Methods The mRNA level of tissues of 39 cases of breast cancer and 14 cases of fibroadenoma was measured by RT-PCR technique and its correlation was analyzed.

抽取乳腺腫瘤組織總RNA久久精品国产一区二区三区，采用半定量逆轉(zhuǎn)錄－聚合酶鏈反應(yīng)檢測39例乳腺癌和14例乳腺纖維腺瘤組織中RECK-mRNA和MMP9-mRNA的表達，分析其關(guān)系国内外激情视频网。

The 7.4 kb DNA fragment containing the S. Choleraesuis std fimbrial gene cluster was PCR amplified and cloned into the pCR-XL-TOPO vector. The open reading frames of the std fimbrial gene cluster were analyzed. The resulting recombinant DNA was transformed into Escherichia coli HB101 strain.

本實驗室藉由PCR方式一级大片黄，增幅7.4 kb的std線毛基因組，將其選殖至pCR-XL-TOPO質(zhì)體DNA久久精久久精久久精99，委外定序后一级精品一区视频在线观看，分析std線毛基因組之開放性讀框，進而將此重組DNA送入大腸桿菌HB101人人大香草av淘宝av线路一，并藉由RT-PCR及西方點墨試驗觀察到S欧美熟女乱交。

To investigate the function of STT3a gene in salt adaptation and flagellar regeneration of Dunaliella salina, a pair of degenerate primers was designed according to conserved homologous amino acid sequences of VCVFTA and DVDYVL of STT3a from Chlamydomonas, Arabidopsis thaliana and other organisms. A cDNA sequence of 1 650 bp encoding a whole functional domain of STT3a was amplified from D.

為了研究STT3a基因在杜氏鹽藻耐鹽及鞭毛再生方面的作用，根據(jù)衣藻国产黄精品、擬南芥等STT3a蛋白的氨基酸高度保守序列VCVFTA、DVDYVL設(shè)計一對簡并引物97综合干视频，采用RT-PCR及3'RACE的方法擴增杜氏鹽藻STT3a蛋白功能結(jié)構(gòu)域的cDNA序列成年人免费看的视频大全。

METHODS: The expression of GATA- 3 gene was analyzed by using RT - PCR - ELISA in BMSCs from 34 normal cases and 9 cases with AA.

目的：了解轉(zhuǎn)錄因子GATA-3基因在再生障礙性貧血和正常骨髓基質(zhì)細胞中表達的情況，探討AA發(fā)病的免疫機制heisi.com。

However, the binding patterns between the HU-induced and uninduced HEL cells were different. The data in Southwestern-blot analysis showed that the molecular weights of the GATA factors binding to the HS2core DNA sequence were 43kd and 47kd respectively.

反義寡核苷酸技術(shù)及RT-PCR的結(jié)果表明国产黄色A片网站，GATA-1的反義核苷酸抑制了羥基脲誘導(dǎo)的β-珠蛋白基因的表達，進一步證明了GATA-1因子在羥基脲誘導(dǎo)的HEL細胞分化過程中是不可缺少的促進因素999黄色网站。

The expression of Gekko Fstl1 was analyzed by northern blot, in situ hybridization and reverse transfer polymerase chain reaction.

通過RT-PCR方法研究Fstl1基因在壁虎主要組織中的表達分布與壁虎斷尾損傷模型中變化欧美性爱一区视频。

METHODS: RT-PCR was used to observe the expressions of AT2 mRNA in the cortex of rat kidney, including neonate rats (1 d), mature rats (8 weeks) and gerontic rats (20 months).

分別觀察新生(1d)、成年(8wk)及老年(20mo)3組大鼠腎臟皮質(zhì)中AT2mRNA的表達及其增齡性變化日韩精品深夜666片免费观看网站，并測定腎皮質(zhì)中AngⅡ水平另类图片一区二区三区。

Methods MTT assay for detection of single use of Adriamycin and combination of Adriamycin with gleditsia saponin on the Role of the SMMC-7721 cell survival rate; detection of mRNA expression of bcl-2 by PCR method; detection of protein expression of bcl-2 by Western blotting method.

MTT法檢測單獨應(yīng)用阿霉素或阿霉素與皂角皂苷聯(lián)合作用的SMMC-7721細胞的生存率；RT-PCR檢測bcl-2 mRNA表達久久久一本高清免费；Western blotting檢測bcl-2蛋白的表達亚洲欧美日韩国产另类。

Objective To evaluate the effects of glomerular cell proliferation and apoptosis in experimental glomerulosclerosis.

用RT-PCR方法和免疫組化法檢測增殖細胞核抗原的表達，原位檢測腎小球細胞凋亡指數(shù)黄色视频网址免费看。

Methods: hPDLCs of passage four were treated with 0.1% absolute ethyl alcohol, 10^(-10)mol/Lor 10^(-7)mol/L E, in DMEM supplemented with 10% FBS, 10mM β-glycerophosphate and 5μg/ml ascorbic acid for 24 hours, 48 hours and 72 hours.

采用劑量對照設(shè)計欧美日韩精品精品区，評價四代hPDLCs分別在含有0.1%無水乙醇人人上人人操人人干、10^(-10)mol/L E2或10^(-7)mol/L E2的礦化誘導(dǎo)培養(yǎng)基中培養(yǎng)至72h，時間點的觀察熒光定量RT-PCR檢測RANKL和OPC mRNA的表達水平毛片一区网站。