A 3-1E gene fragment was amplified from Eimeria acervulina SH strain sporozoite by RT-PCR,and it was cloned into eukaryotic expression vector pcDNA3.1 to construct the recombinant plasmid pcDNA3.1-3-1E.

應(yīng)用反轉(zhuǎn)錄-聚合酶鏈式反應(yīng)技術(shù)從堆形艾美球蟲SH株子孢子中擴增3-1E基因片段，將其克隆入真核表達載體pcDNA3.1中野外性XXⅩXX大少妇，構(gòu)建重組表達質(zhì)粒pcDNA3.1-3-1E。

The result of RT-PCR showed that MuMADS1 just expressed in the flower and fruit but not in the root、 stem and leaf.

根據(jù)其表達量的變化欧美中日韩免费专区在线，推測MuMADS1可能除了花的發(fā)育起作用外，在果實發(fā)育的早期與果實的發(fā)育相關(guān)美女免费黄网，而在果實發(fā)育的晚期與成熟相關(guān)www.色综合.com。

Methods: 2 hours of reversible focal ischemia in rats were produced by improved the method of Connolly' suture occlusion of MCA and the expression of SAMDC-mRNA in the cortex and subcortex was measured by RT-PCR after 2,4,8 and 24h reperfusion following 2h ischemia respectively.

在Connolly線栓法的基礎(chǔ)上進行改良，復制大鼠MCA區(qū)2h腦缺血-再灌流2欧美打性爱在线，4欧美成电影网站色www，8，24h動物模型色综合五月天，用逆轉(zhuǎn)錄多聚酶鏈反應(yīng)測定缺血區(qū)皮質(zhì)和皮質(zhì)下組織不同時相SAMDC-mRNA的表達www,AV天天27。

In this study,the full length cDNA of nAChR α subunit from this pest was cloned by RT-PCR.

序列分析表明，這是 1個新的α亞基基因六月长期国产对白视频，定名為Csα 199re6这里视频精品在久线30。

Results: Mdroxyprogesterone acetate plus E2 (10-8 M) stimulated both the mRNA levels and activities of sulfatase (at 10-8-10-6 M) compared to E2 only.

用同位素標記的底物和RT-PCR技術(shù)分別測定硫酸酯酶和硫轉(zhuǎn)移酶的活性和mRNA表達水平日韩精品国语在线观看。

The cell supernatant was collected after 3 and 6 days.

RT-PCR檢測細胞Ⅱ型膠原和aggrecan的mRNA表達水平。

The levels of plasma and myocardium of malondialdehyde, superoxide dismutase and cAMP were measured.

原位雜交及RT-PCR觀察ADM mRNA在各組心肌表達的變化国内一级毛片免费不卡视频。

The frequency of losing exon 5-8 was the highest . It was consistent with the results of the FHIT gene whole length cDAN by RT-PCR.Conclusions: The above results showed that the changes of FHIT gene is probably related to the development of kinds of tumors, which is an important molecular event. FHIT gene can be considered to be a tumor suppresser gene, our studys provided a theoretic and experimental data for further research on the function of FHIT gene.

結(jié)論本研究結(jié)果初步證明FH工T基因改變可能與多種腫瘤的發(fā)生有關(guān)影音先锋女人鲁色资源网，是腫瘤發(fā)生的一個重要的早期分子事件，支持其作為一個抑癌基因在腫瘤發(fā)生中發(fā)揮作用尤物网视频在线，為進一步深入研究FH IT基因的功能提供了理論和實驗依據(jù)欧美a片网止。

Materials and Methods : From January 1996 to December 2003, 92 lung cancer patients with SVC syndrome were referred for RT.

材料與方法：從 1996 年 1 月到 2003 年 12 月，我們接受了 92 位原發(fā)性肺癌合併上腔靜脈癥候群的病患的會診而進行放射治療高清在线看欧美亚洲中文字幕。

AIM: To isolate bioactive secondary metabolites from the fruits of Schisandra rebriflora Rehd. et. Wils and study their effects on HIV-1 infectivity. METHODS: Inhibition of syncytia formation, HIV-1 reverse transcriptase and protease, protection of HIV-1 infected cells, level of HIV-1 p24 antigen,blockage of fusion were detected. RESULTS: A lignan compound, named rubrifloralignan A, was first isolated as a natural product with modest inhibition effects on syncytium formation and HIV- 1 replication and could protect HIV-1 infected cells with weak inhibition effects on fusion and RT, but it showed no effects on the replication of HIV-1 chronic H9 and PR. CONCLUSION: Rubrifloralignan A is an efficient anti-HIV-1 product with effect on early stage of HIV-1 replication.

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