Specific primers were designed according to gene NHX1 of Limonium gmelinii published on NCBI. Then, the gene was cloned by means of RT-PCR. Eukaryotic expression vector pCAM-BIA1301-NHX1 had been constructed, then transformed into tomato by Agrobacterium-mediated transformation method. Transgenic tomatos were obtained.

根據(jù)NCBI公布的大葉補血草NHX1基因序列設(shè)計特異性引物一本无码二区，經(jīng)RT-PCR方法克隆得到大葉補血草液泡膜Na/H逆向轉(zhuǎn)運蛋白基因NHX1，構(gòu)建植物融合表達載體pCAMBIA1301-NHX191po精品国产，重組質(zhì)粒通過農(nóng)桿菌介導(dǎo)轉(zhuǎn)化番茄，經(jīng)PCR和RT-PCR鑒定高清狼友网，獲得轉(zhuǎn)基因番茄植株91在线国产视频观看。

To eplore the relationship between Dynein and the formation of meiosis error of oocytes, the immature mouse oocytes were in vitro cultured with a known dynein ATPase activity inhibitor- sodium orthovanadate to detect the changes of maturation rate, and semi-quantitative RT-PCR and single cell RT-PCR were performed to detect the alteration of the cyclin B1 mRNA level.

為探討Dynein與卵母細胞減數(shù)分裂錯誤形成的關(guān)系，采用小鼠卵母細胞體外培養(yǎng)成熟技術(shù)和半定量RT-PCR方法成都毛片，分別檢測Dynein抑制劑-原釩酸鈉對卵母細胞成熟率和cyclin B1基因轉(zhuǎn)錄水平的影響国产精品亚洲獵奇在线观看。

Cells were immediately fixed and processed for immuno?uorescence and tested for translocation ofβ-catenin.β-catenin and target genes of Wnt/β-catenin signaling COX-2, cyclinD1, c-fos, c-Jun mRNA expression were examined by RT-PCR. Saos-2 osteoblastic cells were co-transfected with a wild type or mutant Tcf luciferase reporter gene and Renilla luciferase plasmid. Posttransfection cells were subjected to 1 hour of 12% elongation.

作用1h、2h和4h后黄页三级，用免疫熒光的方法檢測β-catenin的表達和在細胞上定位的變化人妖色偷偷福利网站；用半定量RT-PCR檢測β-catenin mRNA表達的變化；用質(zhì)粒轉(zhuǎn)染的方法檢測熒光素酶報告基因Tcf轉(zhuǎn)錄的情況欧美性爱一区二区三区精品视频；用半定量RT-PCR檢測Wnt/β-catenin通路下游基因COX-2国产一二区精品，cyclinD1， c-fos久久久性爱免费视频， c-Jun mRNA表達的情況在线观看A片噜噜操大全；用免疫共沉淀的方法檢測β-catenin和E-Cadherin的結(jié)合情況。

In order to establish the detection of Hemagglutinating Encephalomyelitis Virus by RT- PCR, a pair of oligonucleotide primers appropriate for PCR amplication was selected based on the HE gene and S gene of HEV.

為了建立快速檢測豬血凝性腦脊髓炎病毒的RT-PCR方法亚洲欧美精彩视频，根據(jù)GenBank中HEV的HE基因和S基因設(shè)計了1對引物久久国产高潮流白浆A片，在優(yōu)化RT-PCR反應(yīng)條件的基礎(chǔ)上，成功的擴增出323bp的特異性條帶www.av天堂2018.com。

METHODS: Sixty two cases of specimens collected in operation were studied by in situ RT PCR, of which 50 cases were hemangioma: 26 cases proliferating hemangioma and 24 cases involuting hemangioma, and 12 cases were venous vascular malformation. The expression of Ang2 mRNA and Tie2 mRNA in hemangiomas and venous vascular malformation were detected by in situ RT PCR.

采用原位RT PCR方法欧美黑人操B巨大，對臨床收集的62例手術(shù)切除的先天性皮膚血管標(biāo)本（其中增生期血管瘤26例，消退期血管瘤24例欧美日韩性爱小说视频，靜脈型血管畸形12例）進行Ang2 mRNA欧美一级爽AAAAA片在线看， Tie2 mRNA表達水平的檢測。

Esults LVSV and RVSV determined by RT-3DE were (15.90±10.62) ml and (12.42±4.69) ml respectively, which were shown good correlation with those determined by MRI [(16.56±10.64)ml and (13.96±7.96) ml]. The r value was 0.98, and 0.75, respectively. LVSV and RVEV determined by RT-3DE were (12.67±10.10) ml and (11.83±12.02) ml respectively, which were well correlated with MASE (8.17±1.92)mm and TASE (11.04±1.06) mm determined with ME. The r value was 0.86 and 0.88 respectively.

T-3DE測定左室每搏量(12.67±10.10)ml和ME測定二尖瓣瓣環(huán)收縮期平均位移(8.17±1.92)mm之間的相關(guān)性顯著A级黄黄色网站，相關(guān)系數(shù)r=0.86(P.001)久久久久久精品毛片A级；RT-3DE測右室每搏量(11.83±12.02)ml和ME測定三尖瓣瓣環(huán)收縮期位移(11.04±3.06)mm兩者亦存在顯著正相關(guān)，相關(guān)系數(shù)r=0.88(P.001)WWW免费簧片AV网站COM。

CEVd is a non-polyadenylated RNA viroid,but there is a fragment rich in A in CEVd sequence.On the bases of uniplex RT-PCR, a multiplex RT-PCR was developed to detect CTV,CEVd and CTLV simultaneously.

實驗過程中亚洲久色，發(fā)現(xiàn)不具有ploy_加尾的環(huán)狀類病毒CEVd，同樣可以用oligo反轉(zhuǎn)錄的RT-PCR檢測出來鲁一鲁中文字幕www，通過測序分析在线看手机毛片，其同源性在96%以上，并發(fā)現(xiàn)在CEVd序列中有一個富含A的區(qū)段影视先锋av在线资源。

Objective To establish a competitive RT-PCR method for quantitative determination of IFN-γ mRNA and IL-4 mRNA and explore the optimal inductive condition in vitro. Methods IFN-γ mRNA and IL-4 mRNA in PBMC induced by PMA and calcium ionophore of a healthy volunteer and in unstimulated PBMC of 11 HVs and 13 asthma patients were determined quantitatively with the established competitive RT-PCR method using self-prepared internal standard as competitive templets.

Subject words 】 Competitive templet亚洲一区有码在线； IFN-γ； IL-4； mRNA日韩欧美综合在线； RT-PCR 檢測細胞因子最早使用生物學(xué)或免疫學(xué)測定法久久精品99视频在这里观看视频，這兩類檢測可反映某種細胞因子分泌、吸附AAA级黄片、消耗和降解后的凈含量〔1〕黄色视频网，但不能為細胞因子產(chǎn)生過程中基因轉(zhuǎn)錄、翻譯及翻譯后調(diào)控機制提供必要的信息A女人黄色网站，也不能反映細胞或組織內(nèi)細胞因子基因表達水平亚洲中文字幕在线第一页。

The same 20 viruses have been previoushy typed using serotyping method resulting that only 11 could be typed successfully, showing a high consistency with that of RT-PCR-RFLP method and a 30% lower typing efficiency than the latter.

此20個毒株曾用血清學(xué)方法分型，僅11株分型成功91九色国产，與RT-PCR-RFLP法結(jié)果符合国产激情网在线视频。分型成功率RT-PCR-RFLP法比血清法高30%。

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

以牛泡沫病毒(Bovine foamy virus久久久在精品视频， BFV)中國株BFV3026原病毒DNA為材料亚洲一区二区5566在线，構(gòu)建R區(qū)系列缺失質(zhì)粒，通過對其轉(zhuǎn)染細胞中RT水平及對缺失質(zhì)粒與luc報告質(zhì)粒共轉(zhuǎn)染細胞中螢火蟲熒光素酶活性的測定女人黄图视频图片，確立U5區(qū)對于BFV3026兩類啟動子LTR和IP均具有負調(diào)控作用91人洗澡人人澡人人爽人人模；同時將帶有不同R區(qū)的BFV3026結(jié)構(gòu)基因片段克隆于異源啟動子CMV之下，通過對其轉(zhuǎn)染細胞293T中RT酶活性的測定91日韩精品一区二区亚洲专区，確立R區(qū)對于病毒結(jié)構(gòu)基因pol的表達具有一定的調(diào)節(jié)作用97无码免费人妻视频，并將其功能區(qū)域初步界定在R區(qū)5′端100bp內(nèi)。