Methods With β-actin gene as a reference, reverse transcriptase polymerase chain reaction was conducted to dectect the expression of PSA mRNA in 35 BC samples, 12 paracarcinoma breast tissues and 10 breast adenofibroma samples.

以β actin作為內(nèi)部參照半定量RT PCR方法檢測(cè) 35例乳腺癌組織www.色色网、12癌旁乳腺組織和 10例乳腺纖維瘤中PSAmRNA的表達(dá)国产一级A爱片在线视频。

It indicated that in rape, six of ABCD genes that are Apetalal, Apetala2, Apetala3, Pistillata, Agamous and Agamous-like11, exhibited a variation in the expression level at the different development stages.

以油菜處于花蕾發(fā)育過(guò)程7個(gè)生長(zhǎng)期的花蕾和花朵為材料欧美日本大片在线观看黄，利用優(yōu)化的半定量RT-PCR技術(shù)在轉(zhuǎn)錄水平對(duì)ABCD模型的6個(gè)功能基因，即Apetalal国产免费观看黄页影视网站在线观看、Apetala2欧美A以一区二区三区、Apetala3、Pistillata女人一级A片免费视频、Agamous18禁黄网站无码无遮挡免费、Agamous-likell，進(jìn)行分析禁止18观看，探討了油菜花蕾發(fā)育及開(kāi)花過(guò)程中6個(gè)功能基因表達(dá)的差異欧美18禁止看网站。

We cloned the Rana temporaria chensinensis David skin cDNA using a PCR strategy and detected its activity in rana using a antibacterial protein assay against Micrococcus luteus. The cloning was based on a report of GenBank. The deduced amino acid sequence resulted in 53 amino with 46% identity to amphibia. RT-PCR was used to detect peptide mRNA in Rana temporaria chensinensis David.

我們用提取中國(guó)林蛙皮肽總RNA，逆轉(zhuǎn)錄合成cDNA精品亚洲护士av无码番号，PCR擴(kuò)增得到DNA片段精品一级A片中文字幕，并與GenBank上登陸的其他蛙類的皮肽抗菌肽序列進(jìn)行比較，發(fā)現(xiàn)此序列是一個(gè)新的蛙皮肽抗菌肽序列A级毛片高清免费视。

RT-PCR amplification of ginseng β-amyrin synthase gene was successfully performed based on the total RNAs extracted from ginseng hairy roots induced by Agrobacterium rhizogenes A4 using a modified guanidine isothiocyanate-method.

以發(fā)根農(nóng)桿菌A4菌株誘導(dǎo)的人參發(fā)根為材料A级毛片全部免费高清视频人人，用改良的異硫氰酸胍法提取總RNA，得到了純度好的完整的總RNA欧美性色视频。

Expression of a novel antra????poptosis gene, survivin, correlated with tumour cell apoptosis and p53 accumulation in gastric carcinomas［J］.

myc在癌旁正常食管黏膜組織中均未見(jiàn)表達(dá)口爆在线，與多數(shù)文獻(xiàn)報(bào)道一致［1， 7］91自啪。survivin為新發(fā)現(xiàn)的凋亡抑制基因，其在食管標(biāo)本中的研究較少婷婷色五月开心综合先锋影音，SARELA等［8］及KATO等［9］均用RTwww777miqi？？123区无码乱伦？亚洲不卡无码精品一区二区？

Maternal TGFαmRNA was gradually reduced from oocyte to 8-cell stage and maintaining lower TGFαmRNA level was to morula stage, assumably TGFαmRNA transcription was begun from 8-cell stage with the zygote genome activation.

EGF、TGFα和EGFR mRNA在生殖系統(tǒng)中的表達(dá)通過(guò)RT-PCR的方法檢測(cè)了EGF男女八x八x视频网站、TGFα和EGFR mRNA在生殖系統(tǒng)中的表達(dá)国产999国内精品视频免费。

METHODS: 40 rats were randomly divided into four groups:normal group, asthenic spleen group, high-dosage group and low-dosage group with 10 rats in each group, the asthenic spleen rat models were established in group B, C group as well as group D. Rats in group B, group C, group D were fed with normal distilled water, high-dosage of Guizhi decoction and low-dosage of Guizhi decoction respectively, NFAT mRNA, IL-4 mRNA, IFN-γmRNA were determined by PCR.

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METHODS: Gastric carcinoma cell line MKN45 at logarithmic growth phase was subcutaneously inoculated in 20 athymic mice.

將對(duì)數(shù)生長(zhǎng)期的胃癌細(xì)胞株MKN45接種到裸鼠皮下欧美黄色网站有哪些，成瘤后用制備好的分別含GCRG213正99热精品在线免费观看、反義克隆和RNAi片段的腺相關(guān)病毒分別在腫瘤局部注射，以空病毒和生理鹽水對(duì)照日韩快播自拍图片在线观看，繼續(xù)喂養(yǎng)2 wk后處死工口无遮挡免费视频网站，取腫瘤測(cè)量重量，并用半定量RT-PCR檢測(cè)腫瘤組織GCRG213mRNA表達(dá)水平96人人操。

Methods］ Healthy SD rats were divided randomly into 6 group, including: normal control group(sham-operation), ischemia for 45 min (reperfusion 0h, n=6), reperfusion 2,4,12 and 24 hr group. Renal ischemia reperfusion injury models were founded in other five groups, opened abdominal and placed atraumatic vascular champ in dual kidney pedicle for 45 minutes,followed by reperfusion 2,4,12 and 24 hr.

方法］ 將SD大鼠隨機(jī)分成正常對(duì)照組50岁肥熟丰满熟妇在线播放、單純?nèi)毖M、再灌注2 h組免费av在线不卡澳门、4 h組成年人在线、12 h組、24 h組红豆视频直播免费观看在线，制備雙側(cè)腎蒂夾閉模型后一级欧美D，腹主動(dòng)脈取血測(cè)血肌酐、尿素氮国产黄色视频免费看日韩黄色视频免费看，行腎臟組織病理學(xué)檢查亚洲卡通动漫激情另类，免疫組化法檢測(cè)TGFβ1 蛋白，RT-PCR法檢測(cè)TGFβ1 mRNAwww.高清色色视频.cnm。

To establish the base of the control of the Citrus aurantium L. fruit maturity in molecular level and produce high quality Fructus Aurantii, a pair of degenergte primers were designed according to the conserve regions of ACC Oxidase gene of many kinds of plants.

根據(jù)已報(bào)道的各種植物ACC氧化酶氨基酸序列上前后兩個(gè)保守區(qū)設(shè)計(jì)1對(duì)簡(jiǎn)并引物色网视频A，以酸橙葉片總RNA為模板，通過(guò)RT-PCR擴(kuò)增到1個(gè)834bp的基因片段717午夜理论片在线观看影片。