Two 15nt complementary ssDNA primers were ligated into double stranded satRNA.Complete sequence of satRNA was obtained by RT-PCR using these 15nt ssDNA as amplification primers.Phylogenetic analysis showed that the identity of this 383nt satellite and some documented satRNAs was 72.6 to 99.5% at the nucleotide level.

以常規(guī)引物對感病植物的總RNA進行RT-PCR擴增，獲得CMV RNA3全長克隆精品天堂很干视频，經(jīng)測序顯示該RNA3序列屬于CMV亞組Ⅱ；通過在衛(wèi)星dsRNA兩端分別加上15nt的單鏈DNA接頭，以接頭互補序列為引物進行擴增獲得一個383nt的衛(wèi)星RNA的全長序列人妻啪啪色视频。

Finally a all-digital recorder system based on RAID5 which has a RT-Linux real-time software operation system and intermediate/video frequency acquisition card driver to overcome such problems as real-time quality reliability chunnel expansibility and standardization.

最終提出了以RT-Linux實時操作系統(tǒng)為軟件平臺和專門設計的中頻和視頻采集卡驅(qū)動為底層硬件平臺的基于RAID5陣列的全數(shù)字記錄系統(tǒng)，全面解決了記錄實時性日韩乱码一区二区三区、可靠性以及通道擴展性和標準化的技術(shù)難題黄色一级A片在线播放视频在线观看免费。

Based on the theory of large eddy simulation and the method of VOF , the initial breakup process of fuel liquid jet, which eject from the nozzle exit at the time scale of micro-second level, is investigated. The mechanism of primary atomization is analyzed, and the initial breakup model of liquid jet is presented. By modeling and numerical simulation of atomization for a diesel nozzle under the given conditions, the initial umbrella-like spray shapes are obtained, which fits better with the experimental results. Meanwhile, the droplets secondary breakup process, such as leeward RT breakup, dumbbell-like breakup and droplets coalescence, etc. are exhibited visually.

基于大渦模擬LES理論和VOF方法亚州黄色网站中文字幕，考察了燃油噴霧初始時刻即時間尺度為微秒級的液柱破碎過程，分析了初始破碎的機理无码专区无码专区，給出了該時間尺度下液柱初始破碎過程的模型800凹凸视频在线观看网址；通過對一特定條件下的柴油機噴油器的建模和噴霧過程的大渦數(shù)值模擬，獲得了液柱初始時刻"傘狀頭部"的噴霧形態(tài)可以在线看av的黄色网站，所得計算結(jié)果與相應的試驗數(shù)據(jù)符合較好69人人操人人爱；數(shù)值模擬還直觀地展現(xiàn)了液滴背風RT破碎、啞鈴型破碎以及液滴的聚集融合等液滴的二次霧化過程国产精品免费福利久久。

Methods The designed flow chamber had provided flow shear stress at 0.5, 1 Pa (5 and 10 dyn/cm^2) for a period of 1 h, 3 hand 6 h respectively. Semi-quantitive RT-PCR was used to detect the expression of megalin/cubilin mRNA in cultured renal tubular epithelial cells.

應用流室系統(tǒng)提供0.5人妻丝袜中文字幕三区日韩、1Pa(5、10 dyn/平方公分)的剪切應力處理培養(yǎng)的腎小管上皮細胞黄色的视频国产在线观看，分別作用1午夜麻豆国产精品视频、3、6h后欧美精品热视频在线观看，用RT-PCR法檢測megalin/cubilin mRNA的表達黄片A片视频免费在线观看。

The protein and mRNA level of MCP-1 and IL-8 in U937 cells were determined by RT-PCR and ELISA.

用ELISA和RT-PCR方法測定MCP-1和IL-8的蛋白和mRNA水平。

Results: A clear-cut endocardium image was obtained by both RT-3DE and CMRI in all but 1 volunteer.

RT-3DE是一種定量評價左心室容量-時間曲線的可行人看人爽人操人摸人日人碰、準確的新方法最新黄色视频在线播放。

It was proved by RT-PCR that Pinb gene expressed itself in grain endosperm.

RT-PCR證實了Pinb基因在籽粒胚乳中的表達。

Expressions of eNOS and COX2 mRNA and proteins were assessed by immunohistochemical technique and RT-PCR.

用免疫組化和RT-PCR技術(shù)檢測肺組織eNOS和COX2 mRNA和蛋白表達欧美精品A∨欧洲精品。

Methods The cDNA coding sequencing of enterokinase light chain was amplified by RT-PCR from mouse dodecadactylon mucosa and cloned into pET32a expression plasmid. The recombinant enterokinase light chain was expressed in BL21(DE3) and purified with Ni-affinity chromatography.

采用RT-PCR從C57BL/6J小鼠的十二指腸腸系膜黏膜組織中釣取腸激酶輕鏈的cDNA妇女人人操人人干，將其克隆入pET32a原核表達載體中，并在大腸桿菌BL21(DE3)中進行表達色噜噜午夜午伦视频一区二区，然后以鎳親和層析法對表達產(chǎn)物進行純化欧美成性互相。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

為克隆表達牛腸激酶輕鏈編碼基因，以期應用于融合蛋白的切割與純化一级做性色a爰片久久毛片体验区，從市售北方黃牛十二指腸組織中提取總RNA色色网五月，以RT-PCR方法擴增其cDNA片段，將此片段克隆于pUCmT載體中av体验区，經(jīng)過特異性限制性內(nèi)切酶酶切分析片段正確后性爱不卡视频网站，進行全序列分析。