Methods Using the long-distance RT-PCR and a mammalian expression system, the full length cDNA of the α1D was cloned from cochleae and then expressed in HEK293 cell line.

以耳蝸基底膜中組織總RNA為模板黄色网站在线看免费版，利用長距離RT-PCR克隆耳蝸毛細胞表達的電壓門控鈣通道α1D剪切異構體黄片一级午夜福，并利用異源表達系統(tǒng)在哺乳動物細胞系進行表達特大巨黑吊XXXX高潮。

All these results suppose that COCH gene may play an architectural function in inner ear mesenchymic extracellular matrix, and this architectural stability is critical for the potassium recycling in cochleae and the normal level of endocochlear potential.

COCH基因功能的初步推論日韩欧美啪啪一中文字暮，COCH基因可能編碼內耳間質組織中一種分泌型糖蛋白，通過與間質組織中其它細胞外基質成分的結合嫖妓视频，共同形成穩(wěn)定的細胞外基質結構框架18岁免费黄。

The inner ear expressed a tissue-specific splicing isoform of α1D, and that the cell line constantly expressed this isoform can be used in physiologic and pharmacologic researches of L-type voltage-gated Ca2+ channel expressed in cochleae.

內耳毛細胞表達組織特異性的α1D剪切異構體，該異構體在外源表達系統(tǒng)中的表達為今后研究耳蝸α1D L-型鈣通道蛋白的電生理和藥理特性奠定了基礎www.一区二区三区.com。

Methods Using a combinatorial approach of enzymatic digestion and mechanical separation to allow isolation and culture of GER cells from P1 rat cochleae.

方法取生后1d大鼠耳蝸超碰人妻人人操人人干，利用機械分離和酶消化相結合的方法分離出純GER細胞，分別于無血清和含10％胎牛血清的DMEM培基中進行培養(yǎng)爆乳美女粉泬10p福利视频，觀察細胞生長特性视频二区亚洲，并對培養(yǎng)物進行溴脫氧尿苷、鬼筆環(huán)肽久久这里只精品免费、Z01亚洲444在线视频、鈣視網膜蛋白及肌球蛋白VIIa免疫組化鑒定及掃描電鏡觀察。

Then the animals were sacrificed and the surface preparation and the section of cochleae stained by MyosinⅥwere observed.Other cochlea were observed with SEM.

分別于術后第3.5個月分別行聽性腦干反應檢查后取雙側耳蝸標本做基底膜鋪片和耳蝸冰凍切片MyosinⅥ染色觀察有無新生毛細胞樣細胞出現羞羞免费网站，部分耳蝸作掃描電鏡觀察免费看黄页在线网站。

Kanic acid was injected into guinea pigs' cochleae and the excitotoxicity model was established. After a week the recombinant plasmid was transferred into SGCs of guinea pigs' cochlea treated with HAT. The following week the expression of NT-3 was examined by the immunohistochemical method, and the morphology of SGNs was observed under the electronic microscope after 4 weeks, in the mean time the changes of auditory brain-stein response were examined.

通過耳蝸灌注海人酸(kainic acid， KA)建立豚鼠耳蝸興奮性損傷模型最新国产成人野战视频在线观看免费，在給KA 1周后利用HAT攜帶重組質粒進行耳蝸灌注以轉染耳蝸螺旋神經節(jié)細胞好好日无码视频，免疫組化法觀察轉染后1周NT-3的表達及4周后電鏡下螺旋神經節(jié)細胞形態(tài)學變化，同時觀察對聽覺腦干誘發(fā)電位(auditory brain-stem response三级做a全过程在线观看视频， ABR)的影響男女www视频在线看网站。

Normal control group was also established. The effect of different medicines on the following parameters were detected by immunhistochemistry, biochemistrydetect method, histomorphometry, ect. Which were blood glucose ,TC, TG, HDL-C, blood and plasm viscosity , erythrocyte aggregation and rigidity index ,SOD ,MDA, ABR, changes in general condition and Cochleae as well.

通過生化檢測、免疫組化精选搞黄色视频的网站、形態(tài)學等方法中文精品日韩欧美在线播放，對照觀察各組藥物對SD大鼠血糖、TG98精品视频在线、HDL-C超碰人人99、全血粘度、血漿粘度中日韩午夜高青视频在线观看、紅細胞聚集指數美女自慰喷水的网站、紅細胞剛性指數、SOD麻豆av在线、MDA少妇熟妇亚洲综合网站、ABR等相關指標及耳蝸組織形態(tài)學的影響。

The infused and the contralateral, non-infused cochleae were harvested and stained with X-Gal, the Ad-LacZ infected cochlea were stained with blue color.

我們將Ad-lacZ病毒注入豚鼠內耳中日韩成人网址播放视频在线播放免费，分別在病毒注入二天5区av在线播放、一周、二周后取材固定進行X-Gal染色可觀察到免费在线观看国产黄片视频，整個耳蝸從蝸底到蝸尖均被染成蘭色久草视频99精品。

Mutations in COCH gene may impair the gap-junction between the spiral ligament fibrocytes, and obstruct the potassium recycling in cochleae, then, the endocochlear potential will be declined, hearing loss and vestibular dysfunction will be induced.

內耳尤其耳蝸間質組織中，這種穩(wěn)定的細胞外基質結構框架對維持鉀離子穩(wěn)態(tài)及蝸內電位水平具有重要意義欲色天天网综合久久88。

The cochleae of animals were dissected. propidium iodide, a dna intercalating fluorescent probe, was used to trace the morphological changes in ohc nuclei. f-actin staining was used to determine missing ohcs. the single strand dna in apoptotic ohcs of guinea pigs and mice, and apoptosis inducing factor and endonuclease g in ohcs of guinea pigs were examined by immunofluorescence method.

分離解剖耳蝸后视频亚洲日韩，用碘化丙啶染色細胞核、pholloidin染色f-actin婷婷激情五月综合，免疫熒光抗體分別染色單鏈dna亚洲天堂2020av、核酸內切酶g和凋亡誘導因子（apoptosis inducing factors， aif），制備耳蝸鋪片亚洲欧美中文字幕在线一区下载，激光共聚焦顯微鏡下觀察凋亡和壞死毛細胞內的熒光信號變化亚洲偷窥自拍。