛋白酶和嗜熱桿菌蛋白酶完成了N-保護(hù)的亮-腦啡肽的酶法全合成欧美偷操，并在第三戊醇中首次以嗜熱桿菌蛋白酶為催化劑得到了Z-保護(hù)的亮-腦啡肽酰苯肼衍生物。

The microheterogeneity and tryptic fragments of glycoprotein recombinant human erythropoietin were analyzed by high performance capillary electrophoresis.

用高效毛細(xì)管電泳測(cè)定了重組人促紅細(xì)胞生成素的微多相性AV网在线www观看、肽圖欧美福利二区，同時(shí)采用毛細(xì)管電泳質(zhì)譜聯(lián)用技術(shù)鑒定了部分非糖基化胰酶消解片段和O－126糖基化肽的結(jié)構(gòu)。

The results showed that: 1 SOD and GSH-PX activity of hepatopancreas and serum were significantly or extremely significantly increased when 5% or 10% alfalfa meal was added to the diet, whereas the MDA content of the two groups decreased. 2 The concentration of TC and TG in serum, hepatopancrac and muscle dropped as the amount of alfalfa meal increased.

結(jié)果顯示免费看色网站，1添加5%和10%的苜蓿草粉能顯著或極顯著提高黃河鯉魚血清和肝胰臟中超氧化物歧化酶和谷胱甘肽過氧化物酶活力人人看人人摸人人干，同時(shí)也能顯著或極顯著降低丙二醛含量，說明苜蓿草粉能有效地提高黃河鯉魚的抗氧化能力欧美日韩xxxx。2血清韩国三级免费在线观看、肝胰臟和肌肉中總膽固醇和甘油三酯含量都隨著苜蓿草粉添加量的增加依次降低。

erepsin：腸肽酶

[成分]主要含蛋白質(zhì)国产一级黄色操逼片、脂肪、甲殼質(zhì)、糖類及蛋白質(zhì)分解酵素. 蛋白質(zhì)分解酵素為腸肽酶(erepsin)及胰元酶(trypsin).

leucine aminopeptidase：亮氨酸氨基肽酶

有時(shí)患者血清中胰蛋白酶无码中文字幕A片在线、亮氨酸氨基肽酶(leucine aminopeptidase)亚洲人妻中文、淀粉酶等均增高,但由于其結(jié)果均不恒定,在臨床診斷上不常應(yīng)用. 有報(bào)告約80%~90%患者血清CEA檢出陽性,但因CEA的特異性不強(qiáng),亦不能作為診斷本癌的根據(jù). 術(shù)前可以進(jìn)行ERCP胰管細(xì)胞刷片或活檢;

pancreozymin：促胰酶素

CCK-PZ:是由十二指腸及空腸粘膜提取的一種肽類物質(zhì),它可刺激胰腺分泌大量胰液,稱之促胰酶素(pancreozymin). 它與縮膽囊素(choletystokinin)相同,同時(shí)有收縮膽囊和松馳括約肌的作用,故稱為縮膽囊素-促胰酶素(cholecystokinin-pancreozymin,

pancreozymin：胰酶素

則在血漿中可以保持活性30~40小時(shí),使大鼠的胰腺炎明顯減輕.CCK-PZ:是由十二指腸及空腸粘膜提取的一種肽類物質(zhì),它可刺激胰腺分泌大量胰液,稱之促胰酶素(pancreozymin).它與縮膽囊素(choletystokinin)相同,

rennin：凝乳酶

由木瓜與鳳梨中分別抽取出來的木瓜酵素(papain)及鳳梨酵素(bromelin),均是分解蛋白質(zhì)的酵素. 藉由水解作用,它們將蛋白質(zhì)分解成較小的勝肽鏈. 其它分解蛋白質(zhì)的酵素還包括胃蛋白酶(pepsin)、胰蛋白酶(trypsin)老夫妻性交免费视频、凝乳酶(rennin)xxwww日韩在线观看视频、胰酶(pancretin)及

trypsin：胰朊酶

蛋白酶為腸肽酶(Erepsin)及胰朊酶(Trypsin). 性味咸、寒,清熱解毒国内揄拍国内孞精品人妻浪潮AV、消積食全网黄网站免费观看、健脾胃,主治消化不良、脘腹脹滿影音先锋av下载、體倦無力人人操,人人摸,人人性爱、熱病神昏、毒痢作嘔涩涩毛片在线视频看看、小兒疳積諸癥;用量1.6~3.2g,虛寒者不宜.

monomeric enzyme：?jiǎn)误w酶

1.單體酶 單體酶(monomeric enzyme)一般由一條肽鏈組成,如溶菌酶无码日韩少妇爆乳视频迅雷下载、牛胰核糖核酸酶. 單體酶種類較少,一般多是催化水解反應(yīng)的酶,相對(duì)分子量在(13~35) 103之間. 2.寡聚酶 寡聚酶(olimomeric enzyme)是由2個(gè)或2個(gè)以上亞基組成的酶,

pancreatolipase：胰酯酶

pancreatin 胰酶(制劑) | pancreatolipase 胰酯酶 | pancreokinin 胰激肽

E elastase：胰肽酶

7576 肌氨酸激酶MB同工酶 creatine kinase MB isozyme | 7580 胰肽酶E elastase | 7582 嗜中性胰肽酶E neutrophil elastase

kallikrein kinin prostaglandin system KKPGS：激肽釋放酶-激肽-前列腺素系統(tǒng)

kallidin KD 胰激肽 | kallikrein kinin prostaglandin system KKPGS 激肽釋放酶-激肽-前列腺素系統(tǒng) | kallikrein KK 激肽釋放酶