In this study, semi-quantitative RT-PCR, ABC immunohistochemistry assay, flow cytometry analysis and luciferase/luciferin assay were used to study the expression and function of CD39 in seven hematopoietic cell lines.

采用半定量RT-PCR、ABC免疫酶標(biāo)和流式細(xì)胞術(shù)以及熒光素/熒光素酶法国产韩国精品福利小视频，研究了七個血源細(xì)胞系中CD39的表達(dá)和功能一级黄片91。

In order to detect the expression of N5, N10-methylene-tetra hydrofolate reductase gene in Epstein-Barr virus transformed human B lymphoblast, as well as the cDNA sequence, human peripheral blood was transformed by EBV to build B lymphoblast cell line. Total RNA was isolated from the cell culture. Variant segments of MTHFR cDNA were amplified using RT－PCR protocol and analysed by polyacrylamide gel electrophoresis.

為檢測用EB病毒轉(zhuǎn)化建立的永生性人B淋巴母細(xì)胞株中N5，N10－亞甲基四氫葉酸還原酶基因的表達(dá)及其cDNA序列亚洲人妻不卡无码视频，用EB病毒轉(zhuǎn)化人外周血B淋巴細(xì)胞亚洲最大无码A∨在线观看，建立永生性細(xì)胞株后人人妻人人艹，從培養(yǎng)細(xì)胞中提取總RNA，用RT-PCR法擴增MTHFR基因cDNA的不同片段网友自拍日韩经典视频，進(jìn)行PAGE電泳分析和cDNA序列測定欧美午夜三级片。

Methods OS732 was treated with 10^(-5)MATRA for 6 days, then we observed cell morphology, counted cell number, analyzed cell cycles by flow cytometry and detected mRNA expressions of cyclinD (Dl, D2, D3), CDK4, P21 by semi-quantity RT-PCR. Treatmed with 10^(-5)MATRA, The nucleus of OS732 cells became small and condensed, and cytoplasms expanded.

用10^(-5)MATRA作用骨肉瘤細(xì)胞OS732， 6d后www.99riav22，進(jìn)行細(xì)胞形態(tài)學(xué)觀察亚州黄色专区毛毛片、活細(xì)胞計數(shù)、流式細(xì)胞儀分析細(xì)胞周期婷婷五月丁香在线、半定量RT-PCR檢測細(xì)胞周期調(diào)控蛋白CyclinD(D1国产在线观看www、D2、D3)亚洲欧美色图13p、CDK4亚洲91在线综合、P21 mRNA表達(dá)的改變。

Methods The level of EGF mRNA in the cortex and medulla of porcine kidney was measured by RT-PCR technique.

采用RT-PCR技術(shù)分別檢測豬腎皮質(zhì)和髓質(zhì)中的EGF mRNA活性欧美日韩国产综合一。

By immunohistochemistry and semiquantitative RT-PCR analysis, we were the first to demonstrate that AdM mRNA and immunoreactive AdM exists in the rat brain distributed in cerebral cortex, paraventricular tissues, hypothalamus, mesencephalon, medulla oblongata and cerebellum, and AdM mRNA was also found in the human brain.

應(yīng)用免疫組織化學(xué)和半定量RT-PCR方法99久久精品国产一区二区蜜芽，首次發(fā)現(xiàn)在大鼠的腦內(nèi)国产A∨爱情动作片免费观看，包括大腦皮層三级性视频、室旁組織、下丘腦久久久久精品久久久久免费、中腦十八女免费毛片、延髓和小腦均有AdM mRNA及AdM的免疫活性物質(zhì)存在；在人腦內(nèi)也含有AdM mRNA人人干97。

Methods: Twenty-four Kkay mice were divided into three groups(n=8):diabetes group,methionine-diet group,folic acid and vitamin B12 treatment group. C57Bl/6 mice were used as controls(C57). Four months later,the mice were sacrificed and their serum levels of Hcy,folic acid and vitamin B12 were measured by fluorescence polarization immunoassay. Pathological changes in kidneys were examined with the periodic acid schiff method with periodic acid-silver methenamine staining under electron microscope. Expression of MMP-9 protein or mRNA was detected by RT-PCR with immunohistochemical staining. Results: Different morphological changes of DN were found in Kkay mice.

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The method on trans fatty acids analysis in food is very important. The effects of methylate sodium, potassium hydroxide, sulfuric acid and boron trifluoride in methanol and their different concentration and dosage on TFA analysis were studied. The chromatographic condition and the influence of time and temperature on the esterifation of TFA using boron trifluoride in methanol were optimized. A method for analyzing TFA were established by GC based on boron trifluoride in methanol and RT-2560(100m×0.25mm×0.2μm) capillary column.

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RT-PCR was applied to detect changes of hepatic hepcidin gene expression on model different stage. Bone density was detected and decalcification bone microtome section and hepatic histological section were observed and analyzed on the 14th day. Results Compared with control group, hepatic hepcidin gene expression of test group on different stage had obvious differences.

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The results showed that the virus isolation and identification could generate considerably reliable results but the procedure was complicated and costed long time; microtomy fluorescent antibody detection was fast, but the specificity was low and needed to exclude the false positive from the results. Sandwich ELISA detection combined the merits of the former two methods, but it generated the false negative results when the samples were in bad preservation. Compared with the others, RT-PCR was fast, high sensitive and especially useful for detecting the CSFV directly from the samples. The specificity could be raised by using the nested-PCR to detect the CSFV from the simples, especially when the simples were in bad preservation.

結(jié)果顯示十八以下勿进色禁网站永久，病毒分離鑒定法準(zhǔn)確性較高，診斷比較容易日韩一级电影WWW.COM，但存在試驗步驟繁瑣精品大片在线免费，所需時間長的缺點；熒光抗體檢測法所需時間較短国产在线黄色，但需要經(jīng)驗比較豐富人員來判定結(jié)果在线观看AV资源站捆绑，且存在假陽性結(jié)果，特異性比較差一个人看的免费无码视频网站；RT-PCR檢測法具有快速性生活黄色网站、敏感等優(yōu)點，特別是樣品保存不理想時更有價值曰本无码一区，利用套式PCR可以提高檢測的特異性日本,三级黄色片；夾心ELISA檢測法具有快速、敏感等優(yōu)點自拍黄色小视频，但在樣品保存不理想時會出現(xiàn)假陰性結(jié)果真实国产3p视频。

Methods RT-PCR and RACE methods were applied to clone the full-length panallergen gene from Caryota mitis pollen.

方法利用RT-PCR結(jié)合RACE技術(shù)克隆短穗魚尾葵花粉中泛變應(yīng)原profilin的全長基因碰人人摸人人爽，并進(jìn)行序列分析。