METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.

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The solutions were found to be good agrees with experimental results. The transfer characteristic of air dry/wet bulb temperature and spraying water temperature were discussed. The LMTD method based on this mathematical model is applicable to design and verify for the plate wet air cooler. The ratio of the wet bulb heat transfer coefficient to the heat transfer coefficient under air-cooled conditions was investigated theoretically and experimentally. The relative errors of the ratio between theoretical and experimental valves are small than 7 percent.

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Introducing the wet bulb specific heat capacity and the wet bulb heat transfer coefficient, a new mathematical model for the plate wet air cooler was developed based on the wet bulb temperature transfer characteristic.

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Recording electrode was placed on the scalp near the olfactory bulb. Results Electrically evoked olfactory potentials composed of triphasic negative-positive-negative peaks, named N1, P1, N2 respectively, were detected on the olfactory region. The latencies of N1, P1, N2 were 16.27ms, 25.36ms, 49.75ms respectively in cribriform plate.

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Energize the bulb at 14 Volts in the socket/circuit plate assembly as specified in Outgassing Test Conditions Table for 10 days.

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1100Mm. Top of bulwark to be angle or bulb profile, vertical plate with lower end flanged.

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Shake hands and hold pots, make humus tight, and then narcissus bulb plate located in the basin, and then continue to the soil about 1 cm, then Jiaotou water, and then pot in the shade , 5 days until bulb set to grow roots, it will flower pot into a sunny office culture ...

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The results show that the suitable material for treatment is the bulb basal plate tissue with a thin part of scales after having been inoculated for 7 days,and regeneration dose is 50～60Gy,the optimum treatment dose for breading is 15～20Gy.In the small bulbs treated with 15Gy and 20 Gy,two mutants were selected,one is shorter with more leaves,the other expands faster.

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In order to breed new germplasm for Narcissus tazetta,the bulb of Narcissus tazetta is used as explants.The adventitious buds is induced in MS media with hormone combination.γ-ray irradiation treatment of Narcissus tazetta combined with tissue culture is studied.Three materials,including the bulb basal plate tissues inoculated for 7 days,tissues transferred in fresh differentiation culture medium for three days after inoculated for 25 days,and small bulbs in vitro,are studied.

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