Using Cassia tora as material,a cDNA encoding chalone synthase was cloned from tender leaves of Cassia tora by RT-PCR and RACE.The full-length cDNA of CHS from Cassia tora had 1 459 bp with an open reading frame encoding 390 amino acids of protein.

以決明為實驗材料，利用RT-PCR和RACE技術(shù)亚日韩欧美性爱片，從決明嫩葉中克隆出查爾酮合成酶(Chal-one synthase国产又大又硬又爽免费视在线观看，CHS)基因欧美一级A片在线视频免费观看，其cDNA全長為1 459 bp，編碼一個由390個氨基酸殘基組成的多肽我要操我要干。

Main methods used in this study include immunohistochemistry, Laser Confocal Scanning Microscope , cell ELISA, RT-PCR and cell chemotaxis.

實驗方法主要包括：免疫組化午夜免费播放成人无码视频在线看、激光共聚焦、細(xì)胞ELISA国产一级性爱网站、RT-PCR和細(xì)胞趨化實驗等黄色自拍偷拍网址。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling . Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied.

在研究過程中，采用了病毒接種技術(shù)免费AA午夜精品、普通病理學(xué)研究方法免费美女网站国产、流式細(xì)胞儀技術(shù)、RT-PCR技術(shù)晚上福利视频、原位雜交技術(shù)人人看人人摸人人操人人看、免疫組化技術(shù)、凋亡細(xì)胞末端標(biāo)記技術(shù)国产A片综合，系統(tǒng)研究了不同日齡的SPF雛雞人工感染AEV-NH937株后的病理變化最新2020中文字幕永久人人视频，T和B淋巴細(xì)胞、T淋巴細(xì)胞亞群的動態(tài)變化規(guī)律搞黄刺激网站，淋巴細(xì)胞表面某些粘附分子的變化欧美三级国产一级，淋巴細(xì)胞和神經(jīng)元的壞死與凋亡，雛雞腦組織中產(chǎn)生趨化因子MIP-1β和IL-8細(xì)胞的變化規(guī)律国产免费夜间在线观看AV，腦組織中圍官性細(xì)胞浸潤的細(xì)胞類型。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究過程中成人在线免，采用了病毒接種技術(shù)欧美激情四射三级网站、普通病理學(xué)研究方法、流式細(xì)胞儀技術(shù)女人一级片免费、RT-PCR技術(shù)欧美真人性爱一线黄片在线免費观看、原位雜交技術(shù)、免疫組化技術(shù)凹凸性爱视频、凋亡細(xì)胞末端標(biāo)記技術(shù)看黄网站免费，系統(tǒng)研究了不同日齡的SPF雛雞人工感染AEV-NH937株后的病理變化，T和B淋巴細(xì)胞Av最新高清无码专区、T淋巴細(xì)胞亞群的動態(tài)變化規(guī)律看黄女AV网站，淋巴細(xì)胞表面某些粘附分子的變化，淋巴細(xì)胞和神經(jīng)元的壞死與凋亡点击进入在线影院毛片，雛雞腦組織中產(chǎn)生趨化因子MIP-1β和IL-8細(xì)胞的變化規(guī)律成视频在线一区精品免费，腦組織中圍官性細(xì)胞浸潤的細(xì)胞類型。

Semi-quantitative RT-PCR analysis showed that ZF1 expressed in root, stem, leaf, flower, immature pod, and embryo of chickpea with different expression patterns.

半定量RT-PCR分析表明一级毛片AAAA视频播放，ZF1在鷹嘴豆的根18禁黄黄网站在线看、莖色永久免费视频入口、葉、花草民网理论片看一天717影院、幼莢和幼胚中均有表達(dá)国产亚洲欧美日韩在线观看不卡，在莖和葉中表達(dá)較弱，為組成型轉(zhuǎn)錄因子两个黄片毛片。

Methods: We extract hippocampus chondriosome from the vascular dementia rat and measure the activity of mitochondrial COX by the UV-spectrophotometer, then detect its gene order through RT-PCR.

從VD大鼠中提取海馬線粒體利用紫外分光光度計技術(shù)檢測各組大鼠海馬COX活性人人操,人人揉,人人看，通過RT-PCR等技術(shù)對其基因序列進行檢測。

PCR, RT-PCT and PCR-Southern blot analysis indicated that phyB gene was successfully integrated into citrange genome and was transcribed in citrange.

PCR涩涩视频在线观看入口、RT-PCR和PCR Southern雜交結(jié)果表明：phyB基因已整合到枳橙的基因組国产吧在线视频永久，并得到了轉(zhuǎn)錄；轉(zhuǎn)phyB基因枳橙表現(xiàn)出植株矮化亚洲A级性爱视频、分枝角度加大国厂熟女a乱又伦、葉片變小、比葉干重增加免费黄色电影一级，其葉綠素含量比對照高29.1％三级片在线免费。

The mRNA of pituitary glands from seven important economic Pleuronectiformes fishes was extracted,the cDNA sequences of GH gene were then isolated from Cleisthenes herzensteini,Limanda yokohamae,Pleuronichthys Cornutus,Cynoglossus robustus,Platichthys bicoloratus, Zebrias zebra and Paralichthys dentatus with RT-PCR method by using designed specific primers based on the reported GH cDNA sequences of Pleuronectiformes. The results of sequencing showed that lengths of the above cDNAs are as follows: 479 bp,564 bp,519 bp,440 bp,564 bp,440 bp and 522 bp.The GH cDNA sequences encode mature polypeptide of about 140 to 170 amino acid.The full-length growth hormone cDNAs of Paralichthys lethostigma and Scophthalmus maximus were cloned by Switching Mechanism At 5\' end of the RNA Transcript RACE technology.

從7種重要鰈形目經(jīng)濟魚類——高眼鰈、黃蓋鰈超乳爆乳在线观看免费、木葉鰈欧美激情视频91、寬體舌鰨、石鰈和條鰨机机干免费在线视频、夏鲆的腦垂體中提取mRNA91视频官方，并采用RT-PCR方法，根據(jù)已克隆得到的鲆鰈魚類生長激素基因的保守性序列設(shè)計特異性引物网址性av，克隆了含開放閱讀框的生長激素(Growth Hormone日韩专区视频，GH)cDNA序列，測序結(jié)果顯示日本高清视频在线观看Aⅴ，高眼鰈亚州+日韩+国产+无码、黃蓋鰈、木葉鰈怕怕网站免费观看、寬體舌鰨免费A片视频小草、石鰈、條鰨和夏鲆的GH cDNA長度依次為479 bp国产高清在线视频一区二区、564 bp久操依人、519 bp、440 bp男人的天堂综合网、564 bp小度小度找个黄片看看、440 bp和522 bp，編碼140-170個氨基酸的成熟GH多肽片段；從漠斑牙鲆和大菱鲆腦垂體中提取mRNA黄色xxxx视频，利用SMART-RACE技術(shù)建立腦垂體cDNA文庫男人的精品在线观看，并從該文庫中克隆出大菱鲆和漠斑牙鲆生長激素基因cDNA全長序列。

For the time being,the NDV detection of CIQ includes cloaca tampon separate virus,ELISA,HI and RT-PCR is trying now.

目前我國出入境檢疫中對新城疫病毒的檢測方法主要有瀉殖腔棉拭子樣品病毒分離鑒定无码三级在线中文字幕、HI和ELISA精品毛片av一区二区在线，RT-PCR檢測技術(shù)正在試用之中。

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我們還用RT-PCR方法檢測了Centrin同源基因在單環(huán)刺螠精子分化過程和早期胚胎發(fā)育過程的表達(dá)美日韩制服中文字幕视频，結(jié)果顯示：在單環(huán)刺螠精子發(fā)生過程中的精細(xì)胞精品狼友视频在线播放、精子團、精子中都有表達(dá)毛片无码大全免费，而在成體的體壁組織人妻大白腚、消化道組織、體腔球中則沒有表達(dá)www.xx日本黄色视频.com；在未受精卵中沒有表達(dá)国产淑女色视频，而在受精后釋放極體的受精卵和經(jīng)A23187孤雌激活的卵中則有表達(dá)；在單環(huán)刺螠的早期發(fā)育過程——二細(xì)胞胚胎日韩欧美女自拍图片大全集高清视频、多細(xì)胞胚胎15级黄片电影、原腸胚、早期擔(dān)輪幼蟲——中都有表達(dá)欧美A片一级在线观看嗯啊。