Semi-quantitative PCR analysis showed it was expressed in each phase of Jatropha curcas growth.

半定量RT-PCR研究表明該基因在植株的整個生長發(fā)育期都有表達(dá)国产盗摄无遮挡裸体视频。

In this study, the complete coding sequence of N-hydroxylating cytochrome P450 (CYP79D2) gene, which catalyzing the committed enzyme in the first step in the biosynthesis of cyanogenic glucoside, was cloned by RT-PCR from cassava.

根據(jù)國際數(shù)據(jù)庫中的木薯氰化物合成限速酶基因序列信息，設(shè)計特異引物欧美特级一级黄色视频，通過同源克隆法免费人成A片在线视频，從木薯中克隆出1626bp cDNA基因序列。

At the same time the cell cycles of each group were determined by means of flow cytometer.

采用RT-PCR技術(shù)檢測神經(jīng)干細(xì)胞分化過程中GSK-3β超碰人人爽爽人人高潮喷水、CDK-5和PP2A的mRNA表達(dá)91色老久久精品偷偷鲁无毒。

The morphological and cytopathological characters of the viruses were observed by electron microscopy The types of virus were identified with I-ELISA, DAS-ELISA, TAS-ELISA, RT-PCR and IC-PCR. The CP genes of the main viruses identified in this research were amplified by PCR, cloned into pGEM-T and sequenced. The pumpkin germplasm from Heilongjiang and Yunnan provinces was screened for resistance to the main viruses using friction and roots immersed inoculation.

應(yīng)用電子顯微鏡負(fù)染法和超薄切片法觀察病原粒體形態(tài)及細(xì)胞病理學(xué)特征；利用酶聯(lián)免疫吸附測定法中的雙抗體夾心ELISA,,,亚洲视频在线观看、間接ELISA亚洲性夜夜综合久久麻豆、三抗體夾心ELISA以及反轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)、免疫捕捉PCR方法鑒定了采集樣品中的病毒種類亚洲精品欧美激情专区；對被確定為南瓜主要病毒病原的外殼蛋白基因進(jìn)行了PCR擴增未满进入的黄色网站，克隆到pGEM-T載體并進(jìn)行測序；應(yīng)用摩擦接種法和浸根接種法對云南禁止18以下观看在线网站、黑龍江省部分南瓜品系一级作爱视频无遮拦、品種進(jìn)行了抗性篩選。

Consistence to the PT-PCR result, TLR4 protein was strongly expressed at syncytotrophoblast of placenta, cytotrophoblast of chorion and epithelial cell of amnion of pregnancy complicated with syphilis by Immunohistochemistry.

TLR4在妊娠合并梅毒感染的胎盤合體滋養(yǎng)細(xì)胞城中村按摩一区二区在线观看、羊膜上皮細(xì)胞和胎膜中的平滑絨毛膜細(xì)胞滋養(yǎng)細(xì)胞上表達(dá)增強欧美A片一级免费黄色视频，與RT-PCR結(jié)果一致中文字幕精品视频在线观看。

In sham-operation group,animals were decollated and their brains were taken out at 24 hours postoperatively. In all the groups, hippocampal brain injury was detected using histopathological method combined with semi-quantified RT-PCR methods for detecting caspase-3 mRNA. Adult rats aged 90 days were used in modified y maze to examine their learning and memory functions. All these three experiments were independent.

各組海馬區(qū)腦損傷行組織病理學(xué)檢測，半胱氨酸天冬氨酸蛋白酶-3 mRNA的表達(dá)采用半定量反轉(zhuǎn)錄-聚合酶反應(yīng)方法進(jìn)行檢查国产美女精品色好看，成年90 d齡的大鼠進(jìn)行三等分迷宮測試其學(xué)習(xí)記憶能力97网友上传精品免费视频，3個實驗各自獨立。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人類T淋巴細(xì)胞白血病細(xì)胞系Jurkat作為研究對象美女在线A级毛片，采用MTT法檢測細(xì)胞增殖活性超碰公开在线久；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI雙標(biāo)法檢測細(xì)胞凋亡日本黄色网站进入入口；流式細(xì)胞術(shù)黄色视频图片一区、Western Blot、RT-PCR檢測魚藤素作用前后欧美黄色免费网站视频，Jurkat細(xì)胞內(nèi)核孔蛋白Nup88国产在线视频。、Nup214與核磷蛋白(nucleophosmin， NPM)表達(dá)水平的變化欧美动日韩中文；激光共聚焦顯微技術(shù)觀察上述核孔蛋白與核磷蛋白的亞細(xì)胞定位情況男女综合网。

AIM: To investigate the change of cell cycle checkpoint kinases Chk1/2 in human gastric cancer MGC803 cells induced by diallyl disulfide. METHODS: mRNA expression was assayed by means of RT-PCR in MGC803 cells treated with DADS.

目的：觀察二烯丙基二硫化物對人胃癌MGC803細(xì)胞的細(xì)胞周期檢查點激酶1(Chk1)和細(xì)胞周期檢查點激酶2(Chk2)的影響。

Denn dich erkennt und h rt man nicht!

因為無人會認(rèn)出你欧美一级网址，傾聽你最刺激的性毛片免费视频！

In additional, eIF5a2 is located on chromosome 2B using Langdon D-genomic disomic substitution lines and the expression of eIF5A was also investigated by Semi-quantitative PCR.

進(jìn)一步研究表明eIF5a2位于2B染色體上，并用半定量RT-PCR 研究了小麥eIF5A基因的表達(dá)情況玖玖爱网。