Studies on callus induction, adventitious shoot regeneration of Cirsium japonicum DC. var. takaoense Kitamura using leaf and stem-node as explants were conducted with different strength of MS medium, sucrose concentration and growth regulator combination in this investigation.

中文摘要本研究以白花小薊無(wú)菌苗之葉片與莖節(jié)培植體為材料，進(jìn)行不同MS培養(yǎng)基濃度激情视频在线观看、蔗糖濃度国产精品va在线观看一、NAA配合BA或TDZ濃度組合對(duì)愈合組織形成及不定芽誘導(dǎo)之影響。

Cotyledonary node explants were prepared from 5-7 day old seedlings, infected and co-cultivated with Agrobacterium tumefaciens. Resistant adventitious buds emerged after 4 weeks on selective medium.

從發(fā)芽5-7天的大豆無(wú)菌苗切取子葉節(jié)外植體国产在线观看黄A片免费网站，經(jīng)農(nóng)桿菌感染和共培養(yǎng)后2018偷拍视频，在選擇培養(yǎng)基上4周左右出現(xiàn)抗性不定芽。

Diospyros kaki Thunb cv.Fuyu and Jiro are Japanese persimmon varieties. Dormant buds taken from persimmon trees, as in vitro explants materials, were set up successfully. New shoots were attained. Callus was induced and adventitious buds were differentiated from leaves and roots of tissue cultures; new shoots propagation and shoots rooting were studied in different basal medium added different hormones.

本研究以甜柿品種富有柿與次郎柿為材料欧美一级免费，系統(tǒng)地研究了兩品種的組培快繁欧美野外打野战A片视频、組培苗葉片和根段的不定芽再生、愈傷組織的誘導(dǎo)和不定芽的分化黄色网站高潮视频免费观看、不定根的誘導(dǎo)等技術(shù)玖玖玖无码视频，以期為柿果的轉(zhuǎn)基因技術(shù)奠定基礎(chǔ)，同時(shí)也為富有柿與次郎柿苗木的快速繁殖提供技術(shù)依據(jù)美女做视频在线播放网站。

In this research, we have studied the effects of a series of factors (genotypes, basicmedia, plant growth regulators, explants, inoculation methods, leaf ages and darkperiod) in the inducing adventitious shoot of strawberry(Fragaria ananassa Duchcv.

本試驗(yàn)以草莓品種&豐香&和&章姬&為試材日本黄色视频日本黄色视频，研究了草莓離體再生過(guò)程中基因型、基本培養(yǎng)基欧美一级免费看黄片视频、植物生長(zhǎng)調(diào)節(jié)劑配比欧美性爱23TB、不同外植體、接種方式av在线www,、苗齡和暗處理對(duì)草莓離體再生不定芽的影響一a级视频，獲得了草莓高效的離體再生體系。

The cotyledon explants excised from 5-day-old germfree seedlings were cultured on the MS medium containing 1.0 mg/L 6-BA in darkness for 3 days, and then further cultured under light condition for induction of adventitious buds.

結(jié)果顯示：將從5 d齡甜瓜無(wú)菌苗上切取的子葉外植體接種在MS+6-BA1 mg/L的不定芽誘導(dǎo)培養(yǎng)基上人人干人人玩人人摸，黑暗條件培養(yǎng)3 dA片免费视频网站入口，再轉(zhuǎn)至光下繼續(xù)培養(yǎng)，誘導(dǎo)出高效不定芽叢亚洲一级av高清毛片。

If the ratio or the concentration of 2, 4-D was higher, the callus formed quickly, but it was hygrophanous, and easy to necrosis, conversely, if the ratio or the concentration of 2, 4-D was lower, the callus appeared very slowly or could not form, and adventitious root formed directly or indirectly.

比值較高（或2黄色免费观看网站网址，4-D濃度較高）時(shí)愈傷組織出現(xiàn)的時(shí)間較早，但愈傷色澤不好800av凹凸在线观看，呈半透明水漬狀女18毛片亚洲一级毛片，易于褐化死亡，而比值較低時(shí)日本强伦奸人妻一区二区，愈傷出現(xiàn)得很慢或不形成愈傷玖玖国产精品视频，且易于直接或再分化形成不定根；這些不定根長(zhǎng)至1.5-2.0cm時(shí)就不生長(zhǎng)印度AV在线网站，并逐漸褐化黄页在线免费观看色秀。

Adventitious shoots were successfully regenerated from young leaves and internodal segments of in vitro cultures of two chrysanthemum cultivars when cultured on Murashine and Skoog basal medium supplemented with various plant growth-regulators.

以MS為基本培養(yǎng)基添加不同質(zhì)量分?jǐn)?shù)的6-BA和NAA欧美在线视频首页，通過(guò)一定時(shí)間誘導(dǎo)，幼嫩葉片和莖段可以通過(guò)不同方式產(chǎn)生不定芽永久在线精品免费播放。

The dissertation studies on the conflict and minglement between the Chinese theory of Literature and Art and the tendency of adventitious theory of Literature and Art, in the tide of globalization from the latter half of twentieth century.

本論文研究20世紀(jì)以來(lái)一级a作爱在线视频观看，中國(guó)文論在全球化浪潮中，與外來(lái)文論趨向的沖突欧美女肏屄、交融日韩一级A片在线高清视频，以及在此過(guò)程中如何重建中國(guó)文論的問(wèn)題。

After 5～7d of culture,adventitious buds appeared directly from explants without any intervening callus on Murashige and Skoog medium supplemented with different concentrations of N-6-benzyladenine alone or together with 0.5mg/L α-naphthaleneacetic acid.

在附加不同濃度N-6-benzyladenine(6-BA)或與低濃度α-naphthaleneacetic acid組合的MS培養(yǎng)基上又爽又刺激的视频网站，5~7d外植體表面不經(jīng)過(guò)愈傷組織誘導(dǎo)階段美女任人插黄色视频，直接形成不定芽。

When the protoplast-derived calli were transferred into the MS solid medium added with 2.0mg/L benzylamino-purine and 0.4mg/L naphthaleneacetic acid and cultivated in the codition of low illumination, after which the adventitious shoots were induced, then we could obtain numerous plantlets and could transplant them survival to pots in the greenhouse.

當(dāng)將原生質(zhì)體分裂形成的愈傷組織轉(zhuǎn)移到附加2.0mg/L6-芐氨基嘌呤和0.4mg/L萘乙酸的MS固體培養(yǎng)基上免费的看黄网站，并在低光照條件下培養(yǎng)后欧美日韩人妻自拍，從愈傷組織上分化出了不定芽，進(jìn)而發(fā)展成小植株最近高清在线视频观看，并移栽成活4438最新全国免费视频网。