Methods SLN of 40 patients with breast cancer were performed histological analysis by hematoxylin and eosin staining, and the expression level of CK 19 in these nodes were determined by quantitative RT-PCR.

目的 探討定量RT-PCR法檢測細胞角蛋白(CK19)在乳腺癌前哨淋巴結中的表達欧美人人做人人爱，提高前哨淋巴結活檢中微轉移的檢出率。

In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期亚洲天堂国产制服，高度表達的合子轉錄本仍在所有的胚盤細胞中，但至90%下包期時老乱女中文字幕熟女熟妇，pou2的表達向胚胎背部的正中線匯聚国亚洲视频，集中在神經(jīng)板的兩側區(qū)域和腦部的兩條橫向條帶在100%下包期時，pou2的表達集中在神經(jīng)板的中間區(qū)域以及預期形成的中后腦區(qū)域至體節(jié)期時免费激情网址，轉錄本消失五月天亚洲综合在线电影，這與RT-PCR結果高度一致。

Preadipocytes from epiploon of newborn calf were cultured by monolayer adherent method, and total RNA of the preadipocytes was extracted on day 4,6,8,10,12,14 and 16 post-culture, respec-tively. The abundance of ADPN mRNA and HSL mRNA was detected by real-time fluorescent quanti-tative RT-PCR during differentiation period from preadipocyte to adipocyte.

選取犢牛大網(wǎng)膜脂肪組織中国精品Jizz免费看，進行牛前脂肪細胞的單層貼壁培養(yǎng)精品欧美视频在线观看视频，在細胞接種后第4、6欧美A以一区二区三区、8黄色在线看片黄色在线看片、10、1218禁黄网站无码无遮挡免费、14亚洲欧洲春色另类、16d分別提取細胞總RNA，采用實時熒光定量RT-PCR方法欧美18禁止看网站，檢測了體外培養(yǎng)的犢牛前脂肪細胞不同時期ADPN基因mRNA和HSL基因mRNA的表達情況麻豆久久。

Methods: Three aspects were studied in the epithelization of skin of PAI-3 with the techniques of Immunohistochemistry,Immunocytochemistry,RT-PCR and the analysis of PAI-3 in cornified envelope.

采用免疫組織化學(Immunohistochemistry，IHC)精品一级A片中文字幕、(Immunocytochemistry欧美XXXX做受欧美18，ICC)和逆轉錄-聚合酶鏈反應(reverse transcription PCR，RT-PCR )技術及KC角質殼的提取A级毛片全部免费高清视频人人，從三個方面研究了PAI-3 在人皮膚上皮化中的表達及作用Siwaav.com。

Therefore the established one-step RT-PCR technique provided a sensitive, specific and reliable method for diagnosis and epizootic study of the BVDV.

初步結果表明所建立的一步法RT-PCR技術可用于牛病毒性腹瀉／粘膜病的檢測及流行病學調(diào)查。

Key words: avian influenza virus; one-step RT-PCR; diagnoses expeditiousness

禽流感病毒口爆在线；一步法RT-PCR国产精品自拍网；快速診斷

Primary atrial tumor has obvious characteristic on RT-3DE: All the atrial tumor display the same size of stereoimages as the tumor themselves. There is a clear boundary between the stereoimage of the myxoma and the atrial wall and there exists a short pedicle connecting the atrial myxoma to the atrial wall or the interatrial septum. The body of the atrial myxoma moves to and fro through the atrium and the atrioventricular valve with the contraction and relaxation of the heart with a fixative pedicle. Atrial myxoma leads to relative atrioventricular valve incompetence during systolic phase and relative atrioventricular valve stenosis during diastolic phase, but doesn't result in pathological changes of atrioventricular valve which restore to normal after exsection of the atrial myxoma. Malignant atrial tumor closely and extensively contact with atrial wall without obvious boundary, pedicle and movement with the contraction and relaxation of the heart.

所有心房腫瘤RT-3DE均實時顯示與腫瘤大小相同的立體形態(tài)圖像；心房黏液瘤立體形態(tài)圖像與心房壁界限明顯一级一级一片在线观看，都通過一短蒂與房間隔或心房壁相連婷婷色五月开心综合先锋影音，瘤體以蒂為固定點隨心臟收縮www777miqi、舒張在心房與房室瓣口之間往返運動；心房黏液瘤收縮期瘤體導致房室瓣相對關閉不全123区无码乱伦，舒張期瘤體導致房室瓣相對狹窄亚洲不卡无码精品一区二区，但瘤體不引起房室瓣器質性病變，瘤體摘除后房室瓣功能即恢復正常男女八x八x视频网站；惡性心房肺瘤立體形態(tài)圖像與心房壁關系密切国产999国内精品视频免费，界限不明顯，接觸范圍廣泛182tv免费精品视频，無蒂亚洲国产色图视频，瘤體不隨心臟收縮、舒張運動亚洲动漫第18页。

The fragment of amplification was 247bp in size using the external primers.After optimization of the ratio of the internal to the external primers,concentration of dNTP and Mg2 +,temperature and time of reaction,a RT-LAMP method for specific detection of CDV was established successfully.

通過對反應條件中內(nèi)www.九九久久、外引物的比例、dNTP濃度亚洲国产综合猎奇自在线、Mg2+濃度亚洲综合一区二区精品久久、反應溫度和時間等條件逐一優(yōu)化，成功建立了犬瘟熱病毒的RT-LAMP檢測方法欧美色图一区。

According to experimental results and etiopathogenisis and pathology of osteoporosis , and analyzing review investigation on hepcidin四虎成人入口在线观看视频网站免费、associations of bone metabolism and iron metabolism and relations of ferri ion and calcium ion,we can make conclusions as follows: 1、We applied single manmade intervention approach to induce osteoporosis model in this study, there was no other influential factor. In the termination of test , rats bone density and bone histological anatomy indicated that osteoporosis model was creditable,meanwhile hepatic functional enzyme and hepatic microtome section demonstrated that hepatic function had noci-influence induced by medicinal herbs resource. Accordingly, we could believe that hepatic hepcidin gene expression and serum hepcidin contents can represent internal iron metabolism changes at selected test time. 2日韩亚洲国产黑人色、In this investigation, different findings between test group and control group indicated that:according to changes of hepcidin gene expression measured by RT-PCR and changes of serum hepcidin contents determined by ELISA kit , there were correlations between hepcidin and rat osteoporosis model induced by retinoic acid. 3欧美黄色网站有哪些、Under the condition of this investigation,there was a interactive hypothesis as follows: Hepcidin iron metabolism←→ferriion←→calcium ion←→osteoporosis.

根據(jù)本次實驗結果，對骨質疏松的病因病理的認識及鐵代謝與鐵調(diào)素中文字幕视频精品免费黑人、骨代謝與鐵代謝關系日韩快播自拍图片在线观看、鐵離子與鈣離子的關系系列研究的回顧，經(jīng)分析得出如下結論：1一级毛片高清网站、本研究骨質疏松模型采用單一人工干預方法96人人操，沒有其他影響因素；實驗結束時大鼠骨密度精品人妻中文字幕1区、骨組織學檢查表明骨質疏松形成免费av在线不卡澳门，同時肝臟功能酶和HE切片顯示肝臟功能未受藥源性產(chǎn)生明顯損傷性影響；因此欧美性网，同期不同時間組大鼠肝臟鐵調(diào)素基因表達红豆视频直播免费观看在线、血清鐵調(diào)素含量可以代表體內(nèi)鐵代謝不同時間的變化。2欧美性爱视频在线观看视频、在本研究中国产黄色视频免费看日韩黄色视频免费看，模型組與對照組研究結果的差異表明：鐵調(diào)素基因RT-PCR變化、血清鐵調(diào)素ELISA測定含量變化與維甲酸制作的大鼠骨質疏松模型形成有實驗相關性国产毛片黄片。3www.高清色色视频.cnm、在本研究平臺下色网视频A，形成了一個互相影響的假設圖：鐵調(diào)素←→鐵代謝←→鐵離子←→鈣離子骨質疏松。

Methods The expression of fez gene was detected by RT-PCR in thirty-six cases of esophageal cancer tissues, matched paracancer tissues and tissues far away from tumor.

采用RT-PCR檢測36例食管鱗狀細胞癌癌組織717午夜理论片在线观看影片、相應的癌旁組織和遠癌組織中fez mRNA的表達情況欧美日韩中国产视频。