Design another set of specific primers, of which upstream and downstream primers were located in the two sides of the cleavage site respectively, to develop the one-step RT-PCR method for detecting H5 and H9 subtypes of AIV. Identify the sensitivity of the developed one-step RT-PCR method by detecting 2 AIV strains of H5 subtype and 3 AIV strains of H9 subtype, and the specificity by detecting the RNAs of NDV, IBV and IBDV as well as analogue detection using computer software.

根據(jù)H5视频黄页大全、H9亞型HA基因序列，分別設(shè)計一套特異性引物国产操B在线视频，其上黄色网站在观看18181818禁、下游引物分別位於裂解位點兩側(cè)，應(yīng)用RT-PCR一步法檢測AIV H5和H9亞型日韩一区二区A片免费观看，并驗證所建立方法的敏感性官产在线免费观看无码；通過檢測NDV、IBV和IBDV等RNA病毒及電腦軟件模擬檢測亚洲A片在线观看片不卡，驗證其特異性超碰人妻97精品。

Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法檢測免疫血清抗體對ED的親合力。2在线精品91青草国产在线观看、對DNA疫苗組小鼠制備OVA致敏哮喘模型三A黄色网站，檢測氣道壓力峰值—時間指數(shù)、支氣管／肺泡灌洗液中炎性細胞數(shù)量国产高清色色，PAS特染觀察小支氣管杯狀細胞數(shù)量及RT-PCR法檢測肺組織中粘蛋白MUC5AC mRNA水平免费看的黄色视频，免疫組化和RT-PCR法檢測GM-CSF的蛋白與mRNA水平。

A HIL simulation system based on concurrent computer is presented and the RT-Lab is applied to solve real-time control problem. Using the concurrent computer and RT-Lab, both anti-skid braking controller and hydraulic system can be put into the simulation loop.

提出了飛機剎車系統(tǒng)半實物仿真設(shè)計方案自拍视频最新日韩欧美，在并行計算機硬件平臺基礎(chǔ)上91亚洲精品自在在线观看不卡的，將實物(如剎車控制單元、液壓模擬系統(tǒng)等)接入仿真回路欧洲色国产欧美一区二区精品97，在RT-LAB環(huán)境下構(gòu)建了仿真平臺国内JIZZ。

Sensitivity comparison between the novel one-step RT-PCR and DAS-ELISA for PVX revealed that the former method was 20 times more sensitive than the later one. The novel one-step RT-PCR system was also successfully tested with field samples of turnip mosaic virus in 8 different crucifer vegetable crops.

為驗證該設(shè)想，本研究建立了一步RT-PCR技術(shù)擴增體系在线观看污色视频，并利用該體系擴增和分析了國內(nèi)幾種蔬菜作物部分CMV分離物的2b基因序列同源性亚洲精品福利视频，選擇特定分離物的全長2b基因，先利用PVX侵染性載體初步分析其正義和反義序列間的互作西安一级毛片，再構(gòu)建植物表達載體轉(zhuǎn)化煙草和番茄日韩视频一区二区，并研究轉(zhuǎn)基因煙草抗CMV的表現(xiàn)，獲得了抗CMV的轉(zhuǎn)基因煙草植株久久久精品区。

Soybean ; Bean pod mottle virus ; one step real-time fluorescent RT-PCR ; one step RT-PCR ; detection

大豆中文无码性爱；菜豆莢斑駁病毒人人摸人人看人人亲；一步法實時熒光RT-PCR ；一步法RT-PCR 日本女子婬荡视频一区二区三区；檢測

RT-3DE shows clearly full view of the primary atrial tumor than two-dimensional echocardiography and obvious image differences between atrial myxoma and malignant atrial tumor. It is a convenient, quick and precise modality for diagnosing primary atrial tumor and may be of significant clinical value for the diagnosis of primary atrial tumor.

RT-3DE比二維超聲心動圖更清晰顯示原發(fā)心房肺瘤全貌形態(tài)免费观看欧美日韩亚网红，黏液瘤與惡性心房腫瘤圖像特征差別明顯，RT-3DE是診斷原發(fā)心房腫瘤簡便日韩福利片影音先锋在线播放网站视频、快捷和準確的檢查方法鲁吧鲁吧鲁吧，RT-3DE對原發(fā)心房腫瘤具有重要臨床診斷價值。

Methods Semi-quantitative RT-PCR and real-time RT-PCR were applied to detect the expression level of PTX1 in 36 NPC and 8 chronic nasopharyngitis biopsies.

用RT-PCR和熒光定量RT-PCR檢測PTX1在36例鼻咽癌及8例慢性鼻咽炎中的表達国产国语对白Av在线。

Firstly,we isolated clinically and induced artificially multi-resistant Escherichia coli,detected antibacterials intake by different resistant level strains with CCCP and fluorescence spectro method and ascerained existing active efflux system in this strains; Secondly, construced internal standard of quantitive RT-PCR and detected AcrAB mRNA level of different resistant E.coli with quantitive RT-PCR; Thirdly, prepared AcrA antibody and detected AcrAB protein level of different resistant E.coli; eventually,designed and constructed Taqman probe of AcrAB and detected AcrAB level of different resistant E.coli with fluorescence quantitive PCR.

臨床分離及人工誘導(dǎo)大腸桿菌多重耐藥株97在线欧美超碰，氰氯苯腙結(jié)合熒光分光法檢測不同耐藥水平菌株對抗菌藥物的攝入，確證主動外排系統(tǒng)的存在久久久久久a亚洲欧洲AⅤ；構(gòu)建了定量RT-PCR的內(nèi)標準DNA三级片A片一级片，通過定量RT-PCR檢測動物源性大腸桿菌AcrAB mRNA的水平；制備AcrA抗體国产影视午夜在线观看视频免费下载，采用Western-Blotting檢測不同耐藥株AcrAB蛋白的表達水平又爽又黄又舒服的网站视频；設(shè)計和構(gòu)建AcrA、AcrB的Taqman探針精品国产AV最大网站下载，檢測不同耐藥菌株AcrAB的水平。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

應(yīng)用雜交瘤技術(shù)制備ZNRD1的首個單克隆抗體欧美色色色；2）利用RT-PCR羞羞视频在线登录页面免费视频入口页面破解、Western blot和免疫組化檢測ZNRD1在胃癌組織、胃炎組織在线一区二区观看、正常胃上皮組織av不卡在线、胃癌細胞和正常胃組織上皮細胞中的表達；3）構(gòu)建ZNRD1的小干擾RNA載體中文字幕专区，并測序鑒定激情综合丁香五月；4）利用脂質(zhì)體將ZNRD1的真核表達載體及其空載體轉(zhuǎn)染胃癌細胞（AGS、SGC7901青娱精品在线p、MKN28）和小鼠成纖維細胞（NIH3T3）成年美女黄网站18禁免费动漫，G418篩選后進行鑒定；5）利用脂質(zhì)體將ZNRD1的小干擾RNA載體及其空載體轉(zhuǎn)染藥敏胃癌細胞（SGC7901）中国一级免费A片、正常胃組織上皮細胞（GES-1）日韩精品在线看、對長春新堿耐藥的胃癌細胞（SGC7901/VCR）、藥敏白血病細胞（HL-60）18禁无码免费久久免费看、對長春新堿耐藥的白血病細胞（HL-60/VCR）国产18禁又污又黄又刺激，G418篩選后進行鑒定；6）利用MTT實驗檢測ZNRD1高/低表達對細胞（AGS国家一级A片一级毛片、SGC790191情侣在线精品国产免费、MKN28欧美不卡一区、NIH3T3、GES-1）生長的影響色呦呦在线播放；7）通過軟瓊脂克隆形成實驗檢測上調(diào)ZNRD1對AGS在线观看a片免费网站、MKN28細胞克隆形成能力的影響；8）通過裸鼠成瘤實驗檢測上調(diào)ZNRD1對AGSbbbxbbbxx、MKN28細胞體內(nèi)成瘤性的影響日韩精品国语在线观看免费视频网站；9）通過流式細胞儀分析ZNRD1高/低表達對細胞（AGS、MKN28在线免费AV播放、NIH3T3中日韩最新无码视频网站大全、GES-1）的細胞周期的影響；10）通過流式細胞儀分析上調(diào)ZNRD1對細胞（AGS欧美一级做一级A片视频大鸡巴破处、MKN28欧美18免进、NIH3T3）的凋亡的影響；11）通過MTT實驗檢測ZNRD1高/低表達對細胞（SGC7901插进去插快点视频、SGC7901/VCR黄色网站高潮视频免费观看、HL-60、HL-60/VCR）體外藥物敏感性的影響日韩欧美一区二区在线观看；12）通過腎包膜下移植法檢測ZNRD1高/低表達對細胞（SGC790118AV视频、SGC7901/VCR）體內(nèi)藥物敏感性的影響；13）通過流式細胞儀分析ZNRD1高/低表達對細胞（SGC7901色色www,com、SGC7901/VCR国产黄片亚洲欧美、HL-60、HL-60/VCR）內(nèi)阿霉素蓄積和泵出的影響97国产a片观看视频；14）通過透射電鏡檢測上調(diào)ZNRD1對SGC7901細胞凋亡敏感性的影響伊人色色网站；15）通過流式細胞儀和DNA梯度試驗檢測ZNRD1高/低表達對細胞（SGC7901、SGC7901/VCR丝袜激情在线一区、HL-60）凋亡敏感性的影響www.96；16）通過基因芯片檢測ZNRD1高/低表達對胃癌細胞內(nèi)基因表達譜的影響；17）利用RT-PCR无码,色,不卡,AAA、Western blot對基因芯片的結(jié)果進行鑒定日韩一级免费视频网站；18）利用報告基因?qū)嶒灆z測ZNRD1對cyclin D1的啟動子活性的調(diào)節(jié)作用；19）利用報告基因?qū)嶒灆z測ZNRD1高/低表達對MDR1的啟動子活性的調(diào)節(jié)作用尤物网站视频免费看；20）利用激酶試驗檢測ZNRD1對cyclin E-CDK2 激酶活力的影響人人操人人操操；21）利用反義核酸技術(shù)抑制p21的表達；通過流式細胞儀檢測抑制p21對ZNRD1介導(dǎo)的細胞周期阻滯的影響影音先锋中文字幕资源；22）利用反義核酸技術(shù)抑制p27的表達免费网站在线观看人成视频；通過流式細胞儀檢測抑制p27對ZNRD1介導(dǎo)的細胞周期阻滯的影響；23）利用脂質(zhì)體轉(zhuǎn)染法上調(diào)Skp2的表達久久riav.；通過流式細胞儀檢測上調(diào)Skp2對ZNRD1介導(dǎo)的細胞周期阻滯的影響国产羞羞视频在线观看；24）利用Western blot檢測ZNRD1對p27和Skp2的蛋白穩(wěn)定性的影響亚洲日韩色欧另类欧美在线蜜臀；25）利用微血管密度實驗檢測ZNRD1對AGS、MKN28細胞裸鼠移植瘤微血管形成的影響黄色片视频A急片；26）利用ELISA檢測ZNRD1對AGS国产一区二区视频免费看、MKN28細胞培養(yǎng)上清和移植瘤勻漿中VEGF165含量的影響；27）利用脂質(zhì)體轉(zhuǎn)染法色又色综合、MTT實驗精品国产丝袜在线拍国语APP、腎包膜下移植法、流式細胞儀和DNA梯度試驗檢測新耐藥相關(guān)分子DARPP-32對細胞（SGC7901综合视频一区、SGC7901/VCR99er久久免费只有精品、對阿霉素耐藥的胃癌細胞SGC7901/ADR）多藥耐藥表型的影響；利用脂質(zhì)體轉(zhuǎn)染法和MTT實驗檢測下調(diào)ZNRD1對DARPP-32介導(dǎo)的胃癌多藥耐藥的調(diào)控作用369超碰在线观看。

Methods: Using the ELISA and RT-PCR to detect Rotavirus and astrovirus respectively in stool of the 117 in-patient children diagnosed of enteritis in 2003. The astrovirus positive specimens tested by RT-PCR firstly were serotyped by RT-PCR secondly using the serotype-specific primers, the epidemiological and clinical data including age, season distribution, chief symptoms were compared and analysed.

分別對2003年收集的我院117例腹瀉患兒運用ELISA法檢測輪狀病毒及用RT-PCR法檢測星狀病毒日韩美女观看成人片在线播放网站，并應(yīng)用兩次擴增法對星狀病毒進行分型，并對其臨床及流行性學(xué)資料進行統(tǒng)計學(xué)分析国产一级av网址，包括患兒年齡自拍日韩在线视频播放，感染季節(jié)及主要臨床癥狀等。