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We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

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This study was conducted in Shao-ya diaklings,a laying type duck,to determine its abilities of meat production and feed protein utilization before 12 weeks of age.In the grouped feeding trial and nitrogenbalance test,five isoenergetic diets(2950 kcal calculated ME/kg as fed)were used.The crude proteinquality was essentially the same,and their contents were 27.5,23.0,18.4,15.2,and 12.0,respectively.The results were as follows.The growth rate of draklings increased almost linearly during the first 3 we...

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By taking advantage of the abilities of grid, which can integrate the computing resources, storage resources, information resources and knowledge resources, Traffic Information System is effectively realized in the distributed, isomeric and data-varyi

基于網(wǎng)格的交通信息系統(tǒng)利用了網(wǎng)格這種融合計(jì)算資源黄片69、存儲(chǔ)資源国产欧美亚洲另类第三十页、信息資源、知識(shí)資源的能力91夜色精品国产网站，在分布波多野结衣操逼视频、異構(gòu)、數(shù)據(jù)多樣化的交通系統(tǒng)中得以高效實(shí)現(xiàn)天天日天天操天天日。

In complexes 8～16 synthesized by hydrothermal method, most of the ligands adopt multidentate coordination modes joining several lanthanide ions into high-dimensional coordination polymers. And changing of 5-substituting group on isophthalic acid has a little effect on the coordinated abilities of the meta-carboxyl groups.

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Humans are born with the Jacobson's organ, but in early development its abilities dwindle to a point that it is useless.

人類天生就有梨鼻器亚洲区欧美性爱，但是在早期發(fā)展中日韩性网站，它的功能退化到可有可無的地步。

The extraction and purification conditions of flavonoids from leaves of Jasminum nudiflorum Lindl. and their abilities of eliminating free radicals were researched.

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Kotoko is not very smart, having been in Class F for all through high school (students are sorted by abilities and Class F is the lowest) and being in the department in the university considered as Class F.

琴子不是很聰明爱搞网站无遮挡，在整個(gè)高中時(shí)代都在F班（學(xué)生們按照成績分級(jí)小黄片A片，F(xiàn)班是最差的一個(gè)班），而且她上的大學(xué)也和她所在的F班一樣爛欧美一级作爱片。

Yet for all her attitude and her mind-reading abilities, Kristi has a vulnerable side.

然而對(duì)于她的這種態(tài)度以及她擁有的讀心的能力日本亚洲国产精品麻豆www，克里斯蒂也有一個(gè)弱點(diǎn)。

As to'Cabemet Sauvignon'and'Queen of Vineyard', which belong to the'West Europe Cultivars Group', no obvious decline of Fv/Fm was found in their potted plants even under severe drought stress. Their photosynthetic abilities also greatly recovered after rewatering, i. e. no serious photodamage was formed in these cultivars under drought stress. Between them, the φPSⅡ of 'Queen of Vineyard'under severe drought stress was much lower than that of'Cabernet Sauvignon', which suggested that the light use efficiency of the former was much lower than the latter under severe drought stress. The potted plants of'Autumn Royal'and'Rizamat' sustained high Fv/Fm and φPSⅡ before moderate drought stress, but seriously declined under severe drought stress, which indicated that these cultivars had a weak resistant ability to extreme drought stress. A hybridized cultivar of Vitis vinifera L. and V. labrusca L.,'Red Double Taste', its Fv/Fm and φPSⅡ expressed mild decline along drought stress grade.φPSⅡ of'Red Double Taste'was the highest among seven cultivars under severe drought stress condition, which might closely connected with its high photorespiration activity. Another new cultivar,'Red Globe', and a rootstock,'1103Paulsen', the Fv/Fm and φPSⅡ of their potted plants declined markedly under slight drought stress, which suggested that they held a weak ability to defend against photoinhibition.

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