The animal experiment stage: 40 rabbits underwent corneal epithelium curettement and limbal ablation to induce limbal stem cell deficiency and were randomly divided into four groups, simple amniotic membrane group, non-frozen cells group, glycerol group and dimethyl sulfoxide group(n=10 for each group). Searching for the activity of frozen and non-frozen cells more completely by transplanting subculturing cells and observing the accomplishment of cells function.

動(dòng)物實(shí)驗(yàn)階段：將40只角膜緣干細(xì)胞損傷的模型兔隨機(jī)分成單純羊膜組狼色行动在线视频、未冷凍細(xì)胞組、DMSO保存細(xì)胞組和甘油保存細(xì)胞組亚洲综合网色五月，每組10只亚洲天堂在线网站，用傳代培養(yǎng)2周后的角膜緣上皮細(xì)胞作移植實(shí)驗(yàn)搜索欧美黄片，觀察細(xì)胞體外功能完成情況來進(jìn)一步比較深低溫保存前后角膜緣上皮細(xì)胞的活性。

We found that multipara ones had the least ocular hypotension effect during pregnancy.關(guān)鍵詞 Central corneal thickness , Corneal base curvature , Intraocular pressure , Pregnancy

本文同時(shí)密集測(cè)量8位剛生產(chǎn)完的婦女角膜中心厚度變化的情形，發(fā)現(xiàn)大部分的角膜在二到三周內(nèi)迅速變薄看片av网站在线播放，三周之后則無太大變化欧美性爱zozozoxxxx。

This experiment investigate the expression of TGF- B1 and bFGF in the rabbet corneas to find whether such expression contribute to the corneal wound healing after PRK; and compare the effects of mechanical deepithelialization with chemical deepithelialization PRK on the corneal wound healing.

裂隙燈顯微鏡下觀察角膜Haze及角膜上皮愈合、水腫消退的情況国产美女作爱视频多；光鏡下觀察角膜組織病理學(xué)變化并計(jì)數(shù)不同時(shí)間點(diǎn)角膜基質(zhì)淺層角膜細(xì)胞數(shù)量的變化A片网站在线免费观看；用逆轉(zhuǎn)錄-PCR法及免疫組化法檢測(cè)正常對(duì)照組及PRK手術(shù)組術(shù)后1天、1周无码精品视频仙踪林、1月欧美毛揉揉视频在线观看、3月不同時(shí)點(diǎn)角膜TGF—β1和bFGF的表達(dá)情況亚洲精品国产欧美一二。

Central corneal thickness measurements using ACMaster (Carl Zeiss Meditec AG, Jena, Germany) and Goldmann applanation tonometry were performed at baseline, 1 day, 1 week, and 4 weeks.

應(yīng)用角膜測(cè)厚儀測(cè)量角膜厚度和 Goldmann 壓平式眼壓劑測(cè)量眼壓国产全黄A一级毛片电瓶，分別測(cè)量基線，治療后1天www.riav7，1周www.18禁网站，四周的情況。

Methods A total of 86 eyes of successful rhegmatogenous retinal detachment with a higher encircling scleral buckle underwent A-scan and keratometer examination before surgery as well as l week,4 and 12 weeks after surgery.The refractive factors included the depth of anterior chamber,thickness of lens,axial length of eye,corneal curvature and refraction of eye were detected pre- and post-operatively.

用眼科超聲儀免费人人插操摸、角膜曲率儀免费A级不卡在线毛片、驗(yàn)光儀等對(duì)鞏膜環(huán)扎加壓眼內(nèi)嵴高為4～6 D的86例原發(fā)性視網(wǎng)膜脫離患者的86只患眼手術(shù)前1天及術(shù)后第1，4黄片免费在线看吗，12周時(shí)前房深度国产自愉怕一区二区、眼軸長(zhǎng)度、晶狀體厚度免费三级性毛毛片、眼屈光度自拍偷拍第七页、角膜曲率等進(jìn)行測(cè)量并將結(jié)果進(jìn)行比較。

There was no difference among the three groups.At the 4th week,corneal epithelial cell covering the laser section in both groups was much neoformative collagen arranging irregularly and cicatricial tissue in the anterior stromal layer.

術(shù)后一周日本一本亚洲免费区，在光學(xué)顯微鏡下图片区激情区小说区另类春色区，三組角膜上皮細(xì)胞均已完全覆蓋激光切削面，新生的角膜上皮細(xì)胞層數(shù)多人人摸人人澡人人，基底層上皮細(xì)胞大小不一欧美丝袜国产。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

實(shí)驗(yàn)組經(jīng)器官培養(yǎng)保存4周后以角膜測(cè)厚儀測(cè)量角膜厚度，然后每個(gè)角膜被分成兩半九九成色视频在线观看，共48個(gè)半片角膜欧美一级一区二区网址，再分成4組，每組12個(gè)半片黄页视频免费在线看。12個(gè)半片用茜素紅-臺(tái)盼藍(lán)染色染色行角膜內(nèi)皮細(xì)胞計(jì)數(shù)亚洲日屄视频在线观看；12個(gè)半片角膜用4%中性福爾馬林溶液固定行HE染色、應(yīng)用免疫組化染色檢測(cè)AQP-1在角膜基質(zhì)和內(nèi)皮細(xì)胞表達(dá)的改變黄网页在线；12個(gè)半片角膜用Na~+-K~+-ATP酶試劑盒測(cè)量角膜內(nèi)皮細(xì)胞Na~+-K~+-ATP酶活性又粗又长又硬的裸交视频；12個(gè)半片角膜用實(shí)時(shí)熒光定量PCR檢測(cè)AQP-1mRNA表達(dá)改變。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

術(shù)后觀察眼球大體情況、測(cè)量角膜厚度18岁黄网。1周后處死實(shí)驗(yàn)動(dòng)物取角膜欧美性爱视频在线观看精品，用茜素紅-臺(tái)盼藍(lán)染色染色行角膜內(nèi)皮細(xì)胞計(jì)數(shù)；用4%中性福爾馬林溶液固定行HE染色免费国产自在线拍、應(yīng)用免疫組化染色檢測(cè)AQP-1在角膜基質(zhì)和內(nèi)皮細(xì)胞表達(dá)的改變人人摸人人色人人操视播放；用Na~+-K~+-ATP酶試劑盒測(cè)量角膜內(nèi)皮細(xì)胞Na~+-K~+-ATP酶活性；實(shí)時(shí)熒光定量PCR檢測(cè)AQP-1mRNA在角膜內(nèi)皮細(xì)胞表達(dá)的改變免费又大又粗又爽的视频；并于正常對(duì)照組角膜比較91精品福利区在线观看。

Corneas and subbasal nerve fibers were examined by confocal microscopy at 1 day, 1 week, 1 month, 3 months and 6 months after the operation.

手術(shù)患者分別在術(shù)后1d、1周黄色视频久久久久、1個(gè)月欧美老人激情综合网欧美综合激情、3個(gè)月、6個(gè)月時(shí)行角膜共焦顯微鏡檢查射一射人人操一操，比較兩組患者術(shù)后角膜上皮下神經(jīng)纖維再生的情況91国内精天天。

The results are unsure for experimental use;The rabbit's corneas that were removed with upper-half of corneal limbal epithelium lamella and erased the center corneal epitheliums were transparent with intact corneal epithelium;In the approach,the corneal and limbal epitheliums were burned with a cotton swab socked in 1 mol/L NaOH,there were 4 rabbits' corneal stroma happened perforation or ulcer and symblepharon,and the other one presented corneal epithelium phenotype.This is an applicable method to create the pathological model of corneal limbal stem cell total deficiency.

結(jié)果表明，處理后4周国产精品国产三级农村A∨，全周角膜緣上皮板層手術(shù)切除igao网为爱搞点激情，中央角膜上皮層用1 mol/L NaOH擦除的5只試驗(yàn)家兔角膜表面全部血管化、結(jié)膜化黄色视频一级链接，未發(fā)生瞼球粘連国产精品免费视频色欲，角膜基質(zhì)膠原纖維完整未見潰瘍、穿孔等病變95av视频，細(xì)胞印跡學(xué)檢查為結(jié)膜表型久久久久精彩视频，可作為實(shí)驗(yàn)性角膜緣干細(xì)胞移植的病理模型；全周角膜緣上皮板層手術(shù)切除乱辈通奷免费视频，中央角膜上皮用生理鹽水擦除的5只試驗(yàn)家兔午夜激情黄网，有2只為結(jié)膜表型，另3只為角膜表型啊进来插视频，觀察期內(nèi)結(jié)果不穩(wěn)定日韩性色自拍视频在线观看网站；半周角膜緣上皮板層手術(shù)切除，中央角膜上皮層用生理鹽水擦除的5只試驗(yàn)家兔中国黄色AV网站，角膜表面透明插入美女嫩穴漫画，全部為角膜表型；直接用1 mol/L NaOH擦除角膜緣和中央角膜上皮的試驗(yàn)家兔操喷视频，有4只角膜基質(zhì)膠原纖維斷裂免费操视频在线、溶解，并伴有嚴(yán)重的潰瘍456亚洲视频在线观看、穿孔免费www你懂的网站视频、瞼球粘連等病變中国野外一级视频片，不能用于移植試驗(yàn)，另1只角膜表面透明自拍欧美日韩制服视频，未見結(jié)膜和新生血管長(zhǎng)入国产一级黄色视频免费看，細(xì)胞印跡學(xué)檢查為角膜表型。

keratoconjunctivitis：角膜結(jié)膜炎

(1)角膜結(jié)膜炎(keratoconjunctivitis)先見眼瞼紅腫,出現(xiàn)小水泡. 涉及角膜時(shí)出現(xiàn)樹狀的潰瘍及耳前淋巴結(jié)腫大. 如病變限于結(jié)膜,大都在2周內(nèi)恢復(fù),但如角膜受損則可影響視力,甚至出現(xiàn)瘢痕,可致失明. (2)外陰陰道炎(vulvovaginitis)外陰部一般紅腫,