Methods Amplify the cDNA sequence encoding truncated HCV gene, with a part of carboxyl-terminus deleted, by PCR. Synthesize the mimic epitope at E2 region of HCV and seven T or Th cell epitope genes of NS3-NS5 respectively. Bind HCV core gene with a part of carboxyl-terminus deleted to the synthetic epitope gene by PCR, then clone into eukaryotic expression vector pcDNA3.1 and transiently transfect COS7 cells.

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The virus was purified by ultracentrifuge. According to the NS gene sequence published by Genbank, One primer T-1 was designed to amplify the cDNA by reverse transcript.

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To build the service government, we should uprear new values of government management 超碰超碰超碰9在线巨乳、strength basic public service functions of government and amplify responding mechanism of government.

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Fourteen wampee cultivars collected from Guangdong and Guangxi were used for RAPD analysis in this study. 16 primers selected from 150 random primers were used to amplify 87 DNA fragments and 57 bands (54.0%) showed polymorphisms.

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Using a pair of special primers to amplify the exon 3 of Myostatin gene in Northeast Wapiti, we aimed to detect the polymorphisms of Myostatin gene.

應用pcr-sscp和測序的方法對東北馬鹿Myostatin基因的第3外顯子進行了多態(tài)性分析麻豆AV在线网站。

According to 5'flank regions and partial sequences of the MyoG gene in Sus scrofa, Bos taurus, Homo sapiens, and Mus musculus published in GenBank, the homogeneous primers were designed to amplify the promoter region of Gansu wapiti MyoG by Touch-Down PCR.

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You can cut, delete, fade in/out, copy, amplify and normalize the recorded waveform with a built-in audio editor and save it into MP3 and WAV format.

你可以剪切r日韩一级黄色网站，刪除，淡入/淡出一级做a爱过程免费视频韩国，復制黄片69，放大和規(guī)范其保存到記錄波形與一個內(nèi)置的音頻編輯器和MP3和WAV格式。

Clipping and DC offset detection and reduction helps you to enhance recording quality with any audio recording device. You can cut, delete, fade in/out, copy, amplify and normalize the recorded waveform with a built-in audio editor and save it into MP3 and WAV format.

Steady Recorder還帶有一個內(nèi)建的簡單音頻編輯器日韩手机在线视频，可以進行剪切91夜色精品国产网站、刪除，淡入淡出精品麻豆国产免费一区二区三区、復制国产精品一级毛片www，放大或正常化波形等操作95色色色，并可以將錄制后的音頻保存為MP3或WAV格式亚洲色一区，支持MP3 VBR。

With AudioEdit Deluxe you can perform two-way conversions among CD, MP3, MP2, WAV, WMA, and OGG; open, create, and save audio files in any of the supported formats (can also save any portion of a loaded file to disk as a new file); display audio data waveform (Zoom Full, Zoom In, Zoom Out, Zoom Vertical); play audio files or any portion of the files; record audio data from a microphone or any other available input device; edit audio files visually (Cut, Copy, Delete Selection, Crop, Trim, Paste, Paste From File, Mix, Mix From File); apply various effects (Amplify, Compressor, Delay, Equalize, Fade In and Fade Out, Flanger, Invert, Normalize, Phaser, Reverb, Reverse, Shrink, Silence, Stretch, Vibrato); apply different filters to any selected portion of audio files (Band Pass Filter, High Pass Filter, High Shelf Filter, Low Pass Filter, Low Shelf Filter, Notch Filter); insert noise or silence into audio files; Insert information into audio files (Album, Artist, Copyright, Genre, Title, Year); complete, multi-level undo and redo capabilities; advanced, burn-proof audio CD recording; and much more.

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Open, create, and save audio files in any of the supported formats (can also save any portion of a loaded file to disk as a new file); Display audio data waveform (Zoom Full, Zoom In, Zoom Out, Zoom Vertical); Play audio files or any portion of the files; Record audio data from a microphone or any other available input device; Edit audio files visually (Cut, Copy, Delete Selection, Delete Silence, Paste, Paste From File, Mix, Mix From File); Apply various effects (Amplify, Compressor, Delay, Equalize, Fade In and Fade Out, Flanger, Invert, Normalize, Phaser, Reverb, Reverse, Silence, Shrink, Stretch, Vibrato, etc.); Apply different filters to any selected portion of audio files; Supports all major audio file formats such as uncompressed WAV; compressed WAV; MP3, MP2; Ogg; WMA; CDA; AVI; AIFF; AU; G.721, G.723, G.726; VOX; RAW.

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