We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

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Obviously, the optimization management in middle-sized or small cities of China is very necessary. The basic characteristics of municipal solid waste generated in middle-sized or small cities of China are put forward in the paper. The total amount of municipal solid waste generated in middle-sized or small cities is significantly less than that in large cities. The amount of garbage is about 1 kg per capita and year. In the garbage, inorganic material is more than organic material, the combustible less than incombustible and the amount of uncompostable garbage more than the amount of garbage that can be disposed for composting. The value of heat of garbage is about 800 kal/kg.

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Through the photographic technology of fisheye lens and achieved the space image of cotton canopy structure distributing at different condition, and setupped cotton canopy structure base after image analysis. The experiment results indicated that the adaptability of different varieties to plant density is different, reasonable plant density is benefit to the raise of varieties product, and Xinluzao10 is better at high density（225000plant/ hm2） condition and Xinluzao12 is better at mid-high density（150000 plant / hm2） condition. For different nitrogen, Xinluzao10 is better at right nitrogen application amount（600 kg/ hm2） condition and Xinluzao12 is better at mid-high amount（900kg/ hm2）condition. For different irrigation, Xinluzao6 and Xinluzao12 is better at limited drip irrigation amount（1500 m3/ hm2） condition and Xinluzao8 Xinluzao13 is better at right drip irrigation amount（3000 m3/hm2） condition.

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The soil erosion process on the slope was clearly divided into sheet erosion stage, rill erosion developing stage and rill erosion stable stage. Before lOminutes of rainfalls, the percentage of sheet erosion amount to total erosion amount was more than 90%, and it indicated that sheet erosion happened on the slope. This percentage sharply decreased before SOminutes of rainfall time, on the other hand, the percentage of rill erosion amount to total erosion amount increased in curve form with rainfall time.

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The amount of different mineral elements in different parts of male and famale B. thoracica had big difference, except Zn; the amount of other mineral elements in female breast muscle were all higher than that in male breast muscle, and the amount of Ca, Fe in leg muscle, Ca, Cu, Mn in skeleton and Cu, Mn in feathers of male were all higher than that of female, but the amount of Ca, Fe, Zn in feathers of female were higher than that of male.

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On the base of probabilistic and statistics results of joints and cracks, the Author modifies Block Theory. The sliding probability of Key Block is I A RSTR A CT F2 ? 5.It is put up forward that GM(l,1) Model to predict Amount of rainfall, and Markov Chain to modifY GM( 1,1 )predicting result, and then a theoretical predicting Model of amount of rainfall is firstly built. This model is applied in a situ example. It is proved that the model is very useful to predict amount of rainfall ,when ordinal array of Amount of rainfall changes in unexact index rule.

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