Apha amylase was difficult to bind with the substrate, ther fore the hydrolysis rate decreased with time.

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Objective To study the protective effect of coenzyme complex on myocardium under cardiopulmonary bypass in open heart surgery.

目的 觀察輔酶復(fù)合物在體外循環(huán)心內(nèi)直視術(shù)中的心肌保護(hù)作用亚洲精品国产欧美一二。

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

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Chapter 5 covered the quantum chemistry study of the model molecule (410 atoms) of HupA- Acetylcolinesterase complex at HF/4-31G level with point charge model (1929 point charges) to simulate the environment effect on complex active site.

第五章：用HF/4-31G量子化學(xué)方法對單分子狀態(tài)石杉?jí)A甲、活化狀態(tài)的石杉?jí)A甲以及石杉?jí)A甲-乙酰膽堿酯酶復(fù)合物的模型體系（410原子精品日本欧美，1929個(gè)點(diǎn)電荷）進(jìn)行了從頭計(jì)算研究欧美性爱一页，指認(rèn)了對于形成穩(wěn)定復(fù)合物貢獻(xiàn)最大的石杉?jí)A甲分子的基團(tuán)和酶的氨基酸殘基，揭示了活性位點(diǎn)中水分子的重要作用国产www。

According to the experiment results of degradation in field, degradation mechanism of the biodegradable mulch film can include four stages: the first, the material surface appeared deficiency while the surface area and interstice increased of material in the action of natural environment and microorganism; the second, along with the surface area and interstice of material increased and the gap strengthened, the chemical chain of the high polymer's principal chain broke as a result of the hydrolysis or the enzymatic effect in the action of hydration, causing the molecular weight and mechanics function descended; the third, the molecular of high polymer split the low polymer after its physical mechanical function descended, the material decomposed the fragments, the mass defect intensified; the fourth, the low polymer of fragments form in the natural environment, the microorganism, the biological enzyme became the smaller fragments, thus absorbed by the phagocytes, or further turn into CO2 and H2O due to hydrolysis.

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Transition metal complexes based on benimidazoles can be used as simulacrum of biology molecular, even more catalytic activity was found in some of them.

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PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

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The free radical scavenging effects of Meretrix meretrix hydrolysates prepared with papain,neutral protease and acid protease were investigated.

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An enzyme that catalyzes the removal and transfer of hydrogen from a substrate in an oxidation-reduction reaction.

脫氫酶一種酶，能在氧化還原反應(yīng)過程中国产性爱专区，對脫去作用物中的氫并轉(zhuǎn)化氫起催化作用

Methods The DNA chip was fabricated,on which oligonucleotide bougies′length was 19 base pairs,and the concentration was above 15 μmog/L.

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catalase：過氧化氫酶

● 氧化酶(oxidase)氧化酶作用于不同的底物,其共同特征是氧化底物的同時(shí),將氧還原成過氧化氫:● 過氧化氫酶(catalase) 過氧化氫酶是過氧化物酶體的標(biāo)志酶,它的作用是使過氧化氫還原成水: 2H2O2→ O2 + 2H2O.

Influence of substrate concentration on enzymatic action：底物濃度對酶作用的影響

(2)Influence of enzyme concentration on enzymatic action酶濃度對酶... | (3)Influence of substrate concentration on enzymatic action底物濃度對酶作用的影響 | (4)Influence of pH on enzymatic action pH對酶作...

Influence of enzyme concentration on enzymatic action：酶濃度對酶作用的影響

(1)Determination of enzymatic reaction velocity酶反... | (2)Influence of enzyme concentration on enzymatic action酶濃度對酶作用的影響 | (3)Influence of substrate concentration on enzymatic action底物濃...

oxidase：氧化酶

● 氧化酶(oxidase)氧化酶作用于不同的底物,其共同特征是氧化底物的同時(shí),將氧還原成過氧化氫:● 過氧化氫酶(catalase) 過氧化氫酶是過氧化物酶體的標(biāo)志酶,它的作用是使過氧化氫還原成水: 2H2O2→ O2 + 2H2O.

uncompetitive inhibition：反競爭性抑制作用

反競爭性抑制作用(uncompetitive inhibition): 抑制劑只與酶-底物復(fù)合物結(jié)合而不與游離的酶結(jié)合的一種酶促反應(yīng)抑制作用. 這種抑制使Km和υmax都變小但υmax/Km不變. 很大一類復(fù)雜的蛋白質(zhì)物質(zhì) [enzyme;ferment],在促進(jìn)可逆反應(yīng)(如水解和氧化)方面起著像催化劑一樣的作用.

zymoid：類酶的

zymohydrolysis酶解(作用);酶性發(fā)酵 | zymoid類酶的 | zymolite酶解物

zymolytic：酶分解的

zymolyte 酶底物 酶解物 酶作用物 | zymolytic 酶分解的 | zymometer 發(fā)酵計(jì)

zymolytic：酶解(作用)的;酶性發(fā)酵的

zymolyte酶解物 | zymolytic酶解(作用)的;酶性發(fā)酵的 | zymometer發(fā)酵計(jì)

Enzyme substrates：酶作用物(培養(yǎng)基)

Electrophoresis Materials 電泳材料 | Enzyme substrates 酶作用物(培養(yǎng)基) | Fluorescent Labels Indicators 熒光標(biāo)記物、指示劑

transferase：轉(zhuǎn)移酶

再有就是依據(jù)催化的反應(yīng)性質(zhì)命名,如催化氧化還原反應(yīng)的脫氫酶(dehydrogenase)绿帽人妻91社区、基團(tuán)轉(zhuǎn)移的轉(zhuǎn)移酶(transferase)等. 為了更進(jìn)一步區(qū)分,可加上反應(yīng)的作用物名稱,如乳酸脫氫酶国产拍在线视频、琥珀酸脫氫酶(succinate dehydrogenase)、谷丙轉(zhuǎn)氨酶(glutamic-pyruvic transaminase),