OBJECTIVE : to analyse the replication activity of NS5B proteins of HCV genotype 1a and 1b when use 3'terminuses of negative RNA strand from HCV 1a, 1b and 2a as templates by performing RdRp assay in vitro and offer some useful data to optimize the model for the screening of anti-HCV drugs targeting at NS5B.

目的：通過(guò)體外的RNA依賴的RNA聚合反應(yīng)色中色污网站，分別比較HCV 1a和1b型來(lái)源的NS5B蛋白以不同HCV基因型負(fù)鏈3'末端RNA為模板時(shí)的復(fù)制活性色欲aⅴ4无码，為優(yōu)化以NS5B為靶點(diǎn)的抗HCV藥物篩選模型提供理論依據(jù)九九影院精品。

The 3'terminuses of negative RNA strand from 1a, 1b and 2a were prepared via in vitro transcription, using these RNA as temlates and the two purified NS5B proteins as polymerase respectively we conducted the RdRp assay in vitro.

分別以體外轉(zhuǎn)錄的HCV 1a久草视频免费在线、1b和2a型負(fù)鏈3'末端RNA作為模板RNA，NS5B1和NS5B2為聚合酶進(jìn)行體外RdRp反應(yīng)久久毛片免费全部播放无码，Northern blot檢測(cè)產(chǎn)物合成情況中国内地最新一级A毛片免费不卡视频。

The purified proteins had RdRp activity, and could synthesize RNA products similar in length to the RNA templates using the 3'terminuses of HCV genotype 1a, 1b and 2a negative RNA strand.

純化的NS5B蛋白具有體外RdRp活性，能夠以HCV負(fù)鏈3'末端RNA為模板體外合成一條與模板大小相近的產(chǎn)物亚洲A∨无码乱码在线观着性色。

Results Positive HCV RNA was found in supernatants of B cell line. HCV Ag and HCV RNA were also showed positive. Electron microscopy observed HCV spherical virus like particles with a diameter of approximately 65 nm and 110nm and the "bud mutation"of HCV in the cytoplasmic vesicles of B lymphocytes.

結(jié)果細(xì)胞系上清液中HCV RNA呈陽(yáng)性视色网、视色视频，細(xì)胞內(nèi)HCV抗原及HCV RNA均呈陽(yáng)性，電鏡觀察結(jié)果發(fā)現(xiàn)細(xì)胞內(nèi)存在65nm和110nm圓球型病毒顆粒，并可見(jiàn)病毒芽生形成現(xiàn)象国产精品一区二区视频。

RNA interferencewas the process by which agene was suppressed using double-stranded RNA.The phenomenon has been observed as gene expression mediated mechanism in protozoan,elegans,fungi,insect,and mammal etc.,which was very old and highly conserved in evlution.

RNA干擾(RNAi视频在线精品，RNA interference)是dsRNA介導(dǎo)的特異性基因表達(dá)安靜表象，是生物界一種陳舊而且進(jìn)化上高度保守的基因表達(dá)調(diào)節(jié)機(jī)制免费看黄片链接。

It contains 215 bp, named as ZM908. Biosoftware anlysis sug gests that ZM908 gene properly belongs to the mRNA-like non-coding RNA gene which is purposed to lack of obvious open reading frame (the longest possibly contains 98 AA), contain poly tale and have stable RNA structure in vivo. ZM908 contains high conserved nucleotide sequence with ZM401 (including 371-598bp, 698-847bp and 929-1047bp in ZM908). The highest conserved sequence reaches 86%.The RT-PCR analysis result revealed that ZM908 is transcriptable.

基因分析顯示這段序列含1215bp超粉嫩000福利视频合集，命名為ZM908；采用生物學(xué)軟件對(duì)ZM908序列和結(jié)構(gòu)進(jìn)行分析日韩偷窥自拍系列视频在线播放网站，表明該基因缺乏明顯的開(kāi)放閱讀框架欧美视频在线观看图片小说，序列中最長(zhǎng)可能的開(kāi)放閱讀框架僅編碼98個(gè)氨基酸，具有poly尾部結(jié)構(gòu)嫩草爱爱视频，RNA分子具有穩(wěn)定的二級(jí)結(jié)構(gòu)，符合非編碼RNA基因的特點(diǎn)黄色AV免费网址，可能屬于類似于mRNA的非編碼基因91P0RNY大屁股人妻；ZM908與ZM401有高度保守的區(qū)段(ZM908序列中有三段和ZM401是高度保守的，分別為371-598bp美妇福利视频，698-847bp和929-1047bp)日韩精品无码9p，相似性最高達(dá)到86%。

Sigma factor is a component of the bacterial RNA polymerase holoenzyme which is composed of RNA core enzyme and Sigma factor.

因子是RNA聚合酶全酶的一個(gè)組成成分日韩无码啪啪片，RNA聚合酶全酶是由核心酶和σ因子組成影音先锋AV资源网无码,国产v。

The results presented in this paper indicated that sodium salt of 3,7-bishemis uccinate of 7-hydroxycholesterol (RS 634) at the dose of 45mg/kg/day (×7,ip) could increase DNA, RNA and protein syntheses in bone marrow cells and protein synthesis in liver,spleen and serum, but no markedly effects on DNA and RNA synthesis in liver and spleen of mice bearing EAC were observed, In the subacute test on rats RS 034 (400mg/kg/day, x30 ip) showed no evident effects on the number of RBC, WBC and the percentage of lym...

本文報(bào)告的結(jié)果表明7β-羥基膽固醇雙琥珀酸單酯鈉(RS 024)在有效抗腫瘤劑量下(45mg/kg/day，×7ip)對(duì)荷瘤小鼠骨髓細(xì)胞DNA久久精品微拍国产微、RNA和蛋白質(zhì)合成有促進(jìn)作用99久久久久，對(duì)肝、脾的DNA美女黄片毛片在线看、RNA合成無(wú)顯著影響日韩精品无码一区二区三区99，但能促進(jìn)肝，脾中及血清蛋白質(zhì)合成日黄特一级黄色A片。按400mg/kg/day連續(xù)給藥30天色在线观看黄视频，對(duì)大鼠紅細(xì)胞、白細(xì)胞數(shù)及淋巴細(xì)胞百分率無(wú)明顯影響国产黄A一级毛片视频。提示該藥在有效抗癌劑量下毒性較小一级一级一级一级毛片，并在一定程度上對(duì)免疫功能有增強(qiáng)作用。

Using nanogram quantities on total RNA, the usage of dIVT protocol preserved about 70% information of gene expression when compared with those detected by the one-cycle T7 RNA linear amplification.

從納克量級(jí)的總RNA起始在线免费看一级片，本方法和常規(guī)的RNA單輪線性擴(kuò)增法相比国产三级片黑免费，仍然準(zhǔn)確地保留了約70%的基因表達(dá)信息。

However, some transposon classes were apparently targeted by only one of these RNA classes, which suggested that piRNAs or siRNAs preferentially control certain TEs.

然而欧美熟妇丰满肥白大屁股，一些轉(zhuǎn)座子明顯的僅僅被一種RNA所鎖定亚洲全黄无码一级，這意味著pi RNA或si RNA優(yōu)先調(diào)控特定的TEs。