By the use of RACE PCR technique with RNA extracted from Lycoris radiata young leaves as template and primers specifically designed according to conservative sequences of Amaryllidaceae lectins, the full length cDNA of Lycoris radiata lectin was cloned.

用石蒜科植物凝集素基因的保守序列為引物好紧好深好大乳无码中文字幕，利用RACE PCR技術(shù)從石蒜幼嫩葉片中克隆出石蒜凝集素的全長cDNA 日韩图亚洲一区。

Using PCR and RACE technique, We obtain the cDNA and genomic DNA sequence of the lac1 gene from Auricularia polytrica.

本文利用PCR和RACE技術(shù)首次從毛木耳AP4菌株中獲得編碼漆酶基因的cDNA及其基因組全長序列免费欧美操屄，基因組大小為2514 bpav在线资源播放。

In this paper, the myogenic bHLH gene, AmphiMDF, expressed in the embryos of amphioxus (Branchiostoma belcheri tsingtaunese), has been cloned by the methods of degenerative PCR and RACE.

本文利用簡并性PCR和RACE等方法克隆了青島文昌魚(B.belcheritsingtaunese)胚胎發(fā)育過程中表達(dá)的生肌bHLH基因毛片无码全部免费，AmphiMDF。

In this study, 526 base pair DNA fragment of OMT were obtained from Chamaecyparis formosensis. Then the 5' and 3' RACE coupled with the Genome walking were used to clone the full length of CCoAOMT, named CfCCoAOMT. After combining the sequence data, there is a total of 1721 bps in full length including promoter sequence.

本研究於紅檜試材中在线无码第一区亚洲第一页，選殖出526個堿基對之Caffeoyl CoA 3-O-methyltransferase片段基因序列免费A级毛片在线A级，再經(jīng)由5'和3' RACE及Genome walking，共獲得計有1721個堿基對国产91视频福利，包含啟動子及基因全長91精品自在拍精选久久，命名為CfCCoAOMT精品久久久久久电影。

By using RACE method,3' flanking sequences of PPO gene was amplified from cDNA of young fruit of strawberry.

本文用RACE的方法，從草莓幼果cDNA中成功擴(kuò)增出PPO基因的3'端部分序列免费看的黄色视频。

Full-length cDNA of Gibel carp pou2 gene was cloned from gastrula SMART cDNA library through RACE-PCR. It consists of 2421 bp, with an ORF of 1416 hp encoding a protein of 471 aa. The complete amino acid sequence shares 91.0% identity with zebrafish pou2 gene product.

用RACE-PCR方法從原腸期SMART文庫中擴(kuò)增到銀鯽pou2基因的全長cDNA99riav1com，其全長為2421bp，開放閱讀框為1416bp日韩成年黄网站视频，編碼471個氨基酸欧美在线1区，與斑馬魚pou2基因的氨基酸序列一致性高達(dá)91.0%。

The resulting populations of proteins were considered as induced protein positive to heat stress. Using LC-ESI MS/MS, several proteins were identified, including glyoxalase I and 3beta-hydroxysteriod dehydrogenase. Degenerative primers were designed and RACE technique was applied to obtain the complete gene sequences of the two-mentioned proteins from heat-sensitive and heat-tolerant tomatoes.

經(jīng)由此研究最新国产91自拍，成功地找出多個具有高度差異性的蛋白質(zhì)超级碰免费，在功能分析及歸納整理之后，挑選了其中兩個蛋白質(zhì)glyoxalase I和3beta-hydroxysteriod dehydrogenase欧美不卡三级视屏，設(shè)計degenerative primer日本、乱论、欧美、国产，并利用RACE技術(shù)將上述2種蛋白質(zhì)在二種番茄品系中的完整基因序列定出。

The key research progresses and results that we accessed by the project were as follows: 1 A pair of degenerated primers were designed in the conserved domain which based on the amino acid sequence of HAK in Arabidopsis thaliana. The 500 bp fragment of HAK were amplified from the roots of Puccinellia tenuiflora by RT-PCR. The full sequence of HAK were obtained by 5' and 3' RACE methods and were determined and analyzed.

本項目主要取得了以下研究進(jìn)展和成果：（1）根據(jù)已有HAK轉(zhuǎn)運(yùn)蛋白氨基酸序列在保守區(qū)域設(shè)計簡并PCR引物强奸乱伦日韩，用反轉(zhuǎn)錄PCR方法国产一二在线，擴(kuò)增出500bp的小花堿茅根HAK片段，通過5''和3''RACE的方法獲取了該HAK的全長序列92国产视频，完成了測序和序列同源性分析www.亚洲视频黄色免费。

Therefore, the LsHNXs genes were cloned in this study, and their function was studied by RNAi. The main results were shown as follows:1 The conservative sequences of three LsHNXs genes (LsHNX1, LsHNX2, and LsHNX3), and the 3'-terminal specific sequences of LsHNX2 and LsHNX3, were cloned by RT-PCR and 3'-RACE.2 The plant RNAi vectors were constructed and the transformation of Limonium sinense was done. The RNAi lines of three single-genes and one double-gene were obtained and were propogated rapidly for the molecular and the stress tolerance analysis.3 To be compared with wild type, the Na+ and K+ contents secreted by the leaves of LsNHX1 transgenic plants were remarkably higher.

主要實驗結(jié)果如下：1采用RT-PCR和3'-RACE方法從補(bǔ)血草中克隆獲得LsNHXs基因家族三個成員LsNHX1、LsNHX2和LsNHX3的保守區(qū)序列以及LsNHX2和LsNHX3的3'端特異序列欧美性爱一级黄。2構(gòu)建植物基因RNAi沉默表達(dá)載體并轉(zhuǎn)化補(bǔ)血草欧美性爱在线视频一区二区，獲得三個單基因和一個雙基因的RNAi沉默表達(dá)植株，快繁轉(zhuǎn)基因植株供分子鑒定和耐逆分析男人的天堂无码视频。3LsNHX1轉(zhuǎn)基因植株葉片表面分泌物中的Na+亚洲一级视频、K+離子含量比野生型對照顯著增加；葉組織內(nèi)Na+離子含量和K+離子含量均略高欧洲黄色三级片高清无码，但差異不顯著91老女露脸在线观看；K+/Na+比沒有顯著差異。4LsNHX2轉(zhuǎn)基因植株葉片表面分泌物中的Na+離子含量比野生型對照有所下降欧美日韩中文字幕在线不卡电影，但差異不顯著变态一区；K+離子含量比對照顯著降低。

In contrast,the expression levels of Lb est2 in nymphal stages are lower than that in adult stage.2 Gene cloning of AChE and mRNA expression level in L.entomophila2.1 Gene cloning and sequence analysis of AChE in L.entomophilaTwo full length cDNA encoding AChE were cloned from L.entomophila by the methods of RT-PCR and RACE,named Le ace1(GenBank Accession No.:EU854149) and Le ace2GenBank Accession No.

CarE基因在不同發(fā)育階段mRNA表達(dá)量的分析結(jié)果表明手机在线一级电影，Lb est1的表達(dá)量隨著該蟲生長發(fā)育的進(jìn)行逐漸降低色97综合视频在线观看视频，至成蟲期時表達(dá)水平最低；相反A级全黄片一级全黄片，L6 est2在若蟲期表達(dá)水平較低8050午夜一级少妇影院大陆，而在成蟲期表達(dá)水平最高。2嗜蟲書虱乙酰膽堿酯酶基因克隆及其mRNA表達(dá)水平研究2.1嗜蟲書虱乙酰膽堿酯酶基因克隆及序列分析利用RT-PCR和RACE技術(shù)成功克隆獲得嗜蟲書虱2個AChE基在的全長序列91在线欧美超碰，分別命名為Le ace1(GenBank登錄號：EU854149)和Le ace2(GenBank登錄號：EU854150)婷婷综合五月天丁香激情。