Results By optimization test, capture antibody and detection antibody (from PVA detection kit, Agdia, USA) were finally diluted at 1:50 (4ug/mL) and 1:25 to be used in one step CBA, respectively. A total CBA reaction time of 2 h was needed. No cross reactivity with other plant virus similar to potato virus Y, lettuce mosaic virus and tomato ring spot virus were found. Detection sensitivity for positive control sample in one-step CBA was 4 folds higher than that in normal plate double antibodies sandwich enzyme-linked immunosorbent assay.

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There were no difference in sera thyroglobulin antibody and thyroid microsomal antibody levels, age, sex and course in two groups.

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Objective: To establish the immunoassay of thyroglobulin and anti -Tg antibody, with use of Tg antiserum and Tg monoclonal antibody by immunization of highly purified Tg.

目的：提取高純度的甲狀腺球蛋白，為制備 Tg 抗血清及 Tg 單克隆抗體日韩成人教师在线播放视频，建立 Tg 的檢驗(yàn)方法及 TgAb 的測(cè)定方法做準(zhǔn)備。

Anti-endometria antibody IgG react with uterus internal membrane disturbing the embedding and growth of the fertilized egg. The tilter of the anti-endometria antibody IgG has relationship with the early habitual abortion.

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We immunized the rabbit and have got high titter antibody. The antibody showed specifity with Western blot.

通過(guò)SDS-PAGE切膠回收獲得了高純度的表達(dá)產(chǎn)物久久热免费视频，進(jìn)而免疫家兔獲得了相應(yīng)抗體。

Coli genome was amplified by using PCR method. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the Mn-SOD protein expressed by pET-28a vector and purified was injected into rabbits to prepare polyclonal antibody. Western-blot was used to detect the effect of polyclonal antibody.

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The same result can also be achieved when optimizing F8 function (Rastrigin's function). Comparing to EGA, KEGA is easy to realize and the increase of computation load can be neglected, but the performance of KEGA can be improved greatly. 2. To enhance the speed of AIA, two new methods of computing the concentration of antibody were developed. Simulation results for F8 and F15 (needle in haystack: type I) functions show that the two methods can improve the running speed of AIA more than 8. 5 times. 3. As to the drawback in defining antibody concentration, a new definition based on Euclidean distance between two antibodies and fitness is proposed. On this basis, a new AIA was constructed. The convergence performance are more better than that of AIA. 4. Combining king crossover with AAIA and DBAIA, two new AIAs, KAAIA and DKBAIA, were proposed.

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The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

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Diagnostic performances of anticyclic citrullinated peptides antibody and antifilaggrin antibody in Korean patients with rheumatoid arthritis.

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The optimum pH for labeling was between 7.5-8.0, and the optimum spiking rate of polyclonal antibody was 12μg/ml. The absorb pad, sample pad, glass fiber of ARIST and nitrocellulose membrane HF24004 of Millipore were chosen as GICA materials. The carbofuran-OVA conjugate 0.97mg/ml and sheep anti-rabbit IgG 0.48 mg/ml was dispensed with Nano-Plotter (NP 1.2) onto the NC membrane to serve as test line and control line, respectively. The colloidal gold-labeled anti-carbofuran antibody was dispensed on the glass fiber with 20μl/unit.

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