TheMADS box locates just at the 5'end of most of genes and is highly conserved.According to these,a primer was designed and the effort to isolate new membersof MADS box gene family in rice genome by RT-PCR was made.

絕大多數(shù)已克隆到的這類基因的MADS-box恰好位于5'端羞羞真人视频，而且非常保守欧美性爱视频一日本高清视频，因此国内黄色a片不卡，我們根據(jù)MADS box基因的保守區(qū)設(shè)計了一個特異的引物亚洲精品二区中文字幕，試圖通過RT-PCR分離出水稻花中MADS box基因家族的新成員A片β片毛片免费视频。

Further bioinformatical analysis indicated that the three Y-box genes of lamprey, i.e., Lyb1, Lyb2, and Lyb3, encoded 331, 181, and 171 amino acids, respectively. The three amino acid sequences were homologically aligned with those from Ciona intestinalis, Danio rerio, Oryzias latipes, Takifugu rubripes, and Mus musculus by DNAMAN software.

采用DNAMAN軟件，將七鰓鰻的3個Y-box蛋白氨基酸序列與海鞘操一操在线、斑馬魚91视讯亚洲一二三区、清鳉、河豚魚和小鼠Y-box蛋白進(jìn)行同源性分析免费AV黄片，結(jié)果顯示他們共有一個保守的冷休克結(jié)構(gòu)域强奸a片久久a，其中包含兩個RNA結(jié)合基序RNP-1和RNP-2，表明獲得的Lyb基因?qū)儆赮-box 基因家族成員一区无码在线。

CAAT box" and "TATA box, potential regulatory motifs from transcription, and conserved cis-acting regulator elements were discovered within LTRs in the great number of sequences using plantCARE sortware.6 The insertional polymorphism of LTR-10 element within the genome of 8 wild species and 28 cultivars of Mallus Mill. was studied by sequence-specific amplification polymorphism.

分析獲得的Tyl-copia類逆轉(zhuǎn)座子的LTRs未满十八18禁止观看网站，其中含有啟動子的結(jié)構(gòu)特征"CAAT box"和"TATA box"及受不同脅迫條件作用的調(diào)控元件。6影音先锋操逼网、基于蘋果Tyl-copia類逆轉(zhuǎn)座子LTR-10的SSAP技術(shù)在蘋果屬8個野生種和28個栽培品種中表現(xiàn)出了豐富的遺傳多態(tài)性my国产精品免费，多態(tài)性片段比例為88.2％。

So it is assumed that the E-box might be associated with the enhancement of transcription activity.Here, the LTRs of WH17 ,DLA-25,DLA-118 and LTR with single mutation in the E-box motif were separately cloned into pCAT-Basic vector resulting in a series of recombinant plasmids containing CAT reporter under the control of different versions of LTRs.

通過對EIAV分離株WH17的LTR與L株99se在线播放、DA和DLA株的LTR序列進(jìn)行比較天天婬色婬香综合网站日本，發(fā)現(xiàn)其在在U3-R結(jié)合處多一個E-box基序，推測該基序的變化可能會起到促進(jìn)轉(zhuǎn)錄作用亚洲日韩欧美另类，為此將EIAV強毒株(DLA-25)色综合无码视频、驢白細(xì)胞弱毒株(DLA-118)毛片A片在线、EIAV分離株WH17以及U3-R結(jié)合處的E-box基序點突變的LTR分別克隆到pCAT3-basic質(zhì)粒中的報告基因氯霉素乙酰轉(zhuǎn)移酶的上游，獲得一系列受不同LTR控制的CAT表達(dá)質(zhì)粒国产三级综合。

The ubiquitin proteolytic system is an important selective proteolytic system of the celler proteolytic systems, a number of them were identified as a ubiquitin chain assembly factor-E4. The U-box protein is a new family ubiquitin-protein ligase(E3), which determine the substrate specificity of ubiquitination. The U-box is a domain of 70 amino acids that is highly conserved among yeast, plant and animals, but more U-box proteins were found in plants than in animals.

泛素系統(tǒng)是選擇性降解細(xì)胞內(nèi)蛋白質(zhì)的重要系統(tǒng)之一国产三级精选国产麻豆，U-box蛋白質(zhì)是此系統(tǒng)中決定底物特異性識別的一種新型E3蛋白質(zhì)，部分U-box蛋白質(zhì)屬于泛素鏈聚集因子-E4.U-box結(jié)構(gòu)域大約由70個氨基酸殘基構(gòu)成91国在线国内播放女神学生，在酵母5060网午夜一级毛片免费视频、植物和動物等真核生物中保守存在，但植物中的數(shù)目遠(yuǎn)多于動物中在线视频99re。

At a glance of the molecular mechanism, one gene encoding 218 amino acids was identified from cDNA-AFLP differential study and then named as Ipomoea batatas MADS-box 1 gene (IbMADS1) based on earlier studies. From sequence alignment and phylogenic analysis, IbMADS1 exhibited a typical type II-MIKC structure, including two conserve domains, MADS-box and K-box.

本論文針對從甘薯塊根篩選出的MADS-box基因高清无码性爱片，IbMADS1（Ipomoea batatas MADS-box gene 1）進(jìn)行分析，分別探討該基因在甘薯各部位的表現(xiàn)方式日韩18禁图片区综合、受植物荷爾蒙調(diào)節(jié)的影響www.jizz.com中国破处，以及甘薯屬中野生親緣種間的基因表現(xiàn)歧異性，再進(jìn)一步從轉(zhuǎn)殖植物的外表形態(tài)了解此基因可能的生理功能日韩精品娱乐在线观看网站免费。

The analyses indicated that the upstream sequence contained several regulatory elements,such as CCAAT-binding factor,GC-box,HSE and GATA,but TATA-box and CAAT-box were not be found.

分析表明一级做a爱过程免费视频韩国，該上游序列的轉(zhuǎn)錄起始位點位于第3255位G堿基處，沒有明顯的TATA-box和CAAT-box国产欧美亚洲另类第三十页，但含有CCAAT-binding factor日韩手机在线视频、GC-box等重要的轉(zhuǎn)錄因子結(jié)合位點，以及熱激應(yīng)答元件HSE和GATA元件等啟動子順式調(diào)控元件国产激情视频久久久。

The Lyb genes were novel members of Y-box gene family containing a conserved cold-shock domain with two RNA binding motifs (RNP-1 and RNP-2). Moreover, phylogenetic analysis revealed that there was only one type of Y-box gene before the emergence of gnathostomata. By contrast, the Y-box gene were divided into three groups, YB1, YB2, and CSDA during evolution of jawed vertebrates.

此外精品麻豆国产免费一区二区三区， 從Swiss-Prot下載了經(jīng)實驗驗證過的Y-box蛋白序列數(shù)據(jù)，結(jié)合實驗克隆得到的Lyb基因所編碼的氨基酸序列構(gòu)建系統(tǒng)發(fā)育樹国产爆乳日韩精品视频迅雷下载，發(fā)現(xiàn)Y-box基因在有頜類出現(xiàn)以前只有一種類型95色色色，有頜類出現(xiàn)以后， Y-box基因分化成YB1AAA搡老熟女国产、YB2和CSDA三種類型首页国内自拍偷拍视频。

One of these clones,M79 was selected for further study.M79encodes a typical MADS box protein,and it shares high homology with many ofthe MADS box genes in plants.

對其中一個克隆M79的DNA序列和蛋白質(zhì)序列分析表明，M79基因編碼多肽含有一個典型的MADS box和K-box国产激情自拍小视频，與植物中的許多MADS box基因產(chǎn)物有很高的同源性99玖玖。

Polymorphism of HLA-DQB1 promoter region in Hans IDDM patients and normal controls have been identified by PCR, PCR/SSCP and PCR/sequencing methods.No differences were found in y and s box between patients and controls carrying different allele as well as in different ethnic groups. There are two different sequences in x box,but CCTAGAGACAGATT sequence locates frequently on the haplotype with DQB1.0302 allele. Polymorphism between transcription point and y box (at position －44～-46 and －59～-61) might be associated with the genetic susceptibility to IDDM. Additionally,a new single base mutant (CACC→CAC A ) was found at position －131 and －128 in two patients carrying DQB1.0601 allele.

結(jié)果顯示攜帶不同等位基因的患者與對照者DQB1 5'-調(diào)控區(qū)y、s box核苷酸序列相同国产在线b，且與白種人基因結(jié)構(gòu)一致国际免费黄色视频不卡；y box核苷酸序列存在二種結(jié)構(gòu)，CCTAGAGACAGATT序列常常與DQB1.0302等位基因在同一單倍型小黄片A片；轉(zhuǎn)錄起始位點至y box間-44至-61位存在多態(tài)性，-59至-61位AAG等位基因可能與1-型糖尿病易感相關(guān)聯(lián)欧美一级作爱片；在2例攜帶DQB1.0601等位基因患者的-131至-128位間發(fā)現(xiàn)CACC→ACA A單個堿基取代突變日本亚洲国产精品麻豆www。