Methods We isolated and cultured the MSCs, then induced MSCs into osteoblast and checked the osteoblast by RT-PCR; the conditioned medium in different concentration was prepared containing diphosphonate or assemble flavone of drynaria rhizome; with the conditioned medium the osteoblast was cultured; then the growth curve was analyzed and the cytogene (collagenase col Ⅰ) col Ⅰ and ALP expression was detected.

使用誘導(dǎo)方法作用于骨髓基質(zhì)干細(xì)胞毛片一级A片免费，使之轉(zhuǎn)化為成骨細(xì)胞av录像观看；用RT-PCR檢驗(yàn)成骨細(xì)胞欧美色污在线视频；不同濃度的二磷酸鹽或骨碎補(bǔ)總黃酮配制成條件培養(yǎng)基，作用于成骨細(xì)胞在线天堂官网首页网址，并分析生長(zhǎng)曲線一级片黄色网站，分析細(xì)胞基因堿性磷酸酶和膠原酶Ⅰ(collagenase col Ⅰ)表達(dá)情況。

Col.3:22 Servants, obey in all things your masters according to the flesh; not with eyeservice, as menpleasers; but in singleness of heart, fearing God: Col.3:23 And whatsoever ye do, do it heartily, as to the Lord, and not unto men; Col.3:24 Knowing that of the Lord ye shall receive the reward of the inheritance: for ye serve the Lord Christ.

col.3 ： 22公務(wù)員色拍拍555，服從在一切事上你的主人據(jù)肉中刺九九免费黄色视频，不與eyeservice ，不論男的女的，但在單一的心熟女熟妇伦aⅴ网站美利坚，不怕神： col.3 ： 23和葉什么做18岁以下禁止观看一级毛片，這樣做都由衷地，以耶和華jizzjizz小视频，不欲99久久久久久亚洲精品不卡，男； col.3 ： 24明知該葉公應(yīng)領(lǐng)取報(bào)酬的繼承：葉事奉耶和華基督女人高潮奶水喷出的视频。

The mRNA of CTGF, TGF-β1,ColⅠ and ColⅢ in neurofibroma tissue were over-expressed, whereas that in normal skin tissue was none or rare.

神經(jīng)纖維瘤組織及瘢痕疙瘩組織中CTGF欧美操屄视频网、TGF-β1、ColⅠ和ColⅢ的mRNA過表達(dá)亚洲欧美自拍日韩精品，而正常皮膚組織無或僅有極弱表達(dá)影音先锋性资源免费。

Antibodies against col Ⅲ reacted strongly in the entire pterygial stroma. Conclusions Components of ECM are changed in the pterygium: the sub-epithelial connective tissues and vessels are more prominent in the pterygia than in normal conjunctiva tissues, and abnormal fibers are visible only in the deeper subepithelial connective tissues. This study documents the overexpression of LN and col Ⅲ. They may play roles in cellular adherency, migration and angiogenesis. Also this study documents the overexpression of MMP3 which may degrade ECM in the pathogenesis of the pterygium.

結(jié)論翼狀胬肉組織中ECM的成分較正常球結(jié)膜發(fā)生改變，有大量的彈性纖維變性免费亚洲黄色、膠原纖維增生及新生血管形成日韩一区二区电影网站视频；col Ⅲ在翼狀胬肉組織中表達(dá)增加，LN及膠原纖維可能對(duì)翼狀胬肉中血管形成一级ar级99久久毛视频、細(xì)胞粘附和遷移及ECM重建起作用；MMP-3在翼狀胬肉組織中表達(dá)增加精品人妻综合文学，其對(duì)ECM成分的降解作用可能有助于翼狀胬肉的形成和向角膜中央侵襲成年人视频免费网站。

Results We can induce MSCs into osteoblasts which can express gene col Ⅰ and ALP. Through analysis growth curve, we demostrateed the diphosphonate or assemble flavone of drynaria rhizome with 10^(-4)~10^(-6)mmolL^(-1) had the inhibition on the osteomast; it with 10^(-8)mmolL^(-1) had a promotion on the osteoblast; it with 10^(-10)~10^(-12)mmolL^(-1) had a restraint on osteoblast. Conclusion Through gene analysis, we found the diphosphonate or assemble flavone of drynaria rhizome can have the promotion on the osteoblast.

結(jié)果 骨髓基質(zhì)細(xì)胞經(jīng)過4周誘導(dǎo)可轉(zhuǎn)化為表達(dá)colⅠ和ALP的成骨細(xì)胞，經(jīng)過生長(zhǎng)曲線分析99视频在线精品国自产拍免费观看，二磷酸鹽和骨碎補(bǔ)總黃酮10^(-4)~10^(-6)mmolL^(-1)時(shí)中文字幕无码一区在线一区，對(duì)誘導(dǎo)后的成骨細(xì)胞有抑制作用；10^(-8)molL^(-1)有促進(jìn)作用操人黄色性爱在线；10^(-10)~10^(-12)mmolL^(-1)不明顯亚洲乱伦熟妇，其中10^(-8)mmolL^(-1)作用最強(qiáng)；基因分析后亚洲影院在线观看播放，發(fā)現(xiàn)二磷酸鹽和骨碎補(bǔ)總黃酮促進(jìn)成骨細(xì)胞表達(dá)colⅠ和ALP国内久久久久久久久久精品影院，且兩者合用效果大于單獨(dú)用藥。

Cases healthy subjects served as control. In addition, density of glomerular matrix membrane and quantity of cell in glomeruli were measured with CMIAS image analysis system by computer. Global sclerosis, cellular crescents, fiber or fibrocellular crescents were measured with Memphis scope. Tubulointerstitial lesions were examined with semi-quantitative grades (including O-III grades).Results Compared to normal control, there was a significant increase of urinary TGF-betaK IL-6 and Col-lV levels in patients with IgAN (p. 01). There were also significantly positive correlation between the levels of urinary TGF-betaK Col-IV and density of glomerular matrix membrane ., interstitial fibrosisp.

采用酶聯(lián)免疫吸附法測(cè)定尿TGF-β1(Transforming growth factor-β1)自拍偷拍系列、尿IL-6(interleukin-6)和尿Col-Ⅳ(type Ⅳ Collagen)免费一级A毛片在线播放视频；采用免疫組織化學(xué)法檢測(cè)腎組織TGF-β1、Col-Ⅳ表達(dá)欧美孕妇三级片；應(yīng)用CMIAS多功能真彩色病理圖像分析系統(tǒng)對(duì)腎小球基質(zhì)基底膜面密度和細(xì)胞個(gè)數(shù)進(jìn)行半定量測(cè)定www、AⅤ毛片COm；對(duì)球性硬化、節(jié)段硬化黑人巨大精品欧美黑白配亚洲、細(xì)胞新月體及纖維或纖維細(xì)胞新月體所占腎小球百分?jǐn)?shù)和腎小球IgA沉積免疫熒光強(qiáng)弱的判斷采用Memphis法進(jìn)行評(píng)分国产原创资源网站；腎小管間質(zhì)病變程度采用光鏡下半定量分級(jí)法(包括0-Ⅲ級(jí))。

Theurinary IL-6 level positively correlated with density of glomerular matrix membrane, global sclerosis, fiber or fibrocellular crescents and interstitial fibrosis (p. 05). According to the degree of density of glomerular matrix membrane and interstitial fibrosis, urinary Col-IV level had better correlation than urinary TGF-betal and IL-6 levels.In IgAN, Col-IV showed increased expression in diseased renal tissue whereas the site of expression of TGF-betal was mainly localized within the cytoplasm of tubular epithelial cells. Interstial expressionwas also present but glomerular TGF-betal expression was found only in patients with heavy mesangial proliferation. There was a significant correlation between glomerular positivity for Col-IV and severity of histological damage. There was also a significant correlation between positivity for TGF-betal and Col-IV in the tubular epithelial and interstitial lesions. In contrast, there was no ralationship between glomerular positivity for TGF-betal and severity of histological damage.The urinary TGF-betal level paralleled tubular TGF-betal expression.

結(jié)果 ①IgAN患者尿TGF-β1、IL-691精品婷婷国产综合久久8、Col-Ⅳ水平較健康人明顯增高(P＜0.01)成人学生妹免费视频，該變化與血中的濃度無關(guān)(P＞0.05)；②尿TGF-β1水平與小管間質(zhì)TGF-β1陽性表達(dá)呈正相關(guān)(P=0.000)www.26uuu.com，而與小球TGF-β1陽性表達(dá)無關(guān)(P＞0.05)小蝌蚪国产，尿Col-Ⅳ水平與小球和小管間質(zhì)Col-Ⅳ陽性表達(dá)均呈良好的相關(guān)性(P＜0.01)，還與小管間質(zhì)TGF-β1陽性表達(dá)呈正相關(guān)(P＜0.05)：③小球Col-Ⅳ陽性表達(dá)與腎組織慢性病變密切相關(guān)(P＜0.05)欧美国产在线观看，小管間質(zhì)Col-Ⅳ和TGF-β1陽性表達(dá)均與腎小管間質(zhì)病變呈良好的相關(guān)性(P＜0.01)无遮挡又黄又爽又刺激的免费视频，而小球TGF-β1陽性表達(dá)與腎組織損傷無關(guān)(P＞0.05)；④尿TGF-β1最近免费中文字幕大全图片、Col-Ⅳ水平與腎小球基質(zhì)基底膜面密度污视频在线观看国产网站、小管間質(zhì)病變呈正相關(guān)(P＜0.01)，與小球內(nèi)細(xì)胞數(shù)呈負(fù)相關(guān)(P＜0.05)2021亚洲精品自拍视频，該結(jié)果與其在組織中的表達(dá)一致欧美在线特级黄片；尿IL-6水平浙江大學(xué)碩士學(xué)位論文尿TGF一B一、IL一6和Col一IV在IgA腎病中的應(yīng)用價(jià)值與基質(zhì)基底膜面密度人人舔人人干人人操、球性硬化午夜激情亚洲专区、纖維或細(xì)胞纖維新月體所占腎小球百分?jǐn)?shù)及小管間質(zhì)病變均有顯著的相關(guān)性(F.05)；在輕度腎病理損傷時(shí)玖玖爱在线视频精品日韩，尿'l'G卜pl肏逼免费视频、I卜6、Col一IV水平即升高理伦无码在线观看，而尿Col一W在反映細(xì)胞外基質(zhì)積聚和間質(zhì)纖維化程度上比尿TGF一pl和IL一6有更好的相關(guān)性黄色一及亚洲。

The main results were listed as follows:(1) Col-0 ecotype and the mutants were inoculated by Bipolaris oryzae, we discovered Col-0 ecotype was resistant to the isolation and vaccinal area emerged no spot; the mutants represented resistant.

將水稻胡麻斑病菌接種到擬南芥野生型和突變體植株上，發(fā)現(xiàn)野生型Col-0對(duì)病菌表現(xiàn)抗病欧美三级片免费久久，接種的葉片上沒有任何病斑日本三级性爱视频；而突變體植株則表現(xiàn)出不同程度的抗感差異。

MTT assay was used to evaluate the cytotoxicity of β-TCP/col on the proliferation of PDLCs. PDLCs were cultured on β-TCP/col and observed by scanning electron microscope.β-TCP/col was implated into muscles of nude mice to observe tissue reactions by histologic methods.Ⅱ° furcation involvements models in beagle dogs were established, scaffold and and scaffold with cells and cytokines were implanted into defects. Histologic observation were conducted.

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Sodium arsenite group and sodium arsenate group were made by 300 mg/L per day of sodium arsenite and sodium arsenate respectively. The mice were sacrificed after 10 months for liver function and pathologic examination. The expression of MMp-8, TIMP-2, Col-Ⅰh黄漫高潮喷水在线观看、Col-Ⅲ mRNA were detected with real time flourescence quantitative PCR. The result was controlled with 18s.

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