For cloning antifreeze protein gene from a desert darkling beetle Microdera punctipenis dzunarica in Xinjiang, the primers were designed according to the core sequence of AFP gene deposited in GenBank, and the cDNA fragment about 294 bp named as MpAFP5 was amplified with the RT-PCR and 3'-RACE technique. Sequence analysis revealed that the cloned cDNA fragment named as MpAFP5 coded the mature peptide of AFP.

根據(jù)GenBank中已發(fā)表的昆蟲抗凍蛋白基因的保守序列設(shè)計引物2018年一级黄片，利用RT-PCR技術(shù)結(jié)合3'-RACE擴增的方法，從新疆荒漠昆蟲準(zhǔn)噶爾小胸鱉甲Microdera punctipenis dzunarica中獲得了長約294 bp不含信號肽的抗凍蛋白cDNA片段，命名為MpAFP5欧美一级做一级a做片性，其全長序列為363 bp(GenBank注冊號為：AY821792)啊用力插视频。

Methods: RT - PCR and immunoblot were used to analyse DCC gene expression on 36 cases of large intestinal Cancer.

采用RT-PCR和免疫印跡法檢測分析了36例大腸癌抑癌基因DCC在mRNA和蛋白水平的表達(dá)。

METHODS:To detect the expressions of CD44v mRNA and DCC mRNA in PLC by RT-PCR.

應(yīng)用RT-PCR檢測30例原發(fā)性肝癌中CD44v mRNA和DCC mRNA的表達(dá)情況成年美女黄网站18禁免费动态图，結(jié)合其轉(zhuǎn)移相關(guān)的臨床病理指標(biāo)分析它們間的關(guān)系以及在轉(zhuǎn)移中的作用国产毛片在线看线。

Methods: Expression levels of MMP10 gene and protein in human spontaneous abortion decidua were determined by RT-PCR, Western blotting and distribution by immunohistochemistry.

利用RT-PCR和Western blotting技術(shù)分別檢測MMP10基因和蛋白在人正常蛻膜和自然流產(chǎn)蛻膜的表達(dá)水平、利用免疫組織化學(xué)技術(shù)檢測MMP10在人正常蛻膜和自然流產(chǎn)蛻膜組織中的分布久久久久国产精品爆乳。

The liver fibrotic model was induced by CCl4 i.p for 5wk. Telmisartan was given at the same time model establishment in each intervened group by administration lasted for 6wk. Decollate to get the blood of all rats after 7 weeks . ALT色一区二区、AST and ALB of serum was detected by automatic biochemistry equipment. Hyaluronic acid was detected by ELISA detection. Liver and spleen was obtained to calculate the index. The liver pathological changes were observed in each group of rats after HE staining to evaluate the therapeutic effect of Telmisartan; TGF-β1、PPAR-γand PDGF of liver tissue was detected by the method of immunohistochemistry respectively; The mRNA expression of TGF-β1亚洲高偷拍一区二区三区、MMP-1好大好爽插我视频在线播放免费、TIMP-1、α-SMA全球二级三级黄视频、PPAR-γand iNOS was detected by RT-PCR detection, respectively.

腹腔注射CCl4制備SD大鼠肝纖維化動物模型动漫在线，連續(xù)灌胃6周，處死動物取血就是操就是干，檢測血清ALT人人操人人看人人插人人干、AST、ALB国产各种盗摄视频；ELISA法測定血清透明質(zhì)酸一级一级女人18毛片；取肝、睥日免费在线精品，并計算肝性无码高潮在线网站、脾指數(shù)，組織切片HE染色后進行組織病理學(xué)療效評估怕怕怕免费网站18以上看；用SP免疫組化法測定肝組織TGF-β1探花自拍日韩视频在线、PPAR-γ及PDGF蛋白表達(dá)；半定量RT-PCR方法檢測肝組織TGF-β1色色色789、MMP-1初黄色视频在线免费观看、TIMP-1、α-SMA一个色A片网、、PPAR-γ和iNOSmRNA的表達(dá)日韩欧美到亚洲。

Hepcidin mRNA was expressed in the mice brain.Total RNA was extracted from choroid plexus, cerebral cortex, diencephalon and mesencephalon, cerebellum, medulla oblongata and pons in mice. RT-PCR analysis demonstrated that hepcidin mRNA was expressed in all areas of mouse brain, in choroid plexus the mRNA expression was highest, although its level is generally lower than that of liver. The PCR products were purified and verified the sequence.

小鼠腦內(nèi)有hepcidin基因表達(dá)提取小鼠腦脈絡(luò)叢、大腦皮層开心五月丁香激情av综合一区二区三区、間腦及中腦免费网站日本A∨、小腦和腦橋與延髓組織總RNA進行RT-PCR表明，大腦皮層国产av网站飞、間腦和中腦日韩写真福利资源网站、小腦、腦橋與延髓、脈絡(luò)叢均有hepcidin表達(dá)你懂的免费在线视频，其中以脈絡(luò)叢表達(dá)較高欧美性爱爱欧美，但與肝臟相比，表達(dá)量較低91AⅤ美国黄片。

The best optimum conditions of the Agrobacterium mediated genetic transfornation were that leaves explants were immersed in the diluter 10 times Agrobacterium bacilli at OD600 of 0.6 for 8 minutes after being pre-cultured for 2 days,and then co-cultivated for 3 days.Then these explants were transferred to selected and rooting medium which contained 50 mg/L Kanamycin and 500 mg/L Carbenicillin.According to the transformation system, 54 transgenic plants were initially screened,then after PCR analysis and RT-PCR analysis 25 transgenic plants were obtained.

根癌農(nóng)桿菌介導(dǎo)的芪合酶基因遺傳轉(zhuǎn)化的最佳條件為：將預(yù)培養(yǎng)2 d的煙草外植體3D动漫爆操插，用稀釋10倍的GV3101菌株菌液（OD600=0.6）浸染8 min后，共培養(yǎng)3 d@免费毛片网\，然后轉(zhuǎn)入含卡那霉素50 mg/L和羧芐青霉素500 mg/L的篩選分化培養(yǎng)基上誘導(dǎo)分化久操免费精品视频，待抗性芽長到2～3 cm后，轉(zhuǎn)入含Km 50 mg/L和Cb 500 mg/L的篩選生根培養(yǎng)基上進行篩選国产粗大猛烈进出高潮视频大全，在以上最佳遺傳轉(zhuǎn)化條件下精品乱伦视频免费播放，初步篩選到54株轉(zhuǎn)化體，經(jīng)PCR和RT-PCR檢測獲得25株轉(zhuǎn)化煙草植株国产激情刺激精品狼区。

It was a new mousegene in GenBank and named as SRG-L(spermatogenesis related gene expressed in the latestages of spermatogenic cells). The sequence of this cDNA has been deposited in GenBankwith the Accession No. AY352586. 2. Analyses of expression characteristics of SRG-L during mouse spermatogenesis.The results of RT-PCR and Northern Blotting indicated that no expression signal of thisgene could be observed in primitive type A spermatogonia and type B spermatogonia. Asignal first appeared in diplotene/pachytene spermatocytes, and was significantlyintensified in round spermatids and elongating spermatids, findly went down to beundetectable in mature spermatozoa.

RT-PCR和Northern Blotting分析表明：該基因在早期A型和B型精原細(xì)胞中不表達(dá)亚洲福利片网站，從雙線期初級精母細(xì)胞階段開始表達(dá)并逐漸上調(diào)，在粗線期初級精母細(xì)胞免费不卡在线A片、圓形精子細(xì)胞及長形精子細(xì)胞中均檢測到高水平的mRNA黄网站在看视频在线，但在成熟精子中消失，具有明顯的階段特異性表達(dá)特征人人人人人人人看，并且組織特異性表達(dá)分析顯示該基因在睪丸組織中的呈高表達(dá)国产精品视频7171。

According to the gallus colony stimulating factor(colony-stimulating factor,CSF)cDNA sequences published by GenBank,a pairs of specific primer was designed,the local dorking was injected 100 μg/mL ConA,total RNA was extracted from feeding 24 hours lymphocyte from spleen,then dorking colony stimulating factorcDNA fragment was cloned by RT-PCR technology.

根據(jù)GenBank中報道的紅原錦雞集落刺激因子(Colony-stimulatingfactor，CSF)cDNA序列AAA黄色视频免费观看，利用Primer Premier5.0軟件設(shè)計一對特異性引物国产精品视频馒头一线天，對本地肉雞注射100μg/mL ConA試劑，飼養(yǎng)24 h后尹人综合日本在线www官网，取脾臟提取淋巴細(xì)胞總RNA十八禁欧美日韩，利用RT-PCR技術(shù)克隆集落刺激因子的cDNA片段。

According to the gallus colony stimulating factor (colony-stimulating factor, CSF) cDNA sequences published by GenBank, a pairs of specific primer was designed, the local dorking was injected 100 μg/mL ConA, total RNA was extracted from feeding 24 hours lymphocyte from spleen, then dorking colony stimulating factor cDNA fragment was cloned by RT-PCR technology.

根據(jù)GenBank中報道的紅原錦雞集落刺激因子(Colony-stimulatingfactor最新福利网址在线， CSF)cDNA序列人人操,人人摸97，利用Primer Premier5.0軟件設(shè)計一對特異性引物，對本地肉雞注射100μg/mL ConA試劑日韩欧美黄色网站，飼養(yǎng)24 h后三级片毛片免费看，取脾臟提取淋巴細(xì)胞總RNA，利用RT-PCR技術(shù)克隆集落刺激因子的cDNA片段欧美18XXOO视频在线群。