Weigh on the 1 st day of the 8th week, postanesthetic, the broken marrow is put to death , take out the heart , liver , brain , kidney and musculus quadriceps femoris and put and clean the blood in icing the physiological saline immediately , blot with the filter paper, weigh.

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If my step hath turned out of the way, and mine heart walked after mine eyes, and if any blot hath cleaved to mine hands

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If my step hath turned out of the way, and mine heart walked after mine eyes, and if any blot hath cleaved to mine hand

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By using electroporation transfects,the TGF〓expression vector were introduced into HL-60 cells.Thecells were allowed to grow for 1 day before beingsubjected to selection for the ability to grow in mediumcontaining 600ug/ml geneticin.Stable cell clones resistantto G418 sulfate were obtained after 2-3 WK growth withdrug-containing medium.Southern blot hybridization wascarried out.RNA slot have higher expression of TGF〓mRNA in HL-60 transfected.All results followed suggestedthat TGF〓 have suppressed the maliganant phenotype of HL-60 cells.

利用電擊法將〓表達(dá)載體導(dǎo)入HL-60細(xì)胞中，經(jīng)G418篩選培養(yǎng)20天后再行Southern雜交檢測外源〓整合情況欧美特一级AAAAA禁片，利用RNA狹線雜交法證明了在轉(zhuǎn)錄水平上表達(dá)有所提高国产一级特级黄色网站，進(jìn)一步進(jìn)行了流式細(xì)胞儀測定DNA含量，使細(xì)胞在〓期產(chǎn)生生長阻滯作用黄色网站aaaaawwwwwwww，細(xì)胞生長速度99九九免费精品，在軟瓊脂中成集落數(shù)、在組織中的浸潤性及在裸鼠中的成瘤性均發(fā)生變化综合精品21p，我們的實驗結(jié)果表明TGF〓對HL-60細(xì)胞具有抑制惡性表型的作用中文亚洲日韩特黄无码免费看电影。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

設(shè)計一對引物從gE全基因克隆載體pgE中調(diào)出抗原決定簇密集編碼區(qū)，克隆進(jìn)PET-32a表達(dá)載體无码AV动图天堂18国产，雙酶切和PCR鑒定出陽性者图片乱伦网站，命名為PET-32pgE，轉(zhuǎn)化BL21菌株九九C片一级黄片，挑取單菌落在加有氨芐的2×YT培養(yǎng)基中37℃過夜長至飽和永久黄网播放站色视频免费，按2％體積轉(zhuǎn)種另一管，培養(yǎng)至OD_(600)為0.6丝袜人妻无码中文字幕综合网，加IPTG至終濃度1mmol／l，誘導(dǎo)4～6小時色色色色香蕉，SDS-PAGE和Westernblot分析欧美一区二区中出视频，證明確實表達(dá)并具有免疫反應(yīng)性午夜AV。