In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

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This has the the advantage of keeping the shell history of the GUD buffer as well as GDB's breakpoints. You need to check, however, that the breakpoints in the recently edited code are where you want them.

不關閉GUD可以保留GUD buffer的shell歷史和GDB設的斷點，但是要檢查斷點是否還在原來位置夜夜草AV。

METHODS: Total RNA and protein were extracted by Trizol and RIPA Buffer from heart, brain, kidney, skeletal muscle and spleen tissues of C57BL/6 and BALB/c mice.

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We want our program to be windowed, and set the back buffer to a format

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Pointer to a buffer that receives value data.

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The first reaction mixture consisted of 5uL 10×PCR buffer,0.5uL 20mmol/L dNTP,1uL of primer F1AV网站免费不卡观看、R2(25umol/L),0.5uL Taq (2.5U),FTA filtering,42μL of double-distilled water,the whole system was 50μL;The reaction was run under the following conditions:DNA pre-denaturation at 94℃for 5 min;DNA denaturation at 94℃for 30 s,primer annealing at 58.8℃for 1min, extension at 72℃for 30s,for 25 cycles,and extension at 72℃for 5min.

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To investigate the methods to effectively and simply assess the CAG repeat size of HD gene which was necessary for gene diagnosis of Huntington disease, the sequence including polymorphic CAG repeat of HD gene was amplified by PCR with TaKaRa LA Taq DNA polymerase and GC buffer. PCR products were analyzed on polyacrylamide gel to distinguish normal alleles from HD alleles. The DNA fragments of affected alleles were recovered from polyacrylamide gel as templets for secondary PCR. The secondary PCR products were cloned into T vector for sequencing analysis to determine CAG repeat size. A total of 20 normal individuals and 3 members from a HD pedigree were included in this study.

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Here the term "ownership" does not infer "exclusive access"- and does not infer that you can modify the NDIS packet descriptor, buffer descriptor, VM or OOB data.

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NDIS packet descriptors, buffer descriptors, chained virtual memory and OOB data that you did not allocate yourself must be considered to be logically read-only.

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This is a common TCP problem in which a dropped segment with a large window causes the entire window's worth of messages to wait in a buffer (i.e., be blocked) until the dropped segment is retransmitted.

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