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Effects of dry temperature and dry time on properties of heat cured sand and the relationship with binder composition are as follows:sands bonded by binders that are not modified have high strength when dry temperature is high and dry time is long,but their anti-wettability power is poor . Sands bonded by binders that are modified by B but not modified by M can be dried quickly and sand strength is high but anti-wettability power is also poor. Sands bonded by binders that are modified by M but not modified by B can also be dried quickly to obtain maximum strength,then the strength becomes lower when they are dried continually for more time. and sand strength is high but anti-wettability power is also poor. Sands bonded by binders that are modified by M as well as by B at the same time can be dried quickly to obtain maximum strength at low temperature and short time. But the anti-wettability of sand mold is best when they are dried at high temperature and long time.

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With the advice and unanimous consent of our brothers, cardinals of the holy Roman church, by means of this our constitution which will have permanent validity, we establish, ordain, decree and define, by apostolic authority and the fulness of our power, that if it happens (which may God avert in his mercy and goodness towards all), after God has released us or our successors from the government of the universal church, that by the efforts of the enemy of the human race and following the urge of ambition or greed, the election of the Roman pontiff is made or effected by the person who is elected, or by one or several members of the college of cardinals, giving their votes in a manner that in any way involves simony being committed -- by the gift, promise or receipt of money, goods of any sort, castles, offices, benefices, promises or obligations -- by the person elected or by one or several other persons, in any manner or form whatsoever, even if the election resulted in a majority of two-thirds or in the unanimous choice of all the cardinals, or even in a spontaneous agreement on the part of all, without a scrutiny being made, then not only is this election or choice itself null, and does not bestow on the person elected or chosen in this fashion any right of either spiritual or temporal administration, but also there can be alleged and presented, against the person elected or chosen in this manner, by any one of the cardinals who has taken part in the election, the charge of simony, as a true and unquestionable heresy, so that the one elected is not regarded by anyone as the Roman pontiff.

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Therefore, depecage shall be adopted. The following rules are suggested: 1the existence, scope, duration and transferability of copyright and the unlawfulness in copyright infringement shall be governed by the law of the place where the works are effectively used. 2 Initial ownership of copyright works shall be governed by lex originis except works for fire, which should be governed by the laws that govern the employment contracts. 3 Contractual aspects of transfer of copyright through exploitation licenses or assignment should be governed by the laws chosen by the parties; in absence of choice, they shall be governed by the law of the country with which the contract is most closely connected (based on characteristic performance). 4 The legal consequences of the infringement shall be governed by the law expressly chosen by the parties; in absence of choice, it shall be governed by the law of common place of residence or main place of business; in absence of above common place, it shall be governed by the law of the country most closely connected.

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In the first section, the effect of sodium houttuyfonate on the level of lysozyme secreted by macrophages was first tested by bacteriolytic method; then the effect of sodium houttuyfonate on the level of tartaric acid resistant acid phosphatase produced in macrophages was investigated by analyzing the phenol generated in the reaction that was catalyzed by the enzyme; the third, the effect of sodium houttuyfonate on the phagocytic activity of macrophages was observed by microscopy; the forth, the effect of sodium houttuyfonate on the production of complement C4 from macrophages was tested by hemolytic method; the fifth, the effect of sodium houttuyfonate on the expression of C3b receptors on the surface of macrophages was analyzed by observing the formation percentage of zymosan wreath; the sixth, the effect of sodium houttuyfonate on the respiratory burst in macrophages was tested by flow cytometry; the seventh, the effect of sodium houttuyfonate on the production of IL-1α and IL-1β from macrophages was tested by ELISA method.

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Methods:Of 70 cases with traumatic pelvic fracture associated with injuries of other organ, 16 were treated by lienectomy, 7 were treated by liver repair, 6 were treated by colon operation, 6 were treated by intestine inosculation, 4 were treated by bladder fistulatome, 10 were treated by ligation of internal iliac arteries, 1 was treated by half-pelvic excision, 4 were treated by angioembolization, 5 were treated by conduction.

目的：探討外傷性骨盆骨折合并其他部位傷的診治方法及最佳適應(yīng)證方法：對71例外傷性骨盆骨折合并其他部位傷的病例進行不同方法的診治：脾切除16例国产一级黄色视频无码，肝修補7例，結(jié)腸手術(shù)6例亚洲第一中文字幕，小腸修補吻合6例，膀胱造瘺4例97无码免费人妻视频，髂內(nèi)動脈結(jié)扎10例国产操视频网，半骨盆離斷術(shù)1例，髂血管探查取栓4例一级国产精品，胸腔閉式引流5例四虎在线观看成人影院免费。

Methods:of 70 cases with traumatic pelvic fracture associated with injuries of other organ, 16 were treated by lienectomy, 7 were treated by liver repair, 6 were treated by colon operation, 6 were treated by intestine inosculation, 4 were treated by bladder fistulatome, 10 were treated by ligation of internal iliac arteries, 1 was treated by half-pelvic excision, 4 were treated by angioembolization, 5 were treated by conduction.results: totally 11 cases were dead, death rate was 15.5%.

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By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

為研制帶有O型口蹄疫病毒(Foot-and-Mouth Disease Virus亚洲玖玖在线第一页，F(xiàn)MDV)China99株結(jié)構(gòu)蛋白基因及多個非結(jié)構(gòu)蛋白基因的DNA疫苗亚洲小说图片网，本研究通過定點突變方法和PCR擴增方法，獲得包含有FMDV China99株結(jié)構(gòu)蛋白P1一级黄色变态在线视频、非結(jié)構(gòu)蛋白2A欧洲黄片免费看、3C以及部分2B、3B編碼基因的片段P12X3C和包含有FMDV China99株結(jié)構(gòu)蛋白P1丝袜脚交在线观看无码、非結(jié)構(gòu)蛋白2A亚洲色成a在线网、3C、3D以及部分2B台湾高清精品一二三四、3B編碼基因的片段P12X3C3D无国产A片马上打开，將獲得的基因片段直接/酶切后與同樣處理的真核表達質(zhì)粒連接，分別得到重組質(zhì)粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D中文字幕国产区、pTARGET/P12X3C3D人人操人人干人人玩；對重組質(zhì)粒進行序列測定、分析黄色.com，并將重組質(zhì)粒分別轉(zhuǎn)染BHK-21細胞欧美性爱网在线观看，通過雙抗體夾心ELISA方法和間接免疫熒光標(biāo)記方法檢測細胞中FMDV抗原的表達欧美乱图，用電子顯微鏡觀察病毒空衣殼的組裝；為評價重組質(zhì)粒作為DNA疫苗對實驗動物及本動物的免疫效果黄色一级成人网站免费，將重組質(zhì)粒經(jīng)肌肉注射方法接種豚鼠日韩美国一级欧美一级，并與酵母表達的純化FMDV China99株3D蛋白及帶有牛α干擾素的真核表達質(zhì)粒pcDNA3.1/IFN分別/同時免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株欧美色色.com；隨后將質(zhì)粒pcDNA3.1/P12X3C特殊黄色网、pcDNA3.1/P12X3C3D與帶有牛α干擾素的真核表達質(zhì)粒pcDNA3.1/IFN同時免疫牛，三周后經(jīng)牛舌皮攻以10〓ID〓的O型FMDV China99株欧美性交操B。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

為研制帶有O型口蹄疫病毒(Foot-and-Mouth Disease Virus免费在线观看三级毛片，F(xiàn)MDV)China99株結(jié)構(gòu)蛋白基因及多個非結(jié)構(gòu)蛋白基因的DNA疫曲，本研究通過定點突變方法和PCR擴增方法国产成人午夜福利影片在线播放网站，獲得包含有FMDV China99株結(jié)構(gòu)蛋白P1欧洲天堂综合网、非結(jié)構(gòu)蛋白2A、3C以及部分2B第一福利在线观看、3B編碼基因的片段P12X3C和包含有FMDV China99株結(jié)構(gòu)蛋白P1婷婷丁香五月天欧美中文、非結(jié)構(gòu)蛋白2A、3C美国操逼网站、3D以及部分2B男女猛烈无摭挡免费视频、3B編碼基因的片段P12X3C3D，將獲得的基因片段直接／酶切后與同樣處理的真核表達質(zhì)粒連接呦女网址，分別得到重組質(zhì)粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D亚洲色偷精品一区二区三区粗大猛、pTARGET／P12X3C3D；對重組質(zhì)粒進行序列測定国产精品va视频、分析不卡的av网站，并將重組質(zhì)粒分別轉(zhuǎn)染BHK-21細胞，通過雙抗體夾心ELISA方法和間接免疫熒光標(biāo)記方法檢測細胞中FMDV抗原的表達中文字幕永久永久在线，用電子顯微鏡觀察病毒空衣殼的組裝免费人成短视频观看网站最；為評價重組質(zhì)粒作為DNA疫苗對實驗動物及本動物的免疫效果操逼色图亚洲，將重組質(zhì)粒經(jīng)肌肉注射方法接種豚鼠春雨直播视频观看完整版高清，并與酵母表達的純化FMDV China99株3D蛋白及帶有牛α干擾素的真核表達質(zhì)粒pcDNA3.1／IFN分別／同時免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：隨后將質(zhì)粒pcDNA3.1／P12X3C亚洲欧美一区第一页、pcDNA3.1／P12X3C3D與帶有牛α干擾素的真核表達質(zhì)粒pcDNA3.1／IFN同時免疫牛免费高清性色毛片A片，三周后經(jīng)牛舌皮攻以10~4ID_(50)的O型FMDV China99株。